Search results for "NAK"
showing 10 items of 469 documents
Targeting Carcinoembryonic Antigen with DNA Vaccination: On-Target Adverse Events Link with Immunologic and Clinical Outcomes.
2015
Abstract Purpose: We have clinically evaluated a DNA fusion vaccine to target the HLA-A*0201–binding peptide CAP-1 from carcinoembryonic antigen (CEA605–613) linked to an immunostimulatory domain (DOM) from fragment C of tetanus toxin. Experimental Design: Twenty-seven patients with CEA-expressing carcinomas were recruited: 15 patients with measurable disease (arm-I) and 12 patients without radiological evidence of disease (arm-II). Six intramuscular vaccinations of naked DNA (1 mg/dose) were administered up to week 12. Clinical and immunologic follow-up was up to week 64 or clinical/radiological disease. Results: DOM-specific immune responses demonstrated successful vaccine delivery. All p…
Therapeutic application of T cell receptor mimic peptides or cDNA in the treatment of T cell-mediated skin diseases
2000
An 8-amino acid peptide encoding a sequence of the transmembrane region of the T cell receptor alpha chain (TCR-alpha) was shown to inhibit T cell function by preventing functional assembly of the T cell receptor (mimic peptide). To avoid systemic immunosuppression by peptide application in vivo, we used a topical application of the peptide. In the system of murine contact sensitivity, topical application of the peptide inhibited the elicitation of contact sensitivity following application of a contact allergen in sensitized animals. Alternatively, when naked DNA encoding the peptide sequence was injected into skin before application of a contact allergen to sensitized animals, local immuno…
Optical recognition and removal of Hg(II) using a new self-chemosensor based on a modified amino-functionalized Al-MOF
2017
Abstract We developed a simple self-chemical optical sensor for the monitoring and removal of ultra-trace levels of Hg(II) from aqueous media. The development of this sensor was based on the covalent attachment of amino-functionalized aluminum-based MOF particles with ninhydrin. The new sensor is densely coated with a chelating ligand to permit an ultra-fast, selective, pH-dependent visualization for removal of Hg(II) with detection limit (LOD∼0.494 μg L−1). Monitoring was accomplished via both a colorimetric signal visible to the naked eye as well as UV–vis absorption spectroscopy. Digital image-based colorimetric analysis has also used as a semi-quantitative analysis for determination the…
New optical paper sensor for in situ measurement of hydrogen sulphide in waters and atmospheres
2016
A novel and low-cost colorimetric sensor for the determination of hydrogen sulphide in environmental samples has been developed. This sensor is based on the immobilization of the reagent N,N-Dimethyl-p-phenylenediamine and FeCl3 in paper support, in which the H2S is adsorbed in order to give rise to the formation of methylene blue as reaction product. The sensor has been applied to determine H2S in water and air samples. Two different sampling systems for H2S caption from the air have been assayed: active and passive sampling. The analytical properties of the different systems have been obtained and compared. The analytical signals, corresponding to the methylene blue, have been obtained me…
3-Formyl-BODIPY Phenylhydrazone as a Chromo-Fluorogenic Probe for Selective Detection of NO2 (g)
2016
Anew colorimetric and fluorogenic probe,based on a3-formyl boron dipyrromethene (BODIPY)phe-nylhydrazone, for the sensitive and selectivedetectionNO2(g) has been prepared. The probeinsolution experi-ences aremarkablehypsochromic shift of its absorptionand fluorescence emission bands in the presence gaseousNO2(g), leading to limits of detectio noffew ppb. Theprobe also works in the solid phase, adsorbed on filterpaper strips, or chemically immobilized on the surfaceofsilica nanop articles, with limitsofdetection to the nakedeye of about 0.5 ppm.
Atgriešanās līmeņa aprēķināšana ekstremāliem nokrišņiem Latvijā
2015
Darbā tika aplūkota ekstremālo nokrišņu atgriešanās līmeņa aprēķināšana ar statistiskās modelēšanas palīdzību. Tas tika veikts ar divām dažādam metodēm, kas ietver sadalījuma piemeklēšanu datiem. Pirmā metode piemeklē sadalījumu gada maksimālām novērotām vērtībām (bloku maksimumu metode), otrā — datiem, kas pārsniedz noteikto slieksni (maksimumu virs sliekšņa metode). Lai pārbaudītu vai atrastais sadalījums atbilst datiem, tika veikti atbilstošie statistiskie testi. Tika salīdzināti abu metožu rezultāti novērotiem nokrišņu datiem Latvijā 1961.-2010. periodā. Iegūtie rezultāti ir svarīgi, jo tiem ir liela nozīme lēmumu pieņemšanas procesos, kas ir saistīti ar vadības, plānošanas, projektēšan…
Aikariippuvan Diracin yhtälön numeerisesta ratkaisemisesta
2017
Tässä opinnäytetyössä tarkastellaan Diracin yhtälön numeerista ratkaisemista sekä tähän liittyviä haasteita. Diracin yhtälö on keskeinen kvanttifysiikan ilmiöitä kuvaava yhtälö, jonka ratkaisemiseen on esitetty kirjallisuudessa useita erilaisia menetelmiä. Tässä opinnäytetyössä keskitytään tarkastelemaan menetelmää, jossa yhtälön ratkaisemiseen sovelletaan operaattorinjakoa ja karakteristista menetelmää. Yhtälön ratkaiseminen vaatii paljon aikaa ja laskentaresursseja, joten laskennan tehostaminen rinnakkaislaskennan keinoin on välttämätöntä. In this paper the numerical solution of the Dirac equation is studied along with its challenges. The Dirac equation is a central equation describing th…
Variation in predator species abundance can cause variable selection pressure on warning signaling prey
2012
Predation pressure is expected to drive visual warning signals to evolve toward conspicuousness. However, coloration of defended species varies tremendously and can at certain instances be considered as more camouflaged rather than conspicuous. Recent theoretical studies suggest that the variation in signal conspicuousness can be caused by variation (within or between species) in predators' willingness to attack defended prey or by the broadness of the predators' signal generalization. If some of the predator species are capable of coping with the secondary defenses of their prey, selection can favor reduced prey signal conspicuousness via reduced detectability or recognition. In this study…
Stability of PEI–DNA and DOTAP–DNA complexes: effect of alkaline pH, heparin and serum
2001
Abstract DNA complexes formed with nonviral vectors such as polyethylenimine (PEI) or 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) are widely used in gene therapy. These complexes prevent the interaction of DNA with the fluorescent probes usually employed to quantify DNA. We thus studied the procedures for DNA quantification from DNA complexes as well as their stability in the presence of DNase or mouse, rat and human sera. Release of the DNA from its complexes was accomplished by increasing the pH of the medium (from 7.3 to 13.4) or by adding heparin. The stability against degradation was tested in vitro, by incubating the complexes at 37°C in the presence of DNase I and sera from the …
Physicochemical characterization of the fifth (C5), sixth (C6), seventh (C7), eighth (C8) and ninth (C9) component of guinea pig complement.
1971
A physicochemical characterization of the purified guinea pig complement components C5 to C9 is given. For this purpose the sedimentation rate, the diffusion coefficient, the molecular weight and the isoelectric point were determined and compared with the values already known for the guinea pig and human complement system. For the determination of the physicochemical parameters gel filtration on Sephadex G-200, ultracentrifugation applying a sucrose density gradient and thin-layer isoelectric focusing were used. By comparing the values of the human and guinea pig complement a remarkable similarity is shown.