Search results for "NAS"
showing 10 items of 12064 documents
150 years of the discovery of the Cambrian Lagerstätte of Murero (Cadenas Ibéricas, NE Spain)
2013
El yacimiento paleontológico del Cámbrico inferior-medio de Murero (Cadenas Ibéricas, NE de España) es una localidad clásica del Cámbrico europeo. Fue dado a conocer a la ciencia por Edouard de Verneuil en 1862, celebrándose en 2012 el 150 aniversario de su descubrimiento. Es un siglo y medio de trabajos geológicos y de logros puestos al servicio del conocimiento del Período Cámbrico. Murero es un Lagerstätte excepcional porque coexisten en él grupos esqueléticos y de cuerpo blando a lo largo de un registro estratigráfico continuo que abarca unos 8 millones de años, dividido en 14 zonas de trilobites. En 1997, este yacimiento paleontológico fue el primero de España en obtener de la Administ…
Fiesta y urbanismo. Valencia en los siglos XVI y XVII
2017
El objetivo principal de la presente tesis doctoral se centra en el análisis de la influencia que ejerció la fiesta como elemento transformador de la ciudad de Valencia durante los siglos XVI y XVII. Nuestra investigación se configuró a través de un estudio bicéfalo en el que por un lado tratamos de profundizar en el urbanismo valenciano y por otro, en el mundo festivo. Basándonos en algunas investigaciones que abarcaban el periodo medieval valenciano, como las regestas de María Milagros Cárcel Ortí, en las que trataban de exponer la importancia de la relación de estos dos campos de estudio, observamos que había la posibilidad de una continuación de este tipo de transformación urbana durant…
Implicación de miRNAs en la toxicidad mediada por expansiones de repeticiones CTG en Distrofia Miotónica
2015
La distrofia miotónica tipo 1 (DM1) es una enfermedad neuromuscular causa por la expansión del triplete CTG en la región 3’ no codificante del gen DMPK. Las expansiones CUG en los transcritos DMPK mutantes forman una estructura en horquilla que secuestra diferentes factores nucleares provocando su falta de función parcial y la desregulación de la expresión génica a diferentes niveles. La mayoría de los defectos en la expresión génica se han reproducido en animales modelo para la enfermedad que expresan transcritos con repeticiones CUG de manera independiente a DMPK. En este trabajo nos propusimos analizar si un grupo de reguladores de la expresión génica está afectado por la toxicidad media…
A Contribution to the analysis of the first Spanish public-private partnership in primary and specialized maternity care: a social network analysis a…
2021
The La Ribera University Hospital (Alzira, Valencia) was the first experience of public-private partnership in the Spanish National Health System. In addition to the need to coordinate two levels of health care (primary/specialised), two models of human resource management (public/private) had to be coordinated. To illustrate this complexity, the group of midwives in the health area has been taken, analysing their relational patterns through the methodological approach of Social Network Analysis. In terms of results, out of a population of 31 midwives, those of the public model occupy peripheral positions and cooperate poorly, both internally and externally. However, those of the private mo…
Into the wild of long non-coding RNA in gastrointestinal stromal tumors (GISTs) to explore new prognostic/predictive biomarkers
2015
Background: Long Non-coding RNAs (lncRNA) are emerging as essential regulators of genetic and epigenetic networks, and their deregulation may underlie complex diseases, such as carcinogenesis. Several studies have described lncRNAs alterations in patients with solid tumors. In particular, in Gastrointestinal Stromal Tumors (GIST), upregulation of HOTAIR has been associated with aggressiveness, metastasis, and poor patients’ survival. In order to gain more detailed insight on the molecular role of lncRNAs in GIST, we analyzed in vivo the expression levels of lncRNAs H19 and MALAT1 in surgically resected patients. Material and Methods: The expression of the lnc-RNAs H19 and MALAT1 was evaluat…
2019
Parthenolide (PT) is a sesquiterpene lactone isolated from Tanacetum parthenium. In this study, PT showed varying cytotoxic effects against different solid tumor cell lines. HCT116 (p53+/+) colon carcinoma cells and their parental HCT116 knockout p53 (p53-/-) cell lines showed a resistance degree of 2.36. On the other hand, wild-type U87.MG cells or cells transfected with a deletion-activated EGFR cDNA (U87.MGΔEGFR) exhibited slight sensitivity toward PT. Multidrug-resistant MDA-MB-231-BCRP cells were even more sensitive toward PT than sensitive MDA-MB-231-pcDNA cells with a resistance degree of 0.07 (collateral sensitivity). To the best of our knowledge, hypersensitivity (collateral sensit…
2014
Oligodendrocytes are the myelinating glial cells of the central nervous system. In the course of brain development, oligodendrocyte precursor cells migrate, scan the environment and differentiate into mature oligodendrocytes with multiple cellular processes which recognize and ensheath neuronal axons. During differentiation, oligodendrocytes undergo dramatic morphological changes requiring cytoskeletal rearrangements which need to be tightly regulated. The non-receptor tyrosine kinase Fyn plays a central role in oligodendrocyte differentiation and myelination. In order to improve our understanding of the role of oligodendroglial Fyn kinase, we have identified Fyn targets in these cells. Pur…
Different modes of activating phosphofructokinase, a key regulatory enzyme of glycolysis, in working vertebrate muscle
2002
Glycolytic flux in white muscle can be increased several-hundredfold by exercise. Phosphofructokinase (PFK; EC 2.7.1.11) is a key, regulatory enzyme of glycolysis, but how its activity in muscle is controlled is not fully, understood. In order not to neglect integrative aspects of metabolic regulation, we have studied in frogs (Rana temporaria) a physiological form of muscle work (swimming) that can be triggered like a reflex. We analysed swimming to fatigue in well rested frogs, recovery from exercise, and repeated exercise after 2 h of recovery. At various times, gastrocnemius muscles were tested for glycolytic intermediates and effectors of PFK. All metabolites responded similarly to the…
Tenascin in denervated human muscle
1996
Tenascin is a large oligomeric glycoprotein of the extracellular matrix. Its location is limited in innervated muscle tissues. We investigated immunohistologically, using two monoclonal antibodies (mab) against Tenascin, biopsied denervated human muscle of children and adults. Tenascin was present in the interstitial space among denervated muscle fibres. Accumulation of Tenascin in denervated adult muscle tissue was frequent, accumulation in denervated muscle tissue of children was sparse and weak. The two antibodies reacted correspondingly. Tenascin was not only found in the vicinity of atrophic muscle fibres, but also close to normally sized fibres, suggesting an early stage of denervatio…
Synthesis, Anti-Inflammatory Activity, and in Vitro Antitumor Effect of a Novel Class of Cyclooxygenase Inhibitors: 4-(Aryloyl)phenyl Methyl Sulfones
2010
Following our previous research on anti-inflammatory drugs (NSAIDs), we report on the design and synthesis of 4-(aryloyl)phenyl methyl sulfones. These substances were characterized for their capacity to inhibit cyclooxygenase (COX-1 and COX-2) isoenzymes. Molecular modeling studies showed that the methylsulfone group of these compounds was inserted deep in the pocket of the human COX-2 binding site, in an orientation that precludes hydrogen bonding with Arg120, Ser353, and Tyr355 through their oxygen atoms. The N-arylindole 33 was the most potent inhibitor of COX-2 and also the most selective (COX-1/COX-2 IC(50) ratio was 262). The indole derivative 33 was further tested in vivo for its ant…