Search results for "NSP4"

showing 3 items of 3 documents

Canine-Origin G3P[3] Rotavirus Strain in Child with Acute Gastroenteritis

2007

Infection by an animal-like strain of rotavirus (PA260/97) was diagnosed in a child with gastroenteritis in Palermo, Italy, in 1997. Sequence analysis of VP7, VP4, VP6, and NSP4 genes showed resemblance to a G3P[3] canine strain identified in Italy in 1996. Dogs are a potential source of human viral pathogens.

RotavirusSettore MED/07 - Microbiologia E Microbiologia ClinicaDisease reservoircanine rotavirusEpidemiologyvirusesSequence Homologylcsh:Medicinemedicine.disease_causePolymerase Chain Reactionlaw.inventionfluids and secretionslawZoonosesRotavirushuman rotavirusGenotypePotential sourceDog DiseasesRotavirusG3P[3] VP7 VP4PhylogenyPolymerase chain reactionStrain (chemistry)Dispatchvirus diseasesGastroenteritisVP7Infectious DiseasesChild PreschoolVP4Acute DiseaseVP6Microbiology (medical)GenotypeNSP4Sequence analysisBiologyRotavirus Infectionslcsh:Infectious and parasitic diseasesMicrobiologyDogsSpecies SpecificitymedicineAnimalsHumanslcsh:RC109-216G3P[3] genotypeDisease Reservoirslcsh:RAcute gastroenteritisVirologyEmerging Infectious Diseases
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Genetic characterization of G3 rotaviruses detected in Italian children in the years 1993–2005

2009

International audience; In recent years an apparent increase in the frequency of detection of G3P[8] rotaviruses has been observed worldwide. Similarly, in Italy G3P[8] strains have been detected sporadically and in a scattered fashion over 20 years, whereas in 2003 and 2005 G3P[8] rotavirus activity increased markedly. By analysis of the VP7, VP4, VP6 and NSP4 genes of a selection of G3P[8] rotaviruses detected between 1993 and 2005, a remarkable sequence conservation was observed in the VP7, VP4 and VP6 genes. By converse, after 2002 the Italian G3P[8] strains were found to possess unique mutations in significant regions of the NSP4 protein.

RotavirusSettore MED/07 - Microbiologia E Microbiologia ClinicaSettore MED/17 - Malattie InfettiveSequence analysisNSP4virusesMolecular Sequence DataReoviridaeViral Nonstructural ProteinsBiologymedicine.disease_causeRotavirus InfectionsVirusFeces03 medical and health sciencesfluids and secretionsViral geneticsPhylogeneticsVirologyRotavirusGenotypemedicineHumansAmino Acid SequenceAntigens ViralGenePhylogenyGlycoproteinsToxins Biological030304 developmental biology0303 health sciencesSequence Analysis RNA030306 microbiologyInfant NewbornInfantvirus diseasesbiology.organism_classificationVirologyInfectious DiseasesItalyChild PreschoolRNA ViralMedicineCapsid Proteinssequence analysirotavirus G3P[8]gastroenteriti
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Assignment of the group A rotavirus NSP4 gene into genotypes using a hemi-nested multiplex PCR assay: a rapid and reproducible assay for strain surve…

2009

The rotavirus non-structural protein NSP4 has been implicated in a number of biological functions during the rotavirus cellular cycle and pathogenesis, and has been addressed as a target for vaccine development. The NSP4 gene has been classified into six genotypes (A–F). A semi-nested triplex PCR was developed for genotyping the major human NSP4 genotypes (A–C), which are common in human rotavirus strains but are also shared among most mammalian rotavirus strains. A total of 192 previously characterized human strains representing numerous G and P type specificities (such as G1P[8], G1P[4], G2P[4], G3P[3], G3P[8], G3P[9], G4P[6], G4P[8], G6P[4], G6P[9], G6P[14], G8P[10], G8P[14], G9P[8], G9P…

Rotavirus NSP4Microbiology (medical)RotavirusSettore MED/07 - Microbiologia E Microbiologia ClinicaDNA ComplementaryGenotypeSwinevirusesReassortmentMolecular Sequence DataReoviridaeBiologyViral Nonstructural Proteinsmedicine.disease_causeMicrobiologylaw.inventionFecesDogsSpecies SpecificitylawRotavirusGenotypeMultiplex polymerase chain reactionmedicineAnimalsHumansGenotypingPolymerase chain reactionPhylogenyDNA PrimersGlycoproteinsToxins BiologicalElectrophoresis Agar GelBase SequenceReverse Transcriptase Polymerase Chain ReactionReproducibility of ResultsGeneral MedicineHaplorhinibiology.organism_classificationVirologyMolecular biologyCatsRNA ViralCattleNested polymerase chain reactionJournal of medical microbiology
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