Search results for "Microscopy"

showing 10 items of 3390 documents

Binding mode analysis of ABCA7 for the prediction of novel Alzheimer's disease therapeutics

2021

Graphical abstract

ATP Adenosine-triphosphateNBD nucleotide binding domainGSH reduced glutathionePolypharmacologyAlzheimer’s disease (AD)ATP-binding cassette transporterHTS high-throughput screeningBiochemistryABCA7Structural BiologyPLIF protein ligand interactionMSD membrane spanning domainPDB protein data bankTM transmembrane helixABC ATP-binding cassetteMultitarget modulation (PANABC)RMSD root mean square distanceABC transporter (ABCA1 ABCA4 ABCA7)Computer Science ApplicationsMOE Molecular Operating EnvironmentPharmacophoreSNP single-nucleotide polymorphismBiotechnologyResearch ArticleBBB blood-brain barrierBiophysicsDrug designComputational biologyBiologyAD Alzheimer’s diseasePET positron emission tomographyIC intracellular helixAPP amyloid precursor proteincryo-EM cryogenic-electron microscopyGeneticsHomology modelingBinding siteRational drug design and developmentComputingMethodologies_COMPUTERGRAPHICSNBD-cholesterol 7-nitro-2-13-benzoxadiazol-4-yl-cholesterolTransporterPSO particle swarm optimizationPET tracer (PETABC)ECD extracellular domainR-domain/region regulatory domain/regionABCA1biology.proteinEH extracellular helixTP248.13-248.65BODIPY-cholesterol 44-difluoro-4-bora-3a4a-diaza-s-indacene-cholesterolComputational and Structural Biotechnology Journal
researchProduct

Study of the electronic and atomic structure of thermally treated SrTiO3(110) surfaces

2003

The electronic structure of heated SrTiO3(110) surfaces was investigated with metastable impact electron spectroscopy and ultraviolet photoelectron spectroscopy (He(I). Scanning tunnelling microscopy and atomic force microscopy (AFM) were used to study the topology of the surface. The crystals were heated up to 1000 °C under reducing conditions in ultrahigh vacuum or under oxidizing conditions in synthetic air for 1 h, respectively. Under both conditions microfacetting of the surface is observed. The experimental results are compared with ab initio Hartree-Fock calculations, also presented here, carried out for both ideal and reconstructed SrTiO 3(110) surfaces. The results give direct evid…

Ab initioAnalytical chemistrySurfaces and InterfacesGeneral ChemistryElectronic structureCondensed Matter PhysicsElectron spectroscopySurfaces Coatings and Filmslaw.inventionCondensed Matter::Materials Sciencechemistry.chemical_compoundchemistryAb initio quantum chemistry methodslawMicroscopyMaterials ChemistryStrontium titanateAtomic physicsScanning tunneling microscopeUltraviolet photoelectron spectroscopySurface and Interface Analysis
researchProduct

Intra-abdominal

2016

Aims To evaluate the contribution of light microscopy to detecting Candida spp infection in patients with complicated intra-abdominal infections (IAIs) admitted for acute abdomen to a quality assurance (QA)-certified surgical emergency ward. Methods We conducted a retrospective study (2008–2012) of 809 abdominal intraoperative or biopsy tissue specimens obtained from patients admitted with acute abdomen and microbiological samples positive for Candida spp. Demographic data, mortality, comorbidities, specimen type, microscopy results, special histological staining performed, antimicrobial therapy were collected and analysed. Any comments at the multidisciplinary team meeting was recorded in …

Abdomen AcuteAdultAged 80 and overMaleMicroscopyAntifungal AgentsQuality Assurance Health CareCandidiasisFUNGIMiddle AgedGASTROENTEROLOGYEchinocandinsYoung AdultINFECTIONSHumansINFECTIOUS INTESTINAL DISEASEFemaleOriginal Article1506FluconazoleAgedCandidaRetrospective StudiesJournal of clinical pathology
researchProduct

Hybrid layers of etch-and-rinse versus self-etching adhesive systems.

2010

Objective: To evaluate the effectiveness in the formation of resin tags, adhesive lateral branches and hybrid layers of five adhesive systems, when bonding to dentine. Material and Methods: Flat dentin surfaces from 25 molars were bonded with several adhesive systems according to the manufacturers? instructions. Composite build-ups were constructed incrementally with Tetric Ceram. The Specimens were sectioned parallel to the long axis. One section was treated with phosphoric acid and sodium hypochlorite in order to reveal the hybrid layer formation. The other section was stored in 30% hydrochloric acid to detect resin tags and adhesive lateral branch formation. Results: The two etch-and-rin…

Absorption of waterMaterials scienceScanning electron microscopeComposite numberDental Cementschemistry.chemical_compoundstomatognathic systemDentinmedicineHumansComposite materialGeneral DentistryBondingtechnology industry and agricultureDental Bonding:CIENCIAS MÉDICAS [UNESCO]medicine.anatomical_structureOtorhinolaryngologyPolymerizationchemistrySodium hypochloriteUNESCO::CIENCIAS MÉDICASDentinDental EtchingMicroscopy Electron ScanningSurgeryAdhesiveScanning electron microscopyLayer (electronics)Bonding Dentin Scanning electron microscopy
researchProduct

Pd–M/C (M = Pd, Cu, Pt) Electrocatalysts for Oxygen Reduction Reaction in Alkaline Medium: Correlating the Electronic Structure with Activity

2017

The increasing global needs for clean and renewable energy have fostered the design of new and highly efficient materials for fuel cells applications. In this work, Pd-M (M = Pd, Cu, Pt) and Pt nanoparticles were prepared by a green synthesis method. The carbon-supported nanoparticles were evaluated as electrocatalysts for the oxygen reduction reaction (ORR) in alkaline medium. A comprehensive electronic and structural characterization of these materials was achieved using X-ray diffraction, high-resolution transmission electron microscopy, X-ray photoelectron spectroscopy, and X-ray absorption spectroscopy. Their electrochemical properties were investigated by cyclic voltammetry, while the…

Absorption spectroscopyChemistryInorganic chemistryNanoparticle02 engineering and technologySurfaces and Interfaces010402 general chemistry021001 nanoscience & nanotechnologyCondensed Matter PhysicsElectrochemistry01 natural sciences0104 chemical sciencesX-ray photoelectron spectroscopyTransmission electron microscopyElectrochemistryGeneral Materials ScienceCyclic voltammetry0210 nano-technologyPolarization (electrochemistry)Bimetallic stripSpectroscopyLangmuir
researchProduct

Metabolic shift of polyphosphate-accumulating organisms with different levels of polyphosphate storage

2012

Previous studies have shown that polyphosphate-accumulating organisms (PAOs) are able to behave as glycogen-accumulating organisms (GAOs) under different conditions. In this study we investigated the behavior of a culture enriched with Accumulibacter at different levels of polyphosphate (poly-P) storage. The results of stoichiometric ratios Gly degraded/HAc uptake, PHB synthesized/HAc uptake, PHV synthesized/HAc uptake and P release/HAc uptake confirmed a metabolic shift from PAO metabolism to GAO metabolism: PAOs with high poly-P content used the poly-P to obtain adenosine tri-phosphate (ATP), and glycogen (Gly) to obtain nicotinamide adenine dinucleotide (NADH) and some ATP. In a test whe…

Accumulibacter Type IIWaste component removalUnclassified drugPhysiologyChemical compositionMicrobial metabolismStorageWastewaterNicotinamide adenine dinucleotidePolyhydroxyalkanoic acidchemistry.chemical_compoundBacteriumBioreactorsPolyphosphatesGlycolysisAnaerobiosisBiomassPolyphosphate-accumulating organismsWaste Management and DisposalAccumulibacter Type IGlycogen accumulating organismPriority journalWater Science and TechnologyFluorescence microscopyPolyhydroxyvalerateSewageGlycogenHydrolysisFluorescence in situ hybridizationEcological ModelingPhosphorusHydrogen-Ion ConcentrationBioaccumulationPollutionStoichiometryWaste treatmentPolyphosphate-accumulating organismsBiodegradation EnvironmentalEnhanced biological phosphorus removalBiochemistryGlycogen-accumulating metabolism (GAM)Nicotinamide adenine dinucleotideAccumulibacter type 1Accumulibacter type 2GlycolysisGlycogenMetabolic Networks and PathwaysAccumulibacterAdenosine triphosphateEnvironmental EngineeringBiologyAcetic acidArticleAssociative storagePolyphosphate-accumulating metabolism (PAM)PolyphosphateGlycogen-accumulating organismsGlycogen-accumulating metabolismsTECNOLOGIA DEL MEDIO AMBIENTEPolyphosphate accumulating organismCivil and Structural EngineeringPolyphosphate-accumulating organisms (PAO)BacteriaPolyphosphateMetabolismIn situ measurementGlycogen-accumulating organisms (GAO)Polyphosphate-accumulating metabolismsNonhumanAmidesCarbonMetabolismchemistryPolyphosphate (poly-P)Bacterial metabolismCell cultureVolatilizationWater Research
researchProduct

Characterization of particle morphology of biochanin A molecularly imprinted polymers and their properties as a potential sorbent for solid-phase ext…

2014

Abstract Molecularly imprinted polymers (MIPs) with biochanin A as a template were obtained using a bulk polymerization with non-covalent imprinting approach. The polymers were prepared in acetonitrile as porogen, using ethylene glycol dimethacrylate (EDMA) as cross-linking agent. The synthesis, with an application of 1′,1′-azobis(cyclohexanecarbonitrile) (ACHN) as an initiator, has been performed thermally. During the synthesis process the effect of different functional monomers such as methacrylic acid (MAA), acrylamide (AA) and 4-vinylpyridine (4-VP) was investigated. The application of nitrogen sorption porosimetry, scanning electron microscopy (SEM), and Fourier transform infrared spec…

AcetonitrilesMaterials scienceNitrogenPolymersPyridinesEthylene glycol dimethacrylateBioengineeringPolymerizationBiochanin ABiomaterialschemistry.chemical_compoundSpectroscopy Fourier Transform Infraredsolid-phase extractionSolid phase extractionFourier transform infrared spectroscopyChromatography High Pressure Liquidchemistry.chemical_classificationphytoestrogensAcrylamideChromatographySolid Phase ExtractionMolecularly imprinted polymerPolymerGenisteinIsoflavonesCross-Linking ReagentschemistryMethacrylic acidMechanics of MaterialsadsorptionMicroscopy Electron ScanningMethacrylatesmolecular imprintingMolecular imprintingNuclear chemistryMaterials Science & Engineering C-Materials for Biological Applications
researchProduct

Effects of electrical stimulation of the superior cervical ganglia on the number of ?synaptic? ribbons and the activity of melatonin-forming enzymes …

1989

Melatonin metabolism in the mammalian pineal gland is under the clear influence of sympathetic fibers originating in the superior cervical ganglia (SCG). Previous studies suggested that pineal "synaptic" ribbons (SR) as well are regulated by the gland's sympathetic innervation. To gain more insight into the mechanisms involved, we examined the effects of sympathetic stimulation on SR number and on the activity of melatonin forming enzymes, serotonin N-acetyltransferase (NAT) and hydroxyindole-O-methyltransferase (HIOMT). The SCG in adult male rats were stimulated electrically during daytime for either 15 or 120 min. Immediately following stimulation, the glands were removed and processed fo…

Acetylserotonin O-MethyltransferaseMaleNervous systemendocrine systemEmbryologySuperior cervical ganglionmedicine.medical_specialtyArylamine N-AcetyltransferaseStimulationBiologyPineal GlandMelatoninPineal glandInternal medicinemedicineAnimalsMelatoninGanglia SympatheticRats Inbred StrainsCell BiologyMetabolismElectric StimulationRatsMicroscopy Electronmedicine.anatomical_structureEndocrinologySynapsesCervical gangliaSerotoninAnatomyDevelopmental Biologymedicine.drugAnatomy and Embryology
researchProduct

The pineal complex in Roman high avoidance and Roman low avoidance rats.

1990

Previous studies have shown that the pineal gland of Roman high avoidance (RHA/Verh) rats is larger than that of Roman low avoidance rats (RLA/Verh). In the present study measurement of enzyme activities (serotonin-N-acetyl-transferase, hydroxyindole-O-methyltransferase) revealed that pineals of RHA/Verh rats are twice as active in melatonin production than pineals of RLA/Verh rats. Indoleamine content was also higher in RHA/Verh rats, whereas noradrenaline content was the same in both lines. When values were expressed per mg protein, these differences disappeared except for N-acetyl-serotonin and noradrenaline which were higher or lower in RHA/Verh rats, respectively. Both lines had higher…

Acetylserotonin O-MethyltransferaseMaleendocrine systemmedicine.medical_specialtyArylamine N-AcetyltransferaseBiologyPineal GlandPinealocyteMelatoninPineal glandAcetyltransferasesInternal medicinemedicineAvoidance LearningAnimalsCircadian rhythmElectron microscopicBiological PsychiatryMelatoninRadioimmunoassayRats Inbred StrainsMethyltransferasesRatsPsychiatry and Mental healthMicroscopy ElectronEndocrinologymedicine.anatomical_structurenervous systemNeurologyAcetylserotonin O-methyltransferaseSynapsesNeurology (clinical)Dark phasehormones hormone substitutes and hormone antagonistsmedicine.drugJournal of neural transmission. General section
researchProduct

Achlya spiralis, a new aquatic oomycete with bent oogonial stalks, isolated from the Burgundian region of France

2008

Achlya spiralis sp. nov. was isolated from water samples collected in the river Tille in the Burgundian region of France. The new oomycete is described, illustrated and compared with related species of the genus Achlya. It is characterized by the presence of smooth-walled oogonia that are usually borne on bent or twisted oogonial stalks; mainly monoclinous, androgynous and diclinous antheridial branches and eccentric oospores which generally do not mature or mature after a long period of time. The internal transcribed spacer (ITS) region of its rRNA is comprised of 671 bases. The taxonomic description of this new species, its comparison with related oomycetes and the sequence of the ITS reg…

Achlya spiralisAntheridiaMolecular Sequence DataFresh WaterMicrobiologyDNA AlgalOogoniaRiversGenusDNA Ribosomal SpacerBotanyGeneticsAchlya spiralisCiencias NaturalesInternal transcribed spacerrRNAMolecular BiologyOomyceteMicroscopybiologyITS regionSequence Analysis DNAAchlyaOosporesRibosomal RNAbiology.organism_classificationAchlyaAntheridiumOosporeFrance
researchProduct