Search results for "Molecular analysis"

Molecular analysis of TP53, Ki-Ras and P16 methylation status in tissue and plasma of subjects affected by gastrointestinal cancer (GIC)

2007

BACKGROUND: Despite the improvement in detection and surgical therapy in the last years, the outcome of patients affected by colorectal carcinoma (CRC) remains limited by metastatic relapse. The aim of this study was to investigate the presence of free tumor DNA in the plasma of CRC patients in order to understand its possible prognostic role. PATIENTS AND METHODS: Ki-Ras, TP53 mutations and p16(INK4A) methylation status were prospectively evaluated in tumor tissues and plasma of 66 CRC patients. RESULTS: In 50 of the 66 primitive tumor cases (76%) at least one significant alteration was identified in Ki-Ras and/or TP53 and/or p16(INK4A) genes. Eighteen of the 50 patients presented the same…

Chaperons moleculae in brain tumors-CHAMOBRAT TRIAL: HSP60 and microRNAs related levels in tissue and circulating exosomes in human brain tumors befo…

Current regimen for high-grade gliomas is maximal safe surgical resection followed by external beam radiotherapy with concurrent temozolamide. Maximal tumor resection, however, must be balanced with preservation of the patient’s neurological function. A crucial prognostic factor in oncological neurosurgery is the extent of resection. Several studies have addressed the importance of extent of resection in gliomas surgery. Despite development in the fields of pre operative and intraoperative neuroimaging and neuromonitoring have ameliorated the survival rate and the quality of life for patients affected by high grade gliomas, the clinical outcome of patients with such gliomas remains extremel…

Molecular and taxonomic characterization of a endophytic fungus isolated from Helleborus bocconei subsp. intermedius (Ranunculaceae)

2014

A non-sporulating fungus was isolated from different organs of Helleborus bocconei subsp. intermedius (Ranunculaceae) endemic to southern Italy and Sicily, known for the traditional use of dried roots in the treatment of lung diseases of cattle and horses. Molecular characterization of endophytic fungus based on the internal transcribed spacer (ITS) region of the rRNA gene sequences was done. The DNA sequence of full length ITS region of the studied fungus was a 100% match to that of Chaetomium strumarium strain dH 21642 (GenBank accession number JX280851.1). The morphological characters of colony and mycelium of this microfungus are reported here.

CoproID predicts the source of coprolites and paleofeces using microbiome composition and host DNA content

2020

Shotgun metagenomics applied to archaeological feces (paleofeces) can bring new insights into the composition and functions of human and animal gut microbiota from the past. However, paleofeces often undergo physical distortions in archaeological sediments, making their source species difficult to identify on the basis of fecal morphology or microscopic features alone. Here we present a reproducible and scalable pipeline using both host and microbial DNA to infer the host source of fecal material. We apply this pipeline to newly sequenced archaeological specimens and show that we are able to distinguish morphologically similar human and canine paleofeces, as well as non-fecal sediments, fro…

Integrative Taxonomy of Armeria arenaria (Plumbaginaceae), with a Special Focus on the Putative Subspecies Endemic to the Apennines

2022

Three subspecies of Armeria arenaria are reported from Italy, two of which are considered endemic to the Apennines. The taxonomic value of these two taxa (A. arenaria subsp. marginata and A. arenaria subsp. apennina) is unclear. Moreover, the relationships between A. arenaria subsp. praecox and Northern Italian populations—currently ascribed to A. arenaria subsp. arenaria—have never been addressed. Accordingly, we used an integrated taxonomic approach, including morphometry, seed morpho–colorimetry, karyology, molecular systematics (psbA–trnH, trnQ–rps16, trnF–trnL, trnL–rpl32, and ITS region), and comparative niche analysis. According to our result…

Induction of Apoptosis and Chemosensitization by the Histone Deacetylase Inhibitor Trichostatin in Hepatocellular Carcinoma Cells: Molecular Analysis…

2011

The mRNA and protein levels of RKIP are reduced and those of YY1 increased in clinical HCC. Loss, mutation, or promoter hypermethylation of the RKIP gene may not account for the downregulation of RKIP in HCC. Histone deacetylation can silence gene expression and play a significant role in hepatocarcinogenesis. The histone deacetylase inhibitor (HDACI) trichostatin induced cell growth inhibition and proapoptotic effects in HA22T/VGH and HepG2 HCC cells; it also exhibited synergy with doxorubicin. Treatment with trichostatin caused histone hyperacetylation and down- or upregulated expression of different genes (such as β-catenin, cyclin D1, hTERT, XIAP, and IL-6). These changes might, at leas…

Isolation of Central Nervous System (CNS) Infiltrating Cells

2014

Leukocyte infiltration of the central nervous system (CNS) occurs under certain pathogenic conditions and most often results in severe disorders. Therefore, the isolation and analysis of such infiltrating cell populations is necessary for elucidating the underlying pathogenic mechanisms. Here we describe a simple and straightforward protocol for cell isolation from the inflamed CNS, which combines mechanical dissociation and enzymatic degradation of the tissue. Additionally, purification by Percoll gradient centrifugation provides a great yield of the infiltrating material. The isolated cells can be further used for downstream applications such as cell sorting, cellular or molecular analysi…

16P Molecular analysis for precision oncology of children: Beyond genomics

2020

Molecular Analysis of the Androgen Receptor Gene in 52 Patients with Complete or Partial Androgen Insensitivity Syndrome: A Collaborative Study

1992

In patients with androgen insensitivity syndrome (AIS), RFLP study of the androgen receptor gene made it possible to analyze whether deletions or mutations could be responsible for abnormalities in androgen responsiveness. We studied RFLPs of DNA from 25 46,XY patients with partial AIS (PAIS), defined as a concentration of androgen receptor in genital-skin fibroblasts less than 340 fmol/mg DNA, and DNA from 27 46,XY patients with complete AIS (CAIS) with no detectable androgen receptor site. DNA samples were digested with BamHI, EcoRI, HindIII and TaqI restriction enzymes and hybridized with three cDNA probes covering the three domains of the androgen receptor. When we had the maternal and …