Search results for "NOE"

De novo production of six key grape aroma monoterpenes by a geraniol synthase-engineered S. cerevisiae wine strain

2015

[Background] Monoterpenes are important contributors to grape and wine aroma. Moreover, certain monoterpenes have been shown to display health benefits with antimicrobial, anti-inflammatory, anticancer or hypotensive properties amongst others. The aim of this study was to construct self-aromatizing wine yeasts to overproduce de novo these plant metabolites in wines.

Emmy Noether’s Long Struggle to Habilitate in Göttingen

2020

Doctoral degrees have a long prehistory, but the modern Ph.D. first arose as part of an educational reform launched at the German universities. Over the course of the nineteenth century, this degree came to be awarded not merely to those who displayed a command of established knowledge in an academic field.

Diving into Math with Emmy Noether

2020

Some 100 years ago a notice appeared in the journal of the German Mathematical Society that read: “Dr. Emmy Noether has habilitated as a lecturer in mathematics at Gottingen University.” This quiet announcement was actually the resounding final chord in a long struggle that went on for four years and only ended on June 4, 1919, when Noether joined the Gottingen faculty.

Expression and Secretion of Barley Cysteine Endopeptidase B and Cellobiohydrolase I in Trichoderma reesei.

2006

Localization of expression and secretion of a heterologous barley cysteine endopeptidase (EPB) and the homologous main cellobiohydrolase I (CBHI) in a Trichoderma reesei transformant expressing both proteins were studied. The transformant was grown on solid medium with Avicel cellulose and lactose to induce the cbh1 promoter for the synthesis of the native CBHI and the recombinant barley protein linked to a cbh1 expression cassette. Differences in localization of expression between the two proteins were clearly indicated by in situ hybridization, indirect immunofluorescence, and immunoelectron microscopy. In young hyphae, native-size recombinant epb mRNA was localized to apical compartments…

Steam and gas emission rates from La Soufrière of Guadeloupe (Antilles arc): implications for the magmatic supply degassing during unrest

2015

Since its last magmatic eruption in 1530 AD, La Soufrière andesitic volcano in Guadeloupe has displayed intense hydrothermal activity and six phreatic eruptive crises (the last of which, in 1976-1977, with 73000 evacuees). Here we report on the first direct quantification of gas plume emissions from La Soufrière summit vents, which gradually intensified during the past 20 years. Gas fluxes were determined in 2006 then 2012 [1] by measuring the horizontal and vertical distribution of volcanic gas concentrations in the air-diluted plume, the composition of the hot fumarolic fluid at exit (108°C), and scaling to the speed of plume transport (in situ measurements and FLIR imaging). We first dem…

Isolation of carcinoembryonic antigen N-terminal domains (N-A1) from soluble aggregates

2011

Abstract Carcinoembryonic antigen (CEA) was identified as a prominent tumor-associated antigen in human colorectal cancer and it is still intensively investigated. However, its physiological role remains unclear. The CEA molecule is composed of seven highly hydrophobic, immunoglobulin-like domains, six of which contain a single disulphide bridge. The production of recombinant protein containing Ig-like domains in bacterial expression systems often results in partial degradation or insolubility due to aggregation hampering the analysis of their native structure and function. Here, we present a new method of expression and purification of CEA N-terminal domains (N-A1) fused to MBP in Escheric…

Tourisme Sportif et Identités : Les pratiquants de canoë en Zambie

2013

Cet article repose sur une enquête sur les usages sociaux du canoë en Zambie. Il a pour objectif de décrire les caractéristiques des relations sociales qui se nouent entre étrangers et « populations locales ». Si l’organisation de l’activité recouvre une visée touristique pour nombre de pays africains, touristes et habitants entrent peu en interaction en dehors de la pratique des loisirs. Pour étudier l’identité, il s’agit de caractériser la genèse du tour en canoë afin de saisir l’émergence de modes d’appréhension de l’Autre dans le cadre du loisir en Zambie. La singularité des relations sociales entre populations occidentales et africaines pose la question de la différence culturelle, au …

In vitro synthesis of factor B of the alternative pathway of complement activation by mouse peritoneal macrophages

1976

Factor B of the alternative pathway of complement activation was shown to be synthesized and secreted by unstimulated mouse peritoneal macrophages. The activity of B in the culture supernatants from macrophage monolayers was detected by consumption of C3 in reaction mixtures containing supernatant and guinea pig factors C3, D and insoluble C3b. Using a monospecific antiserum, factor B in concentrated culture supernatants was shown by immunodiffusion and immunoelectrophoresis to be identical to factor B in mouse plasma and to form a characteristic complex with cobra venom factor in the presence of D. A steady rate of factor B secretion was observed for 4 days providing the medium was changed…

Extramammary Paget's disease arising in a mature cystic teratoma of the ovary.

1999

The architecture ofMourera fluviatilis(Podostemaceae): developmental morphology of inflorescences, flowers, and seedlings

1999

Mourera fluviatilis from northern South America is a spectacular member of the Podostemaceae (river-weeds). Its racemelike inflorescences are up to 64 cm long and have 40‐90 flowers arranged in two opposite rows. Inflorescence development starts with the initiation of a double-sheathed (dithecous) bract in a terminal position. All lateral bracts (again dithecous) are initiated in basipetal order along the two flanks of the inflorescence. Each gap between two neighboring bracts contains a single flower. The flowers are bisexual, each with a whorl of 16‐20 ligulate tepals and 14‐40 stamens, which are arranged in one or two whorls. Floral development starts with the formation of a girdling pri…