Search results for "NEB"

showing 10 items of 543 documents

Characterization of a putative extracellular matrix protein from the beetle Tenebrio molitor: hormonal regulation during metamorphosis.

2004

0949-944X (Print) Journal Article Research Support, Non-U.S. Gov't; We used differential display to isolate epidermis cDNAs corresponding to juvenile-hormone analog-regulated mRNA from the beetle Tenebrio molitor. One of them encodes a putative extracellular matrix (ECM) protein, named Tenebrin. Indeed, the deduced protein sequence contains ECM typical features like the presence of a signal peptide, internal repeats, a RGD tripeptide sequence motif known to bind integrins and von Willebrand factor type c domains involved in protein-protein interactions. Northern blot analysis reveals a single transcript of about 11 kb with an expression pattern correlated to 20-hydroxyecdysone fluctuations …

Signal peptideIntegrinMolecular Sequence DataCycloheximideBiologyExtracellular matrixchemistry.chemical_compoundExtracellular Matrix Proteins/*genetics/metabolismGeneticsAnimalsDevelopmentalHormones/physiologyNorthern blotAmino Acid SequenceTenebrioTenebrio/*geneticsDifferential displayMessenger RNAExtracellular Matrix ProteinsMetamorphosisMetamorphosis BiologicalGene Expression Regulation DevelopmentalBiological/*physiologyHormoneschemistryBiochemistryGene Expression RegulationJuvenile hormonebiology.proteinDevelopmental BiologyDevelopment genes and evolution
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Characterization of two new cuticular genes specifically expressed during the post-ecdysial molting period in Tenebrio molitor

1998

Abstract In a previous study, we have isolated a cDNA, TM-ACP17 , coding for a post-ecdysial adult protein of Tenebrio molitor . After screening of a genomic library with TM-ACP17 , we report isolation and sequencing of TM-ACP17 gene and a new gene, TM-LPCP29 , coding for a larval–pupal protein. These two genes exhibit a common sequence of 15 nucleotides and a characteristic of most cuticular protein genes so far described: an intron interrupting the signal peptide. The deduced aa sequence of TM-LPCP29 exhibits a high percentage of Ala (26.5%) and Val (17.5%) and is highly hydrophobic. In the N-terminal part, the motif VAAPV is repeated ten times. Numerous histidine residues are present in …

Signal peptideMolecular Sequence DataGene ExpressionGenes InsectMoltingBiologyComplementary DNAGeneticsAnimalsGenomic libraryAmino Acid SequenceRNA MessengerTenebrioGeneHistidineMessenger RNAGenomeBase SequenceSequence Homology Amino AcidPupaIntronGene Expression Regulation DevelopmentalDNASequence Analysis DNAGeneral MedicineMolecular biologyGenesBiochemistryLarvaInsect ProteinsMoultingGene
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Characterization of a cDNA clone encoding a glycine-rich cuticular protein of Tenebrio molitor: developmental expression and effect of a juvenile hor…

1992

0962-1075 (Print) Journal Article; The complete sequence of a cDNA clone, isolated from epidermal mRNA of Tenebrio molitor using a monoclonal antibody raised against an adult-specific cuticular antigen only present in the hard cuticle, was obtained after primer extension at the 5' end. From this cDNA sequence, the deduced protein encompasses 199 amino acids (including a signal peptide) with a total molecular weight of 20.7 kDa. The protein exhibits a bipartite structure: glycine-rich region located in its NH2-terminal part and a carboxy-terminal domain sharing homologies with other cuticular proteins of Orthoptera, Diptera and Lepidoptera. In-situ hybridization analysis shows that the corre…

Signal peptideanimal structuresMethoprene/*pharmacologyCuticleMolecular Sequence DataGlycineBiologyPrimer extensionBiological/drug effects/geneticsComplete sequenceComplementary DNAGeneticsAnimalsAmino Acid SequenceCloning MolecularTenebrioTenebrio/drug effects/*genetics/growth & developmentMolecular BiologyEpidermis/chemistry/growth & developmentProteins/drug effects/*genetics/isolation & purificationchemistry.chemical_classificationMessenger RNABase SequenceMetamorphosisfungiMetamorphosis BiologicalProteinsMolecularSequence Analysis DNADNAMethopreneMolecular biologyAmino acidGlycine/*genetics/metabolismchemistryInsect ScienceJuvenile hormoneInsect ProteinsEpidermisSequence AnalysisCloning
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Renaissance Itineraries between Nebrodi and Peloritani: Architectural Heritage, History and Technology

2022

Within the general framework of a research Project of Relevant National Interest (PRIN 2017), entitled “The Renaissance in Southern Italy and the Islands: Cultural Heritage and Technology”, the research unit based at Palermo University focused on the inner area that includes Nebrodi and Peloritani mountains, as a quite unexplored and stimulating environment in the specific field of investigation. The scientific relevance of the investigation on “Renaissance itineraries” in this territory, for a deeper understanding of the overall cultural phenomenon in the island, guided this choice, as well as the opportunity to create cultural networks among small local communities, encouraging their sust…

Southern Renaissance Cultural itineraries Architectural heritage Nebrodi PeloritaniSettore ICAR/18 - Storia Dell'Architettura
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CCDC 1519437: Experimental Crystal Structure Determination

2017

Related Article: Arto Valkonen, Giuseppe M. Lombardo, Kari Rissanen, Francesco Punzo, Massimo Cametti|2017|Dalton Trans.|46|5240|doi:10.1039/C6DT04773D

Space GroupCrystallography(NN-dimethylformamide)-(11'-(12-phenylenebis(iminomethyl))bis(naphthalen-2-olato))-dioxo-uranium NN-dimethylformamide solvateCrystal SystemCrystal StructureCell ParametersExperimental 3D Coordinates
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CCDC 814707: Experimental Crystal Structure Determination

2011

Related Article: N.Khiri, E.Bertrand, M.-J.Ondel-Eymin, Y.Rousselin, J.Bayardon, P.D.Harvey, S.Juge|2010|Organometallics|29|3622|doi:10.1021/om100520u

Space GroupCrystallography(S~p~)-(-)-(((1S2R)-2-(diphenylphosphinitoborane)-1-methyl-2-phenylethyl)(methyl)amino-25262728-tetrapropoxycalix(4)arene)phosphaneboraneCrystal SystemCrystal StructureCell ParametersExperimental 3D Coordinates
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CCDC 2151292: Experimental Crystal Structure Determination

2022

Related Article: Sara Fuertes, Lorenzo Mardegan, Ignacio Martínez, Silvia Ventura, Irene Ara, Daniel Tordera, Henk J. Bolink, Violeta Sicilia|2022|J.Mater.Chem.C|10|15491|doi:10.1039/D2TC02539F

Space GroupCrystallography(methylenebis(diphenylphosphine))-(2-(3-methylimidazol-2-ylidene-1-yl)-5-(9H-carbazol-9-yl)phenyl)-platinum hexafluorophosphate dichloromethane solvateCrystal SystemCrystal StructureCell ParametersExperimental 3D Coordinates
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CCDC 1473786: Experimental Crystal Structure Determination

2017

Related Article: Ahibur Rahaman, Carolina Gimbert-Suriñach, Arne Ficks, Graham E. Ball, Mohan Bhadbhade, Matti Haukka, Lee Higham, Ebbe Nordlander, Stephen B. Colbran|2017|Dalton Trans.|46|3207|doi:10.1039/C6DT01494A

Space GroupCrystallography(mu-(12-phenylenebis(methylene))bis(((ferrocenyl)methyl)phosphino))-hexacarbonyl-di-ironCrystal SystemCrystal StructureCell ParametersExperimental 3D Coordinates
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CCDC 2171090: Experimental Crystal Structure Determination

2023

Related Article: J. Mikko Rautiainen, Maryna Green, Minna Mähönen, Jani O. Moilanen, Manu Lahtinen, Arto Valkonen|2023|Cryst.Growth Des.|23|2361|doi:10.1021/acs.cgd.2c01351

Space GroupCrystallography11'-[13-phenylenebis(methylene)]bis(3-iodopyridin-1-ium) dibromide hemikis(3-iodopyridine)Crystal SystemCrystal StructureCell ParametersExperimental 3D Coordinates
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CCDC 726494: Experimental Crystal Structure Determination

2009

Related Article: B.R.D.Nayagam, S.R.Jebas, C.Daisy, D.Schollmeyer|2009|Acta Crystallogr.,Sect.E:Struct.Rep.Online|65|o918|doi:10.1107/S1600536809010782

Space GroupCrystallography11'-[2356-Tetramethyl-p-phenylenebis(methyleneoxy)]di-1H-benzotriazoleCrystal SystemCrystal StructureCell ParametersExperimental 3D Coordinates
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