Search results for "Native"

Aceruloplasminemia: a case report

2008

Hereditary aceruloplasminemia is a rare autosomal recessive disease, firstly identified by Miyajima et al. in Japan in 1987 [1]. The disease is caused by the absence of an a2glycoprotein, the ceruloplasmin (Cp), a copper-containing ferroxidase, mainly synthesized in hepatocytes and widely expressed, including the central nervous system, which catalyses the oxidation of ferrous to ferric iron, a change required for release of iron to plasma transferrin [2]. It is hypothesized that in reticuloendothelial (RE) cells and hepatocytes Cp cooperates to export iron with the iron exporter protein ferroportin 1 (FPN1) [3]. As a consequence, Cp deficiency results in iron deposition in the liver, pancr…

Artesunate – An anti-malarial natural product derivative for cancer therapy

2015

Characterization of hOGG1 Promoter Structure, Expression During Cell Cycle and Overexpression in Mammalian Cells

2001

Oxygen radicals are produced in all cells either by the normal cellular metabolism or by the exposure to external mutagens. The reactive oxygen species (ROS) generated can induce DNA damage. Among the principal lesions found in DNA due to ROS is an oxidized form of guanine, 8-oxo-7,8-dihydroguanine (8-oxoG). The biological relevance of this lesion has been unveiled by the study of Escherichia colt and Saccharomyces cerevisiae genes involved in the neutralization of the mutagenic effects of 8-oxoG (Cabrera et al., 1988; Nghiem et al., 1988; Radicella et al., 1988; van der Kemp et al., 1996). These genes fpg and mutY for E. colt and OGG1 for yeast, code for DNA glycosylases. Inactivation of a…

Role of epigenetic factors in the selection of the alternative splicing isoforms of human

2017

Mutation-driven activation of KRAS is crucial to cancer development. The human gene yields four mRNA splicing isoforms, 4A and 4B being translated to protein. Their different properties and oncogenic potential have been studied, but the mechanisms deciding the ratio 4A/4B are not known. To address this issue, the expression of the four KRAS isoforms was determined in 9 human colorectal cancer cell lines. HCT116 and SW48 were further selected because they present the highest difference in the ratio 4A/4B (twice as much in HCT116 than in SW48). Chromatin structure was analysed at the exon 4A, characteristic of isoform 4A, at its intronic borders and at the two flanking exons. The low nucleoso…

Circular RNAs in Sepsis: Biogenesis, Function, and Clinical Significance

2020

Sepsis is a life-threatening condition that occurs when the body responds to an infection that damages it is own tissues. The major problem in sepsis is rapid, vital status deterioration in patients, which can progress to septic shock with multiple organ failure if not properly treated. As there are no specific treatments, early diagnosis is mandatory to reduce high mortality. Despite more than 170 different biomarkers being postulated, early sepsis diagnosis and prognosis remain a challenge for clinicians. Recent findings propose that circular RNAs (circRNAs) may play a prominent role in regulating the patients’ immune system against different pathogens, including bacteria and viruses. Mou…

Le combat de Victor Schoelcher contre la légalité de l'esclavage : l'abolition de 1848

2009

How has the French State been able to maintain slavery for more than fifty years after the Déclaration des droits de l'homme et du citoyen of 1789? The slave was considered, in a legal scope, not as a person but as a « movable », he was considered as an object that could be bought, sold, mutilated and tortured! Abolished for the first time in 1794, reinstated in 1802 by Napoleon Bonaparte, slavery would definitively disappear in France only in 1848, thanks to Victor Schoelcher's (1804-1893) decisive action. This great humanist, by a tireless fight, managed to impose an immediate abolition – and not a progressive one as in Britain – showing that if this institution was certainly legal, it wa…

Victor Schoelcher, abolitionniste et républicain : approche juridique et politique de l'oeuvre d'un fondateur de la République

2000

The aim of this judicial and political analysis is to reveal Victor Schoelcher's decisive influence in the fight for equal rights and for the republic, in the French mainland as well as in its colonies. His well known fight against slavery was just a part of his humanist defense of the republican principles : Liberty, Equality, Fraternity. Schoelcher truly was a founder of the republic, taking part in the barricade insurrections against the 1851 coup and fighting against any kind of slavery or absolutism. His work addressed many aspects of our society, among which : - Slavery and the colonies (legal status of slaves, emancipated slaves and of the colonies) - Rights and liberties of men and …

Correlation between Cognition and Balance among Middle-Aged and Older Adults Observed through a Tai Chi Intervention program

2020

Background: Age-associated decline in cognition and balance may cause severe ability loss for daily living activities among middle-aged and older adults. The relationship between cognition and balance in this aging population remains to be explored. Objective: The present study Is exploratory in nature and aimed to examine the relationship between balance (both static and dynamic components) and global cognitive function among middle-aged and older adults through Tai Chi (TC) practice as a research avenue. Methods: A short-term (12 weeks) intervention of TC was conducted among middle-aged and older adults in the community setting. Global cognitive function (using the Chinese version of the …

Effects of cold acclimation and dsRNA injections on Gs1l gene splicing in Drosophila montana

2017

Abstract Alternative splicing, in which one gene produce multiple transcripts, may influence how adaptive genes respond to specific environments. A newly produced transcriptome of Drosophila montana shows the Gs1-like (Gs1l) gene to express multiple splice variants and to be down regulated in cold acclimated flies with increased cold tolerance. Gs1l’s effect on cold tolerance was further tested by injecting cold acclimated and non-acclimated flies from two distantly located northern and southern fly populations with double stranded RNA (dsRNA) targeting Gs1l. While both populations had similar cold acclimation responses, dsRNA injections only effected the northern population. The nature of …

Genetic and Chemical Modifiers Of A CUG Toxicity Model in Drosophila

2007

Non-coding CUG repeat expansions interfere with the activity of human Muscleblind-like (MBNL) proteins contributing to myotonic dystrophy 1 (DM1). To understand this toxic RNA gain-of-function mechanism we developed a Drosophila model expressing 60 pure and 480 interrupted CUG repeats in the context of a non-translatable RNA. These flies reproduced aspects of the DM1 pathology, most notably nuclear accumulation of CUG transcripts, muscle degeneration, splicing misregulation, and diminished Muscleblind function in vivo. Reduced Muscleblind activity was evident from the sensitivity of CUG-induced phenotypes to a decrease in muscleblind genetic dosage and rescue by MBNL1 expression, and furthe…