Search results for "OIT"

showing 10 items of 9012 documents

Estrogenic regulation of skeletal muscle proteome : a study of premenopausal women and postmenopausal MZ cotwins discordant for hormonal therapy

2017

Female middle age is characterized by a decline in skeletal muscle mass and performance, predisposing women to sarcopenia, functional limitations, and metabolic dysfunction as they age. Menopausal loss of ovarian function leading to low circulating level of 17b-estradiol has been suggested as a contributing factor to aging-related muscle deterioration. However, the underlying molecular mechanisms remain largely unknown and thus far androgens have been considered as a major anabolic hormone for skeletal muscle. We utilized muscle samples from 24 pre- and postmenopausal women to establish proteome-wide profiles, associated with the difference in age (30–34 years old vs. 54– 62 years old), men…

0301 basic medicineAgingnaisetlabel‐free protein quantitationProteomeAnabolismvaihdevuodetmedicine.medical_treatmentTwinsmenopausenano‐LC‐HD‐MSElihakset0302 clinical medicineSTRENGTHBRAIN315 Sport and fitness sciencesta315luustoINHIBITORHormone replacement therapy (menopause)ta3142MITOCHONDRIAL BIOGENESISMiddle AgedPostmenopauseMenopauseREPLACEMENThormone replacement therapyEditorialmedicine.anatomical_structurehormonihoitoHormonal therapyOriginal ArticleFemalemuscleswomenAdultestrogeenitnano-LC-HD-MSEEXPRESSIONmedicine.medical_specialtyBiologyestrogenic regulation03 medical and health sciencesmitochondrial functionInternal medicinemedicineHumansMuscle Skeletallabel-free protein quantitationmuscle proteomeAgedSkeletal muscleEstrogenslabel-free proteinquantitationOriginal ArticlesCell Biologyfunctional annotationmedicine.diseaseMiddle ageMONOZYGOTIC TWIN PAIRS030104 developmental biologyEndocrinologyPremenopauselihasmassaSarcopeniaCELLS3111 BiomedicineEnergy Metabolismfemale muscle030217 neurology & neurosurgeryskeletal musclesHormone
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Autophagy is induced by resistance exercise in young men, but unfolded protein response is induced regardless of age.

2017

AIM Autophagy and unfolded protein response (UPR) appear to be important for skeletal muscle homoeostasis and may be altered by exercise. Our aim was to investigate the effects of resistance exercise and training on indicators of UPR and autophagy in healthy untrained young men (n = 12, 27 ± 4 years) and older men (n = 8, 61 ± 6 years) as well as in resistance-trained individuals (n = 15, 25 ± 5 years). METHODS Indicators of autophagy and UPR were investigated from the muscle biopsies after a single resistance exercise bout and after 21 weeks of resistance training. RESULTS Lipidated LC3II as an indicator of autophagosome content increased at 48 hours post-resistance exercise (P < .05) and …

0301 basic medicineAutophagosomeAdultMalemedicine.medical_specialtyTime FactorsPhysiologyta3111Endoplasmic Reticulum03 medical and health sciencesYoung Adult0302 clinical medicineSex FactorsInternal medicinemedicineAutophagyHumansMuscle Strengthta315Muscle SkeletalsolufysiologiaAgedbusiness.industryEndoplasmic reticulumAutophagyResistance trainingAge FactorsAutophagosomesSkeletal muscleResistance TrainingMiddle AgedOxidative Stress030104 developmental biologyEndocrinologymedicine.anatomical_structureAgeingUnfolded protein responseUnfolded Protein ResponsevoimaharjoittelubusinessMicrotubule-Associated Proteins030217 neurology & neurosurgeryHomeostasisMuscle ContractionActa physiologica (Oxford, England)
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The cytoprotective protein MANF promotes neuronal survival independently from its role as a GRP78 cofactor

2021

Mesencephalic astrocyte-derived neurotrophic factor (MANF) is an endoplasmic reticulum (ER)-stress-regulated protein exhibiting cytoprotective properties through a poorly understood mechanism in various in vitro and in vivo models of neuronal and non-neuronal damage. Although initially characterized as a secreted neurotrophic factor for midbrain dopamine neurons, MANF has recently gained more interest for its intracellular role in regulating the ER homeostasis, including serving as a cofactor of the chaperone glucose-regulated protein 78 (GRP78). We aimed for a better understanding of the neuroprotective mechanisms of MANF. Here we show for the first time that MANF promotes the survival of …

0301 basic medicineBiFC bimolecular fluorescence complementationMST microscale thermophoresisPDIA1 protein disulfide isomerase family A member 1ApoptosisNEUROTROPHIC FACTOR MANFEndoplasmic ReticulumBiochemistryprotein-protein interactionMiceBimolecular fluorescence complementationUPR unfolded protein responseENDOPLASMIC-RETICULUM STRESSMesencephalonNeurotrophic factorsInsulin-Secreting CellsProtein Interaction MappingBINDINGCOMPREHENSIVE RESOURCEATF6unfolded protein response (UPR)PDIA6 protein disulfide isomerase family A member 6PPIs protein-protein interactionsEndoplasmic Reticulum Chaperone BiPHeat-Shock ProteinsNPTN neuroplastinbiologyChemistryapoptosisunfolded protein responsedopamine neurons3. Good healthCell biologyGDNF glial cell line–derived neurotrophic factorIRE1-ALPHASBD substrate-binding domainendoplasmic reticulum stressMANF mesencephalic astrocyte-derived neurotrophic factorTm tunicamycinneuroprotectionResearch ArticleProtein BindingSignal TransductionGRP78Protein Disulfide-Isomerase FamilyCell SurvivalTH tyrosine hydroxylasePrimary Cell CultureSCG superior cervical ganglionProtein Disulfide-IsomerasesIRE1 inositol-requiring enzyme 1ER-STRESSER endoplasmic reticulum03 medical and health sciencesohjelmoitunut solukuolemaC-MANF C-terminal domain of MANFCSPs chemical shift perturbationsAnimalsHumansHSP70 Heat-Shock ProteinsNerve Growth FactorsNBD nucleotide-binding domainNMR nuclear magnetic resonanceMolecular Biology030102 biochemistry & molecular biologyBIPATF6Dopaminergic NeuronsGene Expression ProfilingBinding proteinneuronal cell deathDISSOCIATIONCell BiologyNEI nucleotide exchange inhibitorEmbryo MammalianadenosiinitrifosfaattiATPhermosolutmesencephalic astrocyte-derived neurotrophic factorprotein–protein interactionPERK protein kinase RNA-like ER kinaseHEK293 Cells030104 developmental biologyGene Expression RegulationChaperone (protein)Tg thapsigarginbiology.proteinUnfolded protein responseAP-MS affinity purification mass spectrometry1182 Biochemistry cell and molecular biologyGFP-SH SH-tagged GFPendoplasmic reticulum stress (ER stress)DA dopaminemesencephalic astrocyte-derived neurotrophic factor (MANF)proteiinitNeuroplastin
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Five-year follow-up results of aerobic and impact training on bone mineral density in early breast cancer patients

2021

Summary A 12-month exercise program reversibly prevented hip bone loss in premenopausal women with early breast cancer. The bone-protective effect was maintained for 2 years after the end of the program but was lost thereafter. Purpose Breast cancer survivors are at an increased risk for osteoporosis and fracture. This 5-year follow-up of a randomized impact exercise intervention trial evaluated the maintenance of training effects on bone among breast cancer patients. Methods Five hundred seventy-three early breast cancer patients aged 35–68 years and treated with adjuvant therapy were allocated into a 12-month exercise program or a control group. Four hundred forty-four patients (77%) were…

0301 basic medicineBone densityEndocrinology Diabetes and MetabolismOsteoporosisphysical activityliikuntaweight-bearing impact aerobic exerciseMetabolic equivalent0302 clinical medicineAbsorptiometry PhotonBreast cancerBone DensityMedicineharjoitteluSURVIVORSRISKBone mineraltrainingrintasyöpäFemur Neckbone densityCHEMOTHERAPYMiddle Aged3. Good healthmedicine.anatomical_structurePOSTMENOPAUSAL WOMENTRIALFemaleOriginal ArticleHEALTHfyysinen aktiivisuusAdultmedicine.medical_specialtyBODY-COMPOSITIONgovernment.form_of_governmentosteoporoosi3122 CancersluuntiheysEXERCISE030209 endocrinology & metabolismBreast NeoplasmsMASSStep aerobics03 medical and health sciencesBreast cancerbreast cancerInternal medicineAdjuvant therapyHumansTrainingFemoral neckAgedbusiness.industryPhysical activitymedicine.diseaseaerobinen harjoitteluosteoporosisgovernmentOsteoporosisWeight-bearing impact aerobic exercise030101 anatomy & morphologyPHYSICAL PERFORMANCEbusinessFollow-Up Studies
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Homozygous Resistance to Thyroid Hormone β: Can Combined Antithyroid Drug and Triiodothyroacetic Acid Treatment Prevent Cardiac Failure?

2017

Resistance to thyroid hormone β (RTHβ) due to homozygous THRB defects is exceptionally rare, with only five kindreds reported worldwide. Cardiac dysfunction, which can be life-threatening, is recognized in the disorder. Here we describe the clinical, metabolic, ophthalmic, and cardiac findings in a 9-year-old boy harboring a biallelic THRB mutation (R243Q), along with biochemical, physiologic, and cardiac responses to carbimazole and triiodothyroacetic acid (TRIAC) therapy. The patient exhibits recognized features (goiter, nonsuppressed thyroid-stimulating hormone levels, upper respiratory tract infections, hyperactivity, low body mass index) of heterozygous RTHβ, with additional characteri…

0301 basic medicineCardiac function curvemedicine.medical_specialtyendocrine systemGoiterendocrine system diseasesEndocrinology Diabetes and Metabolism030209 endocrinology & metabolismCase ReportsCardiovascularthyroidresistance to thyroid hormonehomozygous THRB mutation03 medical and health sciences0302 clinical medicineClinical ResearchInternal medicinemedicine2.1 Biological and endogenous factorsDecompensation2. Zero hungercardiac thyrotoxicosisbusiness.industryThyroidDilated cardiomyopathymedicine.disease3. Good health030104 developmental biologyEndocrinologymedicine.anatomical_structureCarbimazoleHeart Disease6.1 PharmaceuticalsBasal metabolic ratebusinessHormonemedicine.drug
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Chondroprotective effects of the combination chondroitin sulfate-glucosamine in a model of osteoarthritis induced by anterior cruciate ligament trans…

2016

[EN] Context: The efficacy of the combination chondroitin sulfate-glucosamine (CS-GlcN) in the treatment of knee osteoarthritis (OA) has been suggested in recent clinical studies. In vitro reports have also suggested anti-inflammatory and anti-resorptive effects of this combination. Objective: The aim of this study was to characterize the effects of CS-GlcN on joint degradation in vivo including the assessment of inflammation and bone metabolism in a model of OA. Materials and methods: We have used the OA model induced by anterior cruciate ligament transection (ACLT) in ovariectomised rats. CS-GlcN was administered daily (oral gavage) from week 0 until week 12 after ovariectomy at the dose …

0301 basic medicineCartilage Articularmedicine.medical_specialtyAnterior cruciate ligamentOvariectomyType II collagenOsteoarthritisProtective AgentsBone and BonesBone remodeling03 medical and health scienceschemistry.chemical_compound0302 clinical medicineOsteoprotegerinGlucosamineInternal medicineOsteoarthritisMedicineAnimalsChondroitin sulfateAnterior cruciate ligament transectionAnterior Cruciate LigamentRats Wistar030203 arthritis & rheumatologyPharmacologyGlucosaminebusiness.industryCartilageAnterior Cruciate Ligament InjuriesChondroitin SulfatesGeneral MedicineX-Ray MicrotomographyOsteoarthritis Kneemedicine.diseaseChondroitin sulfate-glucosamine Ovariectomised ratscarbohydrates (lipids)Disease Models Animal030104 developmental biologymedicine.anatomical_structureEndocrinologychemistryDrug Therapy CombinationFemaleJointsInflammation MediatorsbusinessBiomarkersModel
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NG2/CSPG4 and progranulin in the posttraumatic glial scar.

2018

Traumatic injury of the central nervous system is one of the leading causes of death and disability in young adults. Failure of regeneration is caused by autonomous neuronal obstacles and by formation of the glial scar, which is essential to seal the injury but also constitutes a barrier for regrowing axons. The scar center is highly inflammatory and populated by NG2+ glia, whereas astrocytes form the sealing border and trap regrowing axons, suggesting that the non-permissive environment of activated astrocytes and extracellular matrix components is one of the reasons for the regenerative failure. Particularly, secreted chondroitin-sulfate proteoglycans, CSPGs, of the lectican family hinder…

0301 basic medicineCentral nervous systemPerlecanCell CommunicationBiologyGlial scarExtracellular matrix03 medical and health scienceschemistry.chemical_compoundCicatrix0302 clinical medicineProgranulinsmedicineLecticanAnimalsHumansMolecular BiologyMicrogliaReceptors NotchMembrane ProteinsCell biology030104 developmental biologymedicine.anatomical_structurenervous systemchemistryChondroitin Sulfate ProteoglycansChondroitin sulfate proteoglycanBrain InjuriesImmunologybiology.proteinSynaptic signalingNeuroglia030217 neurology & neurosurgeryHeparan Sulfate ProteoglycansSignal TransductionMatrix biology : journal of the International Society for Matrix Biology
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Serratus anterior contraction during resisted arm extension (GravityFit) assessed by MRI

2019

Background: Scapular stabilization is a common focus of shoulder rehabilitation. Objective: Examine contraction of serratus anterior during a bilateral arm extension exercise with axial compression using an exercise device (GravityFit) by magnetic resonance imaging (MRI). Methods: MRI was performed under two conditions: rest and static arm extension with axial compression. Load was set at 20% of age, sex and weight estimated bench press one-repetition maximum. A T2-weighted sequence was used to collect 14 axial images of the upper thoracic spine and shoulder bilaterally. Mean muscle length and thickness were calculated for the whole muscle and in equidistant subregions of the muscle in its …

0301 basic medicineContraction (grammar)PhysiologymusclelihaksetBench presslcsh:PhysiologyfysioterapiarehabilitationUpper thoracic spine03 medical and health sciences0302 clinical medicineShoulder rehabilitationPhysiology (medical)Axial compressionupper extremityMedicinephysical therapyTransversus abdominisphysiotherapyOriginal Research030222 orthopedicslcsh:QP1-981medicine.diagnostic_testexercisebusiness.industrymagneettikuvausMagnetic resonance imagingAnatomyTrunk030104 developmental biologykuntoutusvoimaharjoittelubusiness
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Comparison of hemolytic activity of the intermediate subunit of Entamoeba histolytica and Entamoeba dispar lectins.

2017

Galactose and N-acetyl-D-galactosamine-inhibitable lectin of Entamoeba histolytica has roles in pathogenicity and induction of protective immunity in rodent models of amoebiasis. Recently, the intermediate subunit of the lectin, Igl1, of E. histolytica has been shown to have hemolytic activity. However, the corresponding lectin is also expressed in a non-virulent species, Entamoeba dispar, and another subunit, Igl2, is expressed in the protozoa. Therefore, in this study, we compared the activities of Igl1 and Igl2 subunits from E. histolytica and E. dispar using various regions of recombinant Igl proteins expressed in Escherichia coli. The recombinant E. dispar Igl proteins had comparable h…

0301 basic medicineErythrocytesTime Factorslcsh:MedicineProtein Sequencingmedicine.disease_causePathology and Laboratory MedicineBiochemistrylaw.inventionEntamoebafluids and secretionslawLectinsMedicine and Health SciencesRecombinant Protein Purificationlcsh:ScienceProtozoansMultidisciplinarybiologyPseudomonas AeruginosaRecombinant ProteinsBacterial PathogensMedical MicrobiologyRecombinant DNAPathogensResearch ArticleProtein PurificationProtein subunitDisparResearch and Analysis MethodsReal-Time Polymerase Chain ReactionMicrobiologyHemolysisMicrobiologyEntamoeba Histolytica03 medical and health sciencesEntamoeba histolyticaPseudomonasParasite Groupsparasitic diseasesmedicineAnimalsTrophozoitesHemoglobinGene SilencingHorsesMolecular Biology TechniquesSequencing TechniquesEscherichia coliMolecular BiologyMicrobial PathogensBacterialcsh:REntamoebaOrganismsLectinBiology and Life SciencesProteinsbiology.organism_classificationParasitic Protozoansdigestive system diseasesProtein Subunits030104 developmental biologybiology.proteinProtozoaParasitologylcsh:QApicomplexaPurification TechniquesPLoS ONE
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Stopping injection attacks with code and structured data

2018

Injection attacks top the lists of the most harmful software vulnerabilities. Injection vulnerabilities are both commonplace and easy to exploit, which makes development of injection protection schemes important. In this article, we show how injection attacks can be practically eliminated through the use of structured data paired with cryptographic verification codes upon transmission. peerReviewed

0301 basic medicineExploitComputer scienceCross-site scriptingCryptographyComputer securitycomputer.software_genreSQL injection03 medical and health sciences0302 clinical medicineSoftwareSQL injectionCode (cryptography)Cryptographic hash functionProof-carrying codeproof-carrying codetietoturvaSQLbusiness.industryXSS030104 developmental biologyinjection030220 oncology & carcinogenesiscryptographic hashbusinesscomputer
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