Search results for "Moduli"
showing 10 items of 206 documents
The structural plasticity of the C terminus of p21Cip1 is a determinant for target protein recognition.
2003
The cyclin-dependent kinase inhibitory protein p21(Cip1) might play multiple roles in cell-cycle regulation through interaction of its C-terminal domain with a defined set of cellular proteins such as proliferating cell nuclear antigen (PCNA), calmodulin (CaM), and the oncoprotein SET. p21(Cip1) could be described as an intrinsically unstructured protein in solution although the C-terminal domain adopts a well-defined extended conformation when bound to PCNA. However, the molecular mechanism of the interaction with CaM and the oncoprotein SET is not well understood, partly because of the lack of structural information. In this work, a peptide derived from the C-terminal domain of p21(Cip1) …
Interaction of Mitogen-activated Protein Kinases with the Kinase Interaction Motif of the Tyrosine Phosphatase PTP-SL Provides Substrate Specificity …
1999
ERK1 and ERK2 associate with the tyrosine phosphatase PTP-SL through a kinase interaction motif (KIM) located in the juxtamembrane region of PTP-SL. A glutathione S-transferase (GST)-PTP-SL fusion protein containing the KIM associated with ERK1 and ERK2 as well as with p38/HOG, but not with the related JNK1 kinase or with protein kinase A or C. Accordingly, ERK2 showed in vitro substrate specificity to phosphorylate GST-PTP-SL in comparison with GST-c-Jun. Furthermore, tyrosine dephosphorylation of ERK2 by the PTP-SLDeltaKIM mutant was impaired. The in vitro association of ERK1/2 with GST-PTP-SL was highly stable; however, low concentrations of nucleotides partially dissociated the ERK1/2.P…
Origin of the integrin-mediated signal transduction. Functional studies with cell cultures from the sponge Suberites domuncula
1999
Sponges (phylum Porifera) represent the phylogenetically oldest metazoan animals. Recently, from the marine sponge Geodia cydonium a first cDNA encoding a putative integrin receptor molecule was isolated. In the present study basic functional experiments have been conducted to test the hypothesis that in sponges integrin polypeptides also function as adhesion molecules and as outside-in signaling molecules. The sponge Suberites domuncula has been used for the experiments because from this sponge only has a cell culture been established. Here we report that aggregation factor (AF)-mediated cell-cell adhesion is blocked by the RGDS peptide which is known to interact with beta integrin. Both R…
Evolutionary relationships of Metazoa within the eukaryotes based on molecular data from Porifera
1999
Recent molecular data provide strong support for the view that all metazoan phyla, including Porifera, are of monophyletic origin. The relationship of Metazoa, including the Porifera, to Plantae, Fungi and unicellular eukaryotes has only rarely been studied by using cDNAs coding for proteins. Sequence data from rDNA suggested a relationship of Porifera to unicellular eukaryotes (choanoflagellates). However, ultrastructural studies of choanocytes did not support these findings. In the present study, we compared amino acid sequences that are found in a variety of metazoans (including sponges) with those of Plantae, Fungi and unicellular eukaryotes, to obtain an answer to this question. We use…
Akustiskās vadības modulis
2022
Kvalifikācijas darba mērķis ir izpētīt un izstrādāt moduli, kurš pārveidotu balss komandu programmatūrai saprotamā tekstuālā formātā. Šāda moduļa izstrāde būtu izmantojama citos projektos, kuros būtu nepieciešama šāda funkcionalitāte, kā arī moduli un tā izveidē iegūtās zināšanas var izmantot tā tālākā attīstībā un pilnvērtīgā balss vadītu risinājumu izstrādē. Modulis strādā ar bez relāciju datubāzi. Sistēma ir izstrādāta Python un C++ programmēšanas valodās. Atslēgvārdi: kvalifikācijas darbs, no runas par tekstu, implementējams modulis, mašīnmācība.
Lietvedības sistēmas papildināšana ar klienta pārvaldīšanas moduli
2019
Kvalifikācijas darba mērķis ir izstrādāt klienta pārvaldīšanas moduli lietvedības sistēmai un integrēt to pašā sistēmā. Moduļi ir lietvedības SharePoint serverī izsaucama aplikācija, kas spēj pārvaldīt lietvedības sistēmas konfigurācijas dotām klientam un pielietot tos sistēmai. Modulī tiek integrēts žurnāls, kas ļauj apskatīt klienta darbību vēsturi gan lietvedības sistēmā, gan pašā modulī. Modulim pastāv administratora lietotājs, kuram ir papildināta funkcionalitāte, salīdzinot ar parasto klientu. Administratora nolūks ir tehniska atbalsta sniegšana klientam. Viņam ir iespējas pārvaldīt citu lietotāju konfigurācijas, pielietot tos, pārskatīt viņu žurnālus, veidot un dzēst jaunus lietotāju…
Publisko datu sistēmas administrēšanas moduļa uzlabošana
2016
Darbā ir aprakstīts Valsts ieņēmumu dienesta publiskās datu bāzes administrēšanas moduļa uzlabošana, izmantojot Modelis-Skats-Kontrolleris (MVC) šablonu. Programmējot izmantota objektorientētā pieeja. Sistēmas pamatbūtība ir ļaut administratoram veikt pārskatus par ierakstu skaitiem gan oriģinālajā datu bāzē, gan publiski pieejamajā datu bāzē.
Comment on “High-pressure x-ray diffraction study of YBO3/Eu3+, GdBO3, and EuBO3: Pressure-induced amorphization in GdBO3” [J. Appl. Phys. 115, 04350…
2014
High-pressure x-ray diffraction studies on vaterite-type borates were reported on the above paper and their room-temperature P-V equation of state (EOS) determined. YBO3/Eu3+ and GdBO3 were found to have bulk moduli around 320 GPa, 90% larger than the bulk modulus obtained for EuBO3. Consequently, it was stated that vaterite-type borates are as incompressible as cubic BN. Such a different compressional behavior of isomorphic borates contradicts the known systematic of related borates. Here, we show that the conclusions reported on the above article could be hindered by experimental errors and artifacts. Ab initio calculations support our criticism giving similar bulk moduli (130–141 GPa) in…
GdBO3 and YBO3 crystals under compression
2021
High-pressure X-ray diffraction studies on nanocrystals of the GdBO3 and YBO3 rare-earth orthoborates are herein reported up to 17.4(2) and 13.4(2) GPa respectively. The subsequent determination of the room-temperature pressure-volume equations of state is presented and discussed in the context of contemporary publications which contradict the findings of this work. In particular, the isothermal bulk moduli of GdBO3 and YBO3 are found to be 170(13) and 163(13) GPa respectively, almost 50% smaller than recent findings. Our experimental results provide an accurate revision of the high-pressure compressibility behaviour of GdBO3 and YBO3 which is consistent with the known systematics in isomor…
Shear moduli of two dimensional binary glasses
2012
The shear moduli of two-component glasses in two dimensions are studied within mode coupling theory. Varying the concentration, strong mixing effects are observed along the glass transition lines for two interaction potentials. Nonoverlapping disks with size ratios between 0.3 and 0.9, and point particles interacting with (magnetic) dipoles of strength ratio between 0.1 and 0.6 are considered. Equilibrium structure factors (partially obtained from Monte Carlo simulations) and glass form factors, and perturbative calculations show that a softening of the elastic shear constant of glass upon adding another component arises from a dilution effect of the majority component. For very disparate m…