Search results for "NEMATODE"
showing 10 items of 94 documents
Species-specific polymerase chain reaction primers for simple detection of Bursaphelenchus fraudulentus (Nematoda: Parasitaphelenchidae)
2010
Nematology , 2010, Vol. 12(1), 157-160 Short communication Species-specific polymerase chain reaction primers for simple detection of Bursaphelenchus fraudulentus (Nematoda: Parasitaphelenchidae) Anna F I L I P I A K 1 , ∗ , Agata J AKUBOW SKA 1 , 2 and Marek T OMALAK 1 Bursaphelenchus fraudulentus Ruhm, 1956 (Nema- toda: Parasitaphelenchidae) is a tree-inhabiting species frequently found in Europe (Ruhm, 1956; Schauer-Blume & Sturhan, 1989; Tomalak, 2004; Carletti et al ., 2005) and Asia (Braasch et al ., 2001). It is primarily found in dy- ing or dead deciduous trees (Ruhm, 1956; Schauer-Blume & Sturhan, 1989), although occasionally reported from conifers (Braasch et al ., 2001). Within t…
Engineering Bacteria to Form a Biofilm and Induce Clumping in Caenorhabditis elegans
2014
Bacteria are needed for a vast range of biotechnological processes, which they carry out either as pure cultures or in association with other bacteria and/or fungi. The potential of bacteria as biofactories is hampered, though, by their limited mobility in solid or semisolid media such as agricultural or domestic waste. This work represents an attempt toward overcoming this limitation by associating bacterial biotechnological properties with the transport ability of the nematode Caenorhabditis elegans. We report here biofilm formation on C. elegans by engineered Escherichia coli expressing a Xhenorhabdus nematophila adhesion operon and induction of nematode social feeding behavior (clumping…
Parasite specificity in Podarcis bocagei and P. carbonelli (Lacertidae) from NW Portugal: a host-parasite history
2015
Host parasite specificity is related to several factors including the type of life cycle of the parasite, the habitat occupied by both parasites and hosts, and the food habits of the hosts. In this work, gastrointestinal helminths in two congeneric lacertid lizards, Podarcis bocagei and Podarcis carbonelli living in sintopy, have been analysed. Out of four helminth species found, three of them, the trematode Brachylaima sp., the cestode Oochoristica gallica and the nematode Skrjabinelazia hoffmanni, have indirect life cycles while the nematode Spauligodon carbonelli has a direct life cycle. Heteroxenous helminths showed low host specificity whereas S. carbonelli may be placed at an intermed…
Regulation of Phenotypic Switching and Heterogeneity in Photorhabdus luminescens Cell Populations.
2019
Phenotypic heterogeneity in bacterial cell populations allows genetically identical organisms to different behavior under similar environmental conditions. The Gram-negative bacterium Photorhabdus luminescens is an excellent organism to study phenotypic heterogeneity since their life cycle involves a symbiotic interaction with soil nematodes as well as a pathogenic association with insect larvae. Phenotypic heterogeneity is highly distinct in P. luminescens. The bacteria exist in two phenotypic forms that differ in various morphologic and phenotypic traits and are therefore distinguished as primary (1°) and secondary (2°) cells. The 1 cells are bioluminescent, pigmented, produce several sec…
New findings of Setaria tundra and Setaria cervi in the red deer (Cervus elaphus) in Poland
2019
AbstractOur study aimed at examining the phylogenetic position of the newly-found Setaria nematodes obtained from the red deer (Cervus elaphus) based on sequences of the mitochondrial cytochrome c oxidase subunit 1 (COX-1). Alignment and phylogenetic analyses, as well as SEM microscopic analysis, revealed the presence of two Setaria species: S. cervi and S. tundra. Setaria tundra was noted in only one individual, a calf of the red deer, while S. cervi was observed in three stages, two hinds and one calf of the red deer. According to our knowledge, it is the first case of S. cervi in the red deer in Poland confirmed in molecular studies and also the first case of S. tundra infection in the r…
Proteomic analysis of the pinworm Syphacia muris (Nematoda: Oxyuridae), a parasite of laboratory rats
2012
Syphacia muris (Nematoda: Oxyuridae) is a ubiquitous nematode that commonly infects rats in the laboratory which can interfere in the development of biological assays. The somatic extract of S. muris adults collected from infected rats was investigated using a proteomic approach. A shot-gun liquid chromatography/tandem mass spectrometry procedure was used. We used the MASCOT search engine (Matrix-Science) and ProteinPilot software v2.0 (Applied Biosystems) for the database search. A total of 359 proteins were accurately identified from the worms. The largest protein families consisted of metabolic enzymes and those involved in the nucleic metabolism and cell cycle. Proteins of transmembrane…
A quantitative survey of the benthic meiofauna in the Gulf Of Riga (eastern Baltic Sea), with special reference to the structure of nematode assembla…
1998
Abstract The Gulf of Riga is relatively shallow and considered to be one of the most eutrophied areas in the Baltic Sea with an annual primary production estimated to 290 gCm-2 yr-1. Meiofauna sampling was carried out at 30 stations homogeneously spread over the Gulf of Riga, varying in depth and sediment structure. The average meiofauna abundance was 4800 ind/10 cm2, with a maximum of 17000 ind/10 cm2. Harpacticoid copepods were abundant all over the Gulf, averaging 425 ind/10 cm2. The highest number was found close to the mouth of the river Daugava, up to 1400 ind/10 cm2. Harpacticoids were significantly more abundant in sediments with medium organic content (loss on ignition (LOI) 5–10%)…
In vivo Antimicrobial Activity Assessment of a Cauliflower By-Product Extract Against Salmonella Typhimurium
2020
The main objective of this work was to study the antimicrobial effect of a cauliflower by-product infusion into an affordable in vivo model (Caenorhabditis elegans). The infusion demonstrated some protective effect on non-infected and infected worms with Salmonella Typhimurium as indicated by higher survival percentile values (75, 50, 25, and 5% percentiles) as compared with those from worms unexposed to the infusion. The antimicrobial effect of the infusion was evaluated on Salmonella intestinal colonization of infected worms (24, 48, and 96 h post-infection). At 96 h post-infection, the concentration of Salmonella was reduced around 2 log cycles in infected cauliflower treated group (p < …
Pristionchus sp. (Rhabditida: Diplogastridae) from Italian populations of Anoplophora chinensis Forster (Coleoptera: Cerambycidae).
2009
Priostonchus sp. n. (Rhabditida: Diplogastridae) is described from a population of Citrus Longhorn Beetles. Alive adults of this Cerambycid were collected from Acer saccharinum trees at Assago and Parabiago (Villastanza), two localities near Milan (Italy). The natural occurrence of the entomogenous nematode in the beetle was not frequent and widespread in the study area. Preliminary laboratory infectivity tests highlight the potential parasitic nature of the nematode. However, it was not successfully maintained in storage and culture. The diplogasterid Pristionchus sp. has rather complicate systematic thus a biomolecular analysis based on DNA is necessary for more specific identification an…
Confronto tra la nematofauna delle monoculture di mais e quella dei sistemi erbacei
2023
Gli studi sugli effetti dell'agricoltura sulla biodiversità del suolo sono diventati molto importanti, al fine di proteggere la salute dell'ambiente e migliorare la qualità dell'agricoltura. Le comunità di nematodi hanno dimostrato di offrire un potente strumento ecologico per valutare gli effetti delle pratiche agricole sul suolo. La ricerca è stata condotta in una monocoltura di mais (della durata di 20 anni) e in un ecosistema erbaceo seminaturale, dominato da Festuca spp. e Trifolium spp.. Ciascun sistema è stato sottoposto a due diverse pratiche di gestione delle colture (input alto (H) e basso (L)). L'obiettivo era quindi quello di studiare gli effetti di due diversi ecosistemi e dive…