Search results for "Nucleic acid sequence"
showing 5 items of 105 documents
Chloroplastic glutamine synthetase from Brassica napus.
1993
Glutamine synthetase from roots of Brassica napus. Nucleotide sequence of a cytosolic isoform.
1994
Complete Nucleotide Sequence of a Hemoglobin Gene Cluster from the Midge Chironomus thummi piger
1991
The aquatic larvae of non-biting midges (Chironomidae, Diptera) contain a variety of Hb proteins in their hemolymph that enable them to survive in an anoxic environment (1). In Chironomus thummi thummi, 12 different Hb variants have been identified and their amino acid sequences determined (2). Based on these primary structures, the evolutionary relationships between the five monomeric and the s e v e n dimeric Hb proteins have been deduced (2). The two groups are thought to have evolved in two different lineages which separated more than 255 million years ago.
Sequence of a new tRNALeu(U∗AA) from brewer's yeast
1991
The nucleotide sequence of a new tRNA(Leu)(anticodon U*AA) from Saccharomyces cerevisiae which could recognize exclusively the UUA codon has been determined. Its primary structure is: pGGAGGGUUGm2GCac4CGAGDGmGDCDAAGGCm2(2)GGCAGACmUU*AAm1GA++ + psi CUGUUGGACGGUUGUCCGm5CGCGAGT psi CGm1A(orA)ACCUCGCAUCCUUCACCA. This tRNA has a large extraloop and contains 15 modified nucleotides. So far it is the third isoacceptor tRNA for leucine in yeast. It has 61% homology with tRNA(Leu)(anticodon m5CAA) and 63% homology with tRNA(Leu)(anticodon UAG), the two other known yeast tRNAs(Leu).
Contact sites of peptide-oligoribonucleotide cross-links identified by a combination of peptide and nucleotide sequencing with MALDI MS.
1997
We have investigated peptide–oligoribonucleotide complexes isolated from cross-linked Escherichia coli 30S ribosomal subunits in order to identify the contact sites of these complexes at the molecular level. For this purpose, reversed-phase (RP) HPLC-purified peptide–oligoribonucleotide complexes were submitted to N-terminal amino acid sequencing in order to determine the cross-linked peptide moiety and were analyzed using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for calculation of the nucleotide composition of the cross-linked complex. Subsequently, for nucleotide sequence information the complexes were partially hydrolyzed or treated with exonucleases and a…