Search results for "Microspore"
showing 5 items of 35 documents
Influence of Microspore Development on Pollen Protoplast Isolation in Digitalis obscura
1991
Summary Protocols for the enzymatic isolation of Digitalis obscura pollen protoplasts are described. The developmental stage of the microspores was a critical factor for successful isolation since protoplasts were obtained only from tetrads. The highest yield of viable protoplasts was achieved with an enzymatic mixture containing helicase, cellulase and pectolyase in 0.5 M sucrose. In different culture techniques employed, cell wall regeneration took place only when protoplasts were plated in agarose drops.
Ploidy manipulation for genetic improvement in some Mediterranean fruit crops
2017
Plant breeding is focused on selection of new genotypes with improved traits. Conventional methods based on hybridization and those based on biotechnology (somatic hybridization, genetic transformation, ploidy manipulation, etc.) are used to create novel genetic variations. Biotechnology provides powerful tools for plant breeding, for instance, haploid technology allows achievement of homozygous lines from heterozygous parents in one step, which reduces significantly the time required by conventional methods. Concerning woody species, characterized by self-incompatibility, long juvenile period and high degree of heterozygosity, this technique is the only way to get homozygous lines. Haploid…
Generation of homozygosity and genome fixation in pea (Pisum sativum L.)
2013
Pea cultivars are nearly homozygous and thus homogeneous when they are released. The traditional method of selfing is slow and inefficient, taking up to ten generations of inbreeding following a cross to achieve a high level of homozygosity. Current single-seed-descent (SSD) methodologies enable a maximum of three generations per year to be developed in pea. Doubled haploidy and an in vitro based modified SSD technology have been utilised in many important crops for the rapid achievement of homozygosity, and thus acceleration of the breeding process. In pea, due to the lack of robust protocols, none of these technologies is routinely used in a breeding program. The aim of this study was to …