6533b855fe1ef96bd12b1264
RESEARCH PRODUCT
Engineering Bacteria to Form a Biofilm and Induce Clumping in Caenorhabditis elegans
Marina MañasCristina VilanovaPedro Dorado-moralesManuel PorcarJuli PeretóAlejandro TorresLucas J MoralesJessica De LomaAlba IglesiasGuillermo ZafrillaAlba CormanAlejandro ValeroSamuel Miravet-verdeAntonio Ruizsubject
OperonBiomedical EngineeringBioengineeringBiologymedicine.disease_causeBiochemistry Genetics and Molecular Biology (miscellaneous)Biotechnological processMicrobiologyRNA interferenceEscherichia colimedicineAnimalsCaenorhabditis elegansCaenorhabditis elegans ProteinsSymbiosisEscherichia coliCaenorhabditis elegansBiofilmFeeding BehaviorGeneral Medicinebiology.organism_classificationNematodeBiofilmsRNA InterferenceSynthetic BiologyBacteriadescription
Bacteria are needed for a vast range of biotechnological processes, which they carry out either as pure cultures or in association with other bacteria and/or fungi. The potential of bacteria as biofactories is hampered, though, by their limited mobility in solid or semisolid media such as agricultural or domestic waste. This work represents an attempt toward overcoming this limitation by associating bacterial biotechnological properties with the transport ability of the nematode Caenorhabditis elegans. We report here biofilm formation on C. elegans by engineered Escherichia coli expressing a Xhenorhabdus nematophila adhesion operon and induction of nematode social feeding behavior (clumping) through an E. coli-mediated iRNA blocking on the expression of FLP-21, a neuropeptide involved in worm solitary behavior.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-06-17 | ACS Synthetic Biology |