Search results for "NBO"
showing 10 items of 266 documents
SiRNA-mediated selective inhibition of mutant keratin mRNAs responsible for the skin disorder pachyonychia congenita.
2006
RNA interference offers a novel approach for treating genetic disorders including the rare monogenic skin disorder pachyonychia congenita (PC). PC is caused by mutations in keratin 6a (K6a), K6b, K16, and K17 genes, including small deletions and single nucleotide changes. Transfection experiments of a fusion gene consisting of K6a and a yellow fluorescent reporter (YFP) resulted in normal keratin filament formation in transfected cells as assayed by fluorescence microscopy. Similar constructs containing a single nucleotide change (N171K) or a three-nucleotide deletion (N171del) showed keratin aggregate formation. Mutant-specific small inhibitory RNAs (siRNAs) effectively targeted these site…
CCDC 222871: Experimental Crystal Structure Determination
2003
Related Article: K.Ejsmont, J.Zaleski, A.Sporzynski, M.Lewandowski|2003|Acta Crystallogr.,Sect.E:Struct.Rep.Online|59|o1324|doi:10.1107/S1600536803017999
CCDC 255863: Experimental Crystal Structure Determination
2005
Related Article: B.Zarychta, K.Ejsmont, M.Bujak, J.Zaleski, A.Sporzynski, A.Miskiewicz, A.Strutynska, J.Serwatowski|2004|Acta Crystallogr.,Sect.E:Struct.Rep.Online|60|o1925|doi:10.1107/S1600536804023293
Effects of progressive decrease of feeding frequency and re-feeding on production parameters, stomach capacity and muscle nutritional value in rainbo…
2020
Abstract Feeds and feeding constitute the major part of costs in intensive aquaculture. Any action to reduce feeding costs without negatively affecting fish production parameters and flesh quality would improve profitability of farming. Therefore, we studied the effects of feeding frequency on production parameters, stomach capacity and nutritional value of muscle in juvenile rainbow trout (Oncorhynchus mykiss) in an experiment with two stages. First, during the nine-week “starvation period” we fed rainbow trout (initial weight c. 40 g) with four different feeding protocols in an attempt to adapt the fish to a progressive decrease in the number of feeding days. During the second stage, a fo…
Effects of weekend starvation and the duration of daily feeding on production parameters of rainbow trout Oncorhynchus mykiss
2021
Abstract It would often be practical to starve the cultivated fishes over the weekends, e.g. to save in labour costs. We evaluated the possibility that domesticated juvenile rainbow trout (Oncorhynchus mykiss) could compensate for the lost growth of the 2-day weekend starvation by either hyperphagic response and/or by lower feed conversion ratio, compared to the fish fed every day in an 8-week experiment. Rainbow trout (initial weight c. 30 g, temperature 16 °C) starved during the weekends were able to increase feed intake during the weekdays clearly above the intake of the control fish after the first two weekends, also seen as an increase of the compensation coefficients over the last fou…
Global properties of generalized Ornstein–Uhlenbeck operators on Lp(RN,RN) with more than linearly growing coefficients
2009
AbstractWe show that the realization Ap of the elliptic operator Au=div(Q∇u)+F⋅∇u+Vu in Lp(RN,RN), p∈[1,+∞[, generates a strongly continuous semigroup, and we determine its domain D(Ap)={u∈W2,p(RN,RN):F⋅∇u+Vu∈Lp(RN,RN)} if 1<p<+∞. The diffusion coefficients Q=(qij) are uniformly elliptic and bounded together with their first-order derivatives, the drift coefficients F can grow as |x|log|x|, and V can grow logarithmically. Our approach relies on the Monniaux–Prüss theorem on the sum of noncommuting operators. We also prove Lp–Lq estimates and, under somewhat stronger assumptions, we establish pointwise gradient estimates and smoothing of the semigroup in the spaces Wα,p(RN,RN), α∈[0,1], wher…
Identification and dose dependency of ibuprofen biliary metabolites in rainbow trout
2012
Abstract The biotransformation of the anti-inflammatory drug ibuprofen (IBF) was studied by exposing rainbow trout ( Oncorhynchus mykiss ) to IBF via intraperitoneal (i.p.) injection, and via water at four (0.17, 1.9, 13 and 145 μg L −1 ) exposure levels for 4 d. Following exposure, the bile was collected and analyzed by LC–MS/MS methods. The identification of the formed metabolites in i.p. injected fish bile was based on the exact mass determinations by a time-of-flight mass analyzer (Q–TOF–MS) and on the studies of fragments and fragmentation patterns of precursor ions by ion trap mass analyzer (IT-MS). In addition to unmetabolized IBF, several phase I and phase II metabolites were found …
Host specificity of Argulus coregoni (Crustacea: Branchiura) increases at maturation.
2007
SUMMARYWe tested the hypothesis that host specificity in ectoparasites does not depend exclusively on the features of the host but also on surrounding habitats, using 2 fish ectoparasites, Argulus coregoni and A. foliaceus (Crustacea: Branchiura), occurring sympatrically in Finnish lakes. Although these parasites are considered to be of low specificity, we found that the larger of the 2 species, A. coregoni developed a pronounced preference for salmonid hosts at the beginning of maturation (defined by the presence of copulating specimens). Argulus foliaceus infects a much wider range of fish hosts. We showed that specialization of A. coregoni on salmonids does not necessarily result from in…
Efficacy of emamectin benzoate in the control of Argulus coregoni (Crustacea: Branchiura) on rainbow trout Oncorhynchus mykiss.
2004
Efficacy of in-feed treatment with emamectin benzoate (Slice) for the control of ectoparasitic Argulus coregoni on rainbow trout Oncorhynchus mykiss was tested under laboratory and field conditions. In both experiments fish were fed with fish feed to deliver a therapeutic dose of 0 (control) or 50 microg emamectin benzoate kg(-1) d(-1) (treatment) for a period of 7 d. After 3 d of challenge with A. coregoni in the laboratory, the infestation level in treated fish was lower than that observed in the controls (p0.001). Efficacy of 100% against newly hatched A. coregoni metanauplii and adults and 80% against juveniles was observed. In the field, trial medication was undertaken at 2 sections on…
Species differences, influence of dose and application on biotransformation of phenol in fish.
1983
1. The metabolism of phenol in goldfish (Carassius auratus), rainbow trout (Salmo gairdneri) and goldenorfe ide (Leuciscus ideus melanotus) was compared. All three species produced phenyl sulphate, quinol sulphate and phenyl glucuronide. 2. The dose dependence of metabolism of phenol in goldfish was investigated. When the concn. of phenol was increased from 0.2 to 2 mg/l medium, that glucuronylated increased from 7 to 16% and that sulphated decreased from 63 to 47%. 3. The influence of mode of exposure on metabolism of phenol in goldfish was examined. Dietary exposure and uptake from medium was compared with i.p. injection. There was less metabolism in the order: dietary exposure greater th…