Search results for "N-3"
showing 10 items of 365 documents
Distinct Roles of Classical and Lectin Pathways of Complement in Preeclamptic Placentae
2022
Pre-eclampsia is a pregnancy complication characterized by defective vascular remodeling in maternal decidua responsible for reduced blood flow leading to functional and structural alterations in the placenta. We have investigated the contribution of the complement system to decidual vascular changes and showed that trophoblasts surrounding unremodeled vessels prevalent in preeclamptic decidua fail to express C1q that are clearly detected in cells around remodeled vessels predominant in control placenta. The critical role of C1q is supported by the finding that decidual trophoblasts of femaleC1qa-/-pregnant mice mated toC1qa+/+male mice surrounding remodeled vessels express C1q of paternal …
Biogeneration of 1-octen-3-ol by lipoxygenase and hydroperoxide lyase activities of Agaricus bisporus
2001
Abstract The biogeneration of 1-octen-3-ol by lipoxygenase and associated hydroperoxide lyase activities of a homogenate of the cultivated mushroom Agaricus bisporus was investigated using linoleic acid as a substrate. The optimal substrate and protein concentrations were 1.5 mM and 1.5 mg ml −1 , respectively. The V max value for the enzymic coupled reaction lipoxygenase–hydroperoxide lyase was 6 μg of 1-octen-3-ol ml −1 min −1 , with a K m value of 0.3×10 −3 M. The highest enzymic activity was observed in the presence of pure oxygen (22.9 μg of 1-octen-3-ol ml −1 ), with a bioconversion yield of 36%. The production of 380 μg 1-octen-3-ol per g of mushroom homogenate was demonstrated using…
CCDC 667774: Experimental Crystal Structure Determination
2008
Related Article: M.V.Veidis, L.Orola, R.Arajs|2008|Acta Crystallogr.,Sect.E:Struct.Rep.Online|64|o1062|doi:10.1107/S160053680801372X
CCDC 619384: Experimental Crystal Structure Determination
2007
Related Article: I.Kanizsai, Z.Szakonyi, R.Sillanpaa, F.Fulop|2006|Tetrahedron Lett.|47|9113|doi:10.1016/j.tetlet.2006.10.069
CCDC 884884: Experimental Crystal Structure Determination
2013
Related Article: S.Gonta,M.Utinans,G.Kirilov,S.Belyakov,I.Ivanova,M.Fleisher,V.Savenkov,E.Kirilova|2013|Spectrochim.Acta,Part A|101|325|doi:10.1016/j.saa.2012.09.104
CCDC 1891128: Experimental Crystal Structure Determination
2020
Related Article: Marcos Escolano, Marta Guerola, Javier Torres, Daniel Gaviña, Gloria Alzuet-Piña, María Sánchez-Rosello, Carlos del Pozo|2020|Chem.Commun.|56|1425|doi:10.1039/C9CC09113K
Nucleus incertus—An emerging modulatory role in arousal, stress and memory
2011
A major challenge in systems neuroscience is to determine the underlying neural circuitry and associated neurotransmitters and receptors involved in psychiatric disorders, such as anxiety and depression. A focus of many of these studies has been specific brainstem nuclei that modulate levels of arousal via their ascending monoaminergic projections (e.g. the serotonergic dorsal raphe, noradrenergic locus ceruleus and cholinergic laterodorsal tegmental nucleus). After years of relative neglect, the subject of recent studies in this context has been the GABAergic nucleus incertus,1 which is located in the midline periventricular central gray in the ‘prepontine’ hindbrain, with broad projection…
Effects of the MDM2 inhibitor Nutlin-3a on sensitivity of pancreatic cancer cells to berberine and modified berberines in the presence and absence of…
2021
Abstract Approaches to improve pancreatic cancer therapy are essential as this disease has a very bleak outcome. Approximately 80% of pancreatic cancers are pancreatic ductal adenocarcinomas (PDAC). A key regulatory gene frequently mutated (∼75%) in PDAC is the TP53 tumor suppressor gene which controls the transcription of multiple genes involved in cell cycle progression, apoptosis, cancer progression and other growth regulatory processes. The mouse double minute 2 homolog (MDM2) gene product is a nuclear-localized E3 ubiquitin ligase and negatively regulates the TP53 protein which results in its proteasomal degradation. Various MDM2 inhibitors have been isolated and examined in clinical t…
Analysis of immunosuppressive factors produced by tumorspheres in NSCLC: Prognostic value of galectin-3 in adenocarcinoma
2019
Abstract Background Cancer stem cells (CSCs) are targets poorly recognized by the immune surveillance system given that they induce an immunosuppressive microenvironment. The aim of this work is to study the interactions between CSCs and the immune microenvironment in NSCLC. Methods Tumor cells from 8 resected NSCLC patients and 12 cell lines were established as tumorspheres enriched in CSCs and as monolayers. The gene expression of IL-4, IL-10, IL6, IL8 and LGALS-3 was analyzed by RTqPCR. Results were validated at a protein level by a sensitivity bead-based multiplex immunoassay using the Millipore kit for Luminex 100/200. The prognostic value of these factors in silico was determined in a…
MMP-13 stimulates osteoclast differentiation and activation in tumour breast bone metastases
2011
INTRODUCTION: The increased bone degradation in osteolytic metastases depends on stimulation of mature osteoclasts and on continuous differentiation of new pre-osteoclasts. Metalloproteinases (MMP)-13 is expressed in a broad range of primary malignant tumours and it is emerging as a novel biomarker. Recent data suggest a direct role of MMP-13 in dissolving bone matrix complementing the activity of MMP-9 and other enzymes. Tumour-microenvironment interactions alter gene expression in malignant breast tumour cells promoting osteolytic bone metastasis. Gene expression profiles revealed that MMP-13 was among the up-regulated genes in tumour-bone interface and its abrogation reduced bone erosion…