Search results for "Microscopy"
showing 10 items of 3390 documents
Endoscope‐microscope combination for endonasal sinus surgery
1998
Advances in endoscopic imaging of colorectal neoplasia.
2010
Colon cancer screening is arguably the most important activity performed by gastroenterologists. Recent decreases in rates of death from colorectal cancer indicate that screening methods such as colonoscopy have a positive impact. There is still room for improvement, however, particularly in prevention of right-sided colon cancer. Practice issues, such as making colonoscopy more comfortable, safer, and less costly, are keys to continued success in cancer prevention. Colonoscopy techniques, technologies, and quality control measures have advanced to improve detection, classification, and removal of early neoplasias. In particular, slow, careful inspection of the colon by gastroenterologists …
Improvement in Nuclear Entry and Transgene Expression of Baculoviruses by Disintegration of Microtubules in Human Hepatocytes
2005
ABSTRACT Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV), a potent virus for mammalian cell gene delivery, possesses an ability to transduce mammalian cells without viral replication. We examined the role of the cellular cytoskeleton in the cytoplasmic trafficking of viral particles toward the nucleus in human hepatic cells. Microscopic studies showed that capsids were found in the nucleus after either viral inoculation or cytoplasmic microinjection of nucleocapsids. The presence of microtubule (MT) depolymerizing agents caused the amount of nuclear capsids to increase. Overexpression of p50/dynamitin, an inhibitor of dynein-dependent endocytic trafficking from peripheral e…
γ2-Adaptin is functioning in the late endosomal sorting pathway and interacts with ESCRT-I and -III subunits.
2010
Abstractγ2-Adaptin is a clathrin adaptor-related protein with unclear physiological function. Previous studies indicated that γ2-adaptin might act within the multivesicular body (MVB) protein-sorting pathway that is central to receptor down-regulation, lysosome biogenesis, and budding of enveloped viruses. Here, we have analyzed the effects of excess and deficit γ2-adaptin on exogenous and endogenous MVB cargoes and on the MVB machinery itself. Foreign cargoes, like retroviral Gags, are entrapped by overexpressed γ2-adaptin in detergent-insoluble polymers and blocked in budding. When viral budding involves MVB/endosomal structures, excess γ2-adaptin acts by accelerating lysosomal Gag destru…
Impact of Ceramic and Metallic Nano-scaled Particles on Endothelial Cell Functionsin vitro
2007
The sections in this article are Introduction Origin of Particles in the Human Environment Evidence for Size-dependent Toxicity of Particles Dissemination and Interferences of Nanoparticles within the Body Endothelial Cells and Nanoparticle Exposure Testing of Nanoparticle-induced Effects on Human Endothelial Cells In Vitro Materials and Methods Cell Culture Particles Transmission Electron Microscopy (TEM) Cytotoxicity Assay Detection of Ki67 Expression Quantification of IL-8 Release in Cell Culture Supernatant Quantification of E-selectin Cell Surface Protein Expression Fluorescence Staining Statistical Analysis Results Discussion Particle Internalization Particle Cytotoxicity Pro-inflamma…
Lymphatic endothelial progenitors bud from the cardinal vein and intersomitic vessels in mammalian embryos.
2012
Abstract The lymphatic vasculature preserves tissue fluid balance by absorbing fluid and macromolecules and transporting them to the blood vessels for circulation. The stepwise process leading to the formation of the mammalian lymphatic vasculature starts by the expression of the gene Prox1 in a subpopulation of blood endothelial cells (BECs) on the cardinal vein (CV) at approximately E9.5. These Prox1-expressing lymphatic endothelial cells (LECs) will exit the CV to form lymph sacs, primitive structures from which the entire lymphatic network is derived. Until now, no conclusive information was available regarding the cellular processes by which these LEC progenitors exit the CV without co…
Anti-inflammatory properties of simvastatin on leukocyte-endothelial cell interactions following hemorrhagic shock
2002
Background Hydroxymethylglutaryl coenzyme A (HMG-CoA) reductase inhibitors (statins) have been shown to lower serum cholesterol levels. Recent studies reported that statins have vasculoprotective effects independent of their cholesterol-lowering properties. We studied simvastatin for its ability to modulate leukocyte-endothelial cell interactions under acute inflammation following hemorrhage and reperfusion. Methods The effects of simvastatin on leukocyte-endothelial cell interactions were observed by intravital microscopy in the rat mesenteric microcirculation and by immunohistochemical analyzes. Simvastatin (50 μg/kg or 100 μg/kg) was administrated intraperitoneally 18 h before study. Inf…
Geomaterials in green building practices: comparative characterization of commercially available clay-based plasters
2013
Three pre-mixed powdered clay-based (earthen) plasters produced in Europe and specifically designed for wall undercoating were analyzed in this paper. These materials are commercially available and successfully employed in green building practices all over the world. Their compositional and textural characteristics, as well as plastic behaviour were investigated through a multi-analytical approach: X-ray powder diffraction (XRPD), polarized light microscopy (PLM), X-ray fluorescence spectrometry (XRFS), scanning electron microscopy equipped with an energy dispersive spectrometer (SEM-EDS), mercury intrusion porosimetry (MIP), grain-size distribution (GSD) and semi-empirical tests (Atterberg…
Characterization of the Etna volcanic emissions through an active biomonitoring technique (moss-bags): Part 2 – Morphological and mineralogical featu…
2013
Volcanic emissions were studied at Mount Etna (Italy) by using moss-bags technique. Mosses were exposed around the volcano at different distances from the active vents to evaluate the impact of volcanic emissions in the atmosphere. Morphology and mineralogy of volcanic particulate intercepted by mosses were investigated using scanning electron microscopy (SEM) equipped with energy dispersive spectrometer (EDS). Particles emitted during passive degassing activity from the two active vents, Bocca Nuova and North East Crater (BNC and NEC), were identified as silicates, sulfates and halide compounds. In addition to volcanic particles, we found evidences also of geogenic, anthropogenic and marin…