Search results for "Mussel"
showing 10 items of 136 documents
Induction of apoptosis in the blue mussel Mytilus galloprovincialis by tri-n-butyltin chloride
2001
Induction of apoptosis by tri-n-butyltin (TBT) in gill tissue of the mussel Mytilus galloprovincialis was investigated. The terminal dUTP nick-end labeling technique (TUNEL) was used to detect cells displaying DNA fragmentation within gill structures. Genomic DNA fragmentation was detected as characteristically ladder-like pattern of DNA fragments induced by single injection of different doses of TBT (1-5 microg/g) below the mantle, directly into the pallial fluid, after 24 h of incubation. DNA degradation of higher order DNA structure, as well as reduced G(0)/G(1) cell cycle region (the sub-G(1) region) was detectable after 1.5 h of TBT incubation. Presence of apoptotic cells in mussels' g…
Effect of pH, iron and aluminum on survival of early life history stages of the endangered freshwater pearl mussel, Margaritifera margaritifera
2011
Glochidium larvae and juveniles of the endangered freshwater pearl mussel Margaritifera margaritifera may be sensitive to low pH and metal exposure, but to our knowledge, no tolerance tests have been performed. Therefore, we exposed glochidia, fish-attached glochidia, and juveniles of the pearl mussel to low pH and increased iron (Fe) and aluminum (Al) by using realistic pH (6.0–4.5), Fe (0.5–2.0 mg L−1), and Al (0.25–1.0 mg L−1) levels periodically observed in this study site. Survival of glochidia decreased with decreasing pH, increasing Fe, and increasing Al, as well as with increasing Fe + Al concentration in a 72 h exposure. All glochidia died within 24 h in pH 4.5 and Fe 2.0 mg L−1. W…
Ocean Acidification studies in the Baia di Levante (Vulcano island, Italy). Advantages and disadvantages of the “in situ” approach.
2014
Years of scientific research have shown that acidification of oceans (OA) is an undisputed fact. Why is it so important to increase knowledge about OA? Because many animals and plants in the ocean have calcium carbonate skeletons or shells, and a decreasing in pH can affect their population health state and the marine-ecosystem structure. Another point of view about OA which must to be considered is that it may alter the behavior of sediment-bound metals, modifying their bioavailability and thus toxicity. The toxic free-ion concentration of metals such as copper may increase by as much as 115% in coastal waters in the next 100 years due to reduced pH. Since increasing atmospheric CO2 over t…
Bio-plastic recognition by mussels hemocytes
2023
The growing use of bio-polymers derivatives poses an increasingly pressing problem regarding their environmental sustainability. In particular, it should be still ascertained the claimed absence of direct and indirect influence on ecosystems and the health of living organisms, including humans. Our goal was about assessing the potential effects of poly-lactates and polyhydroxyalkanoates, the most widely used bio polymers classes with promising different applications for replacing conventional plastics on natural aquatic environments. We chose M. galloprovincialis as sentinel species since their extensive filter-feeding activity. When it is exposed to microparticles can bioaccumulate them in…
Application of accelerated solvent extraction followed by gel performance chromatography and high-performance liquid chromatography for the determina…
2005
Accelerated solvent extraction (ASE) has been evaluated as a fast alternative to methanolic saponification for the extraction of 12 polycyclic aromatic hydrocarbons (PAHs) from mussel tissue. Several solvent systems and different operating conditions were investigated. The mixture dichloromethane-acetone (1:1, v/v) gave the best recoveries at 125 degrees C and 1500 psi, in a total time of 10 min. No yield difference was found between freeze-drying (Fd) or drying the wet mussel with diatomaceous earth (Ded) prior to extraction. The ASE method was validated using the standard reference material SRM 2977, a freeze-dried mussel tissue with naturally present organic contaminants. The performance…
Determining seasonality of mussel collection from an early historic Inuit site, Labrador, Canada: Comparing thin-sections with high-resolution stable…
2018
International audience; Stable oxygen isotope (delta O-18) analysis of mussels (Mytilus sp.) from a 16th to mid-18th century Inuit site in southern Labrador, Canada, indicates multiple seasons of shellfish collection, and by proxy, multiple seasons of site occupation. High-resolution delta O-18 sampling of shell permits a precise season of mussel collection since the temperature and freshwater signal of the local water is retained in the shell. Live-collected specimens of Mytilus sp. obtained from Labrador, were analyzed for stable oxygen isotopes and for growth patterns. These data were used to interpret results from the archaeological shells. Growth pattern analysis was conducted to test …
Parasite transmission in aquatic ecosystems under climate change: joint effects of temperature, host behavior and elimination of parasite larvae by p…
2019
AbstractA moderate raise in temperature was suggested to enhance the impact of parasites on aquatic ecosystems. Under higher temperatures, poikilothermic animals (e.g. fish), increase their activity, which can result in a more frequent encounter with parasites. However, temperature increase may also trigger processes counteracting an increased risk of parasitic infections. For instance, removal of free-living stages of parasites by filter-feeding organisms can increase with temperature and potentially mitigate disease risk in ecosystems under climate change.In our study, we aimed to find out whether an increased infection transmission under higher temperatures can be, at least, partly compe…
Identification, cloning and environmental factors modulation of a αβ defensin from the lessepsian invasive mussel Brachidontes pharaonis (Bivalvia: M…
2015
International audience; Immunological effectors of invasive species playing a role in addressing new colonization are still poorly studied. In the present study the cDNA sequence of the defensin from a Lessepsian invasive species, the Red Sea mussel Brachidontes pharaonis, was cloned using RACE method. Defensins are a class of widely known antimicrobial peptides (AMPs), oligopeptides with a broad spectrum of targeted organisms ranging from viruses to parasites. Analysis of BpDef sequence (262 bp) revealed the presence of an ORF coding for 81 amino acids. The full-length amino acid sequence showed the highest similarity to antimicrobial peptides MGD1 and MGD2 sequence from Mytilus galloprovi…
Evaluation of the toxicity of mussels from 2 sites of the Moroccan Atlantic Coast (Jorf Lasfar and Oualidia) used as bioindicators of contamination :…
2012
Toxic substances generated by various human activities are spilled on different area of the Moroccan coast. Shellfishes can concentrate pollutants and have some adverse effects on human health through the food chain. Despite the strengthening of food safety rules, the involvement of chemical pollution of food on metabolic disorders is not known. To predict the impact of pollutants on the aquatic ecosystem and human health, the development of appropriate biomonitoring tools is required.We quantified heavy metals (Cd, Cr and Pb) in mussels (Mytilus galloprovincialis) from two sites of Moroccan Atlantic coast (industrial site Jorf Lasfar (JL) and touristic site Oualidia (OL)) due to the proxim…
Activation of MAP kinase signaling pathway in the mussel Mytilus galloprovincialis as biomarker of environmental pollution
2010
Stimulation of MAP kinase signal transduction pathway by various stressful stimuli was investigated in the marine bivalve Mytilus galloprovincialis. Analyses were performed in animals exposed in laboratory to selected pollutants and in mussels collected in winter and summer along the eastern Adriatic coast (Croatia). Effects of oxidative stress, induced by tributyltin, hydrogen peroxide and water soluble fraction of diesel fuel on the activation/phosphorylation of the three Mitogen-Activated Protein Kinases (MAPKs) p38, JNK and ERK using a newly developed ELISA procedure were evaluated. MAP kinase activation was analyzed 1 h after exposure of mussels to chemical agents, and after recovery p…