Search results for "Motif"
showing 10 items of 290 documents
Binding of PTEN to specific PDZ domains contributes to PTEN protein stability and phosphorylation by microtubule-associated serine/threonine kinases
2005
The tumor suppressor phosphatase PTEN is a key regulator of cell growth and apoptosis that interacts with PDZ domains from regulatory proteins, including MAGI-1/2/3, hDlg, and MAST205. Here we identified novel PTEN-binding PDZ domains within the MAST205-related proteins, syntrophin-associated serine/threonine kinase and MAST3, characterized the regions of PTEN involved in its interaction with distinctive PDZ domains, and analyzed the functional consequences on PTEN of PDZ domain binding. Using a panel of PTEN mutations, as well as PTEN chimeras containing distinct domains of the related protein TPTE, we found that the PTP and C2 domains of PTEN do not affect PDZ domain binding and that the …
Alix protein is substrate of Ozz-E3 ligase and modulates actin remodeling in skeletal muscle
2012
Alix/AIP1 is a multifunctional adaptor protein that participates in basic cellular processes, including membrane trafficking and actin cytoskeleton assembly, by binding selectively to a variety of partner proteins. However, the mechanisms regulating Alix turnover, subcellular distribution, and function in muscle cells are unknown. We now report that Alix is expressed in skeletal muscle throughout myogenic differentiation. In myotubes, a specific pool of Alix colocalizes with Ozz, the substrate-binding component of the muscle-specific ubiquitin ligase complex Ozz-E3. We found that interaction of the two endogenous proteins in the differentiated muscle fibers changes Alix conformation and pro…
Integrin-dependent and -independent functions of astrocytic fibronectin in retinal angiogenesis
2011
Fibronectin (FN) is a major component of the extracellular matrix and functions in cell adhesion, cell spreading and cell migration. In the retina, FN is transiently expressed and assembled on astrocytes (ACs), which guide sprouting tip cells and deposit a provisional matrix for sprouting angiogenesis. The precise function of FN in retinal angiogenesis is largely unknown. Using genetic tools, we show that astrocytes are the major source of cellular FN during angiogenesis in the mouse retina. Deletion of astrocytic FN reduces radial endothelial migration during vascular plexus formation in a gene dose-dependent manner. This effect correlates with reduced VEGF receptor 2 and PI3K/AKT signalli…
A sequence motif enriched in regions bound by the Drosophila dosage compensation complex
2010
Abstract Background In Drosophila melanogaster, dosage compensation is mediated by the action of the dosage compensation complex (DCC). How the DCC recognizes the fly X chromosome is still poorly understood. Characteristic sequence signatures at all DCC binding sites have not hitherto been found. Results In this study, we compare the known binding sites of the DCC with oligonucleotide profiles that measure the specificity of the sequences of the D. melanogaster X chromosome. We show that the X chromosome regions bound by the DCC are enriched for a particular type of short, repetitive sequences. Their distribution suggests that these sequences contribute to chromosome recognition, the genera…
Modélisation, Analyse et Traitement de l'Information
2016
Mes activités de recherche s’articulent, d’une part, autour de l’instrumentation et du génie biomédical,et, d’autre part, autour du traitement et de la transmission non linéaire de l’information. Elles sebasent sur la modélisation des signaux à partir de modèles non linéaires (principalement modèles deréaction-diffusion. . . ) continus (EDP) et discrets (numériques). Dans cette partie, d’un point de vuefondamental, des phénomènes dynamiques complexes ou chaotiques sont caractérisés à travers l’analyse,la classification, la reconnaissance des motifs dans des signaux physiologiques ou issus des circuitsélectroniques. Un autre axe sur lequel je travaille concerne l’analyse et le traitement des…
Le grand débat national, une aide pour prendre des décisions locales?
2021
The Great National Debate, decided by Emmanuel Macron at the beginning of 2019 to respond to the Yellow Vests social movement, allowed the collection of citizens’ contributions on the ecological transition via an online platform. In this article, we use the corpus constituted by these contributions to identify areas where participants are asking for the development of bicycle paths and railway facilities. For this purpose, we have created a classification model to identify contributions dealing with the theme of transportation and proposed a method for extracting patterns that reflect the contributors’ proposals. We then represented these patterns on maps, using the contributors’ postal cod…
Analysis of E2F1 by clAP1
2017
The cellular inhibitor of Apoptosis 1 (cIAP1) behaves as an E3 ubiquitin ligase and has oncogenic properties. Previously, our team has shown that cIAP1 can regulate the E2F1 transcription factor activity. My research project has been focused on deepening our current knowledge on this interaction. Firstly, we characterized the E2F1-cIAP1 interaction, then we analyzed the regulation of E2F1 by cIAP1 and finally assessed the importance of the cIAP1-E2F1 interaction for the oncogenic properties of cIAP1. I have demonstrated a interaction of E2F1 with the hydrophobic pocket of the BIR3 domain of cIAP1. Moreover, I highlighted that the alpha 1 helix of the BIR3 domain is mandatory for the stabili…
Saint-Évroult-Notre-Dame-du-Bois, salle capitulaire de l’abbaye
2013
International audience
Recours en annulation. Sentence. Contrôle. Contradiction de motifs. Irrecevabilité
2000
International audience; (Cass. 1re civ., 11 mai 1999, Sté Rivers c/ Fabre, Rev. arb. 1999.810, note E. Gaillard ; Paris 26 oct. 1999, J. Patou parfumeur c/ Sté Edipar, Rev. arb. 1999.810, note E. Gaillard)
Les difficultés du motif de la profondeur charnelle chez M. Merleau-Ponty
2008
International audience