Search results for "ORGANISMS"

showing 10 items of 726 documents

High and uneven levels of 45S rDNA site-number variation across wild populations of a diploid plant genus (Anacyclus, Asteraceae)

2017

The nuclear genome harbours hundreds to several thousand copies of ribosomal DNA. Despite their essential role in cellular ribogenesis few studies have addressed intrapopulation, interpopulation and interspecific levels of rDNA variability in wild plants. Some studies have assessed the extent of rDNA variation at the sequence and copy-number level with large sampling in several species. However, comparable studies on rDNA site number variation in plants, assessed with extensive hierarchical sampling at several levels (individuals, populations, species) are lacking. In exploring the possible causes for ribosomal loci dynamism, we have used the diploid genus Anacyclus (Asteraceae) as a suitab…

0106 biological sciences0301 basic medicineHereditylcsh:MedicineAsteraceae01 natural sciencesGenuslcsh:ScienceAnacyclusIn Situ Hybridization FluorescenceFlowering PlantsHeterozygosityMultidisciplinarybiologyChromosome BiologyEukaryotaPlantsKaryotypesPloidyResearch ArticleChromosome Structure and FunctionEvolutionary ProcessesContext (language use)DNA RibosomalChromosomes PlantChromosomesPolyploidyAnacyclusCytogenetics03 medical and health sciencesPolyploidBotanyGenetic variationGeneticsHybridizationRibosomal DNAEvolutionary Biologylcsh:ROrganismsGenetic VariationBiology and Life SciencesCell BiologyRibosomal RNAbiology.organism_classificationDiploidy030104 developmental biologyRNA RibosomalGenetic LociEvolutionary biologyKaryotypinglcsh:QDepartures from Diploidy010606 plant biology & botanyPLOS ONE
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Novel mutations in the voltage-gated sodium channel of pyrethroid-resistant Varroa destructor populations from the Southeastern USA

2016

The parasitic mite Varroa destructor has a significant worldwide impact on bee colony health. In the absence of control measures, parasitized colonies invariably collapse within 3 years. The synthetic pyrethroids tau-fluvalinate and flumethrin have proven very effective at managing this mite within apiaries, but intensive control programs based mainly on one active ingredient have led to many reports of pyrethroid resistance. In Europe, a modification of leucine to valine at position 925 (L925V) of the V. destructor voltage-gated sodium channel was correlated with resistance, the mutation being found at high frequency exclusively in hives with a recent history of pyrethroid treatment. Here,…

0106 biological sciences0301 basic medicineInsecticidesVeterinary medicineGene Identification and Analysislcsh:MedicineMicrobiologiaVoltage-Gated Sodium ChannelsFlumethrinmedicine.disease_causeBiochemistry01 natural sciencesAbellesInsecticide Resistancechemistry.chemical_compoundMethioninePyrethrinsÀcarsDestructorAmino Acidslcsh:ScienceMitesMutationMultidisciplinaryPyrethroidbiologyOrganic CompoundsGeneral MedicineBeesSoutheastern United StatesInsectsChemistryPhysical SciencesInsect ProteinsGeneral Agricultural and Biological SciencesResearch ArticleArthropodaApiaryParàsitsVarroidaeGeneral Biochemistry Genetics and Molecular Biology03 medical and health sciencesLeucineparasitic diseasesGeneticsMitemedicineAnimalsPoint MutationSulfur Containing Amino AcidsIsoleucineMutation DetectionAllelesbusiness.industryPoint mutationOrganic Chemistrylcsh:ROrganismsChemical CompoundsBiology and Life SciencesProteinsbiology.organism_classificationInvertebratesHymenopteraBiotechnology010602 entomology030104 developmental biologyAliphatic Amino AcidschemistryGenetic LociVarroa destructorMutationlcsh:Qbusiness
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The long journey of Orthotrichum shevockii (Orthotrichaceae, Bryopsida): From California to Macaronesia

2019

Biogeography, systematics and taxonomy are complementary scientific disciplines. To understand a species' origin, migration routes, distribution and evolutionary history, it is first necessary to establish its taxonomic boundaries. Here, we use an integrative approach that takes advantage of complementary disciplines to resolve an intriguing scientific question. Populations of an unknown moss found in the Canary Islands (Tenerife Island) resembled two different Californian endemic species: Orthotrichum shevockii and O. kellmanii. To determine whether this moss belongs to either of these species and, if so, to explain its presence on this distant oceanic island, we combined the evaluation of…

0106 biological sciences0301 basic medicineLeavesPlant ScienceBryology01 natural sciencesGeographical locationsCaliforniaNonvascular PlantsPhylogenyData ManagementMultidisciplinaryPhylogenetic analysisbiologyPlant AnatomyQREukaryotaPhylogenetic AnalysisPlantsClassification3. Good healthPhylogeneticsPhylogeographyGeographyMedicineTaxonomy (biology)Research ArticleSystematicsComputer and Information SciencesScienceBiogeographyCanary Islands010603 evolutionary biologyEvolution Molecular03 medical and health sciencesOrthotrichumMossesEvolutionary SystematicsNonvascular plantsOrthotrichaceaeEndemismTaxonomyEvolutionary BiologyBotánicaOrganismsBiology and Life SciencesSequence Analysis DNA15. Life on landbiology.organism_classificationBryopsidaUnited States030104 developmental biologyTaxonSpainEvolutionary biologyAfricaNorth AmericaBiological dispersalPeople and places
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Co-infection with iflaviruses influences the insecticidal properties of Spodoptera exigua multiple nucleopolyhedrovirus occlusion bodies: Implication…

2017

Biological insecticides based on Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) can efficiently control S. exigua larvae on field and greenhouse crops in many parts of the world. Spanish wild populations and laboratory colonies of S. exigua are infected by two iflaviruses (SeIV-1 and SeIV-2). Here we evaluated the effect of iflavirus co-infection on the insecticidal characteristics of SeMNPV occlusion bodies (OBs). Overall, iflavirus co-inoculation consistently reduced median lethal concentrations (LC50) for SeMNPV OBs compared to larvae infected with SeMNPV alone. However, the speed of kill of SeMNPV was similar in the presence or absence of the iflaviruses. A reduction of the we…

0106 biological sciences0301 basic medicineLife CyclesInsecticidesPhysiologyBiosecuritySeMNPV occlusion bodieslcsh:MedicineInsectPathogenesisPathology and Laboratory MedicineWeight Gain01 natural sciencesIflavirus co-infectionLarvaeInvertebrate GenomicsMedicine and Health Scienceslcsh:Sciencemedia_commonLarvaMultidisciplinaryCoinfectionAgricultureGenomicsInsectsPhysiological ParametersAgrochemicalsResearch ArticleArthropodamedia_common.quotation_subjectBiologySpodopteraSpodopteraMicrobiologyLepidoptera genitalia03 medical and health sciencesExtraction techniquesExiguaGeneticsAnimalsPest Control BiologicalBiological InsecticidesInoculationlcsh:RfungiBody WeightOrganismsBiology and Life SciencesPesticidebiology.organism_classificationInvertebratesRNA extractionNucleopolyhedrovirusesResearch and analysis methods010602 entomology030104 developmental biologyBiological insecticidesAnimal Genomicslcsh:QDevelopmental BiologyPLoS ONE
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Response to formal comment on Myhrvold (2016) submitted by Griebeler and Werner (2017)

2018

In his 2016 paper, Myhrvold criticized ours from 2014 on maximum growth rates (Gmax, maximum gain in body mass observed within a time unit throughout an individual’s ontogeny) and thermoregulation strategies (ectothermy, endothermy) of 17 dinosaurs. In our paper, we showed that Gmax values of similar-sized extant ectothermic and endothermic vertebrates overlap. This strongly questions a correct assignment of a thermoregulation strategy to a dinosaur only based on its Gmax and (adult) body mass (M). Contrary, Gmax separated similar-sized extant reptiles and birds (Sauropsida) and Gmax values of our studied dinosaurs were similar to those seen in extant similar-sized (if necessary scaled-up) …

0106 biological sciences0301 basic medicineMetabolic AnalysisPhysiologylcsh:MedicineAnimal Phylogenetics01 natural sciencesDinosaursBody TemperatureExtant taxonOrnithologyMaximum gainMedicine and Health SciencesGrowth rateSauropsidalcsh:ScienceArchosauriaData ManagementMammalsMultidisciplinarybiologyVertebrateEukaryotaPrehistoric AnimalsThermoregulationPhylogeneticsBioassays and Physiological AnalysisPhysiological ParametersEctothermVertebratesRegression AnalysisComputer and Information SciencesVertebrate PaleontologyZoologyResearch and Analysis Methods010603 evolutionary biologyFormal CommentBirds03 medical and health sciencesbiology.animalBasal Metabolic Rate MeasurementAnimalsAnimal PhysiologyEvolutionary SystematicsPaleozoologyTaxonomyEvolutionary Biologylcsh:ROrganismsBiology and Life SciencesPaleontologyReptilesbiology.organism_classificationBird Physiology030104 developmental biologyAmniotesEarth Scienceslcsh:QAllometryPaleobiologyZoologyPLoS ONE
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Dinosaur Metabolism and the Allometry of Maximum Growth Rate

2016

In his 2016 paper, Myhrvold criticized ours from 2014 on maximum growth rates (Gmax, maximum gain in body mass observed within a time unit throughout an individual’s ontogeny) and thermoregulation strategies (ectothermy, endothermy) of 17 dinosaurs. In our paper, we showed that Gmax values of similar-sized extant ectothermic and endothermic vertebrates overlap. This strongly questions a correct assignment of a thermoregulation strategy to a dinosaur only based on its Gmax and (adult) body mass (M). Contrary, Gmax separated similar-sized extant reptiles and birds (Sauropsida) and Gmax values of our studied dinosaurs were similar to those seen in extant similar-sized (if necessary scaled-up) …

0106 biological sciences0301 basic medicineMetabolic stateMetabolic AnalysisPhysiologylcsh:MedicineAnimal Phylogenetics01 natural sciencesBody TemperatureDinosaursMathematical and Statistical TechniquesExtant taxonMedicine and Health SciencesBody SizeGrowth ratelcsh:Sciencemedia_commonArchosauriaData ManagementMammalsMultidisciplinaryEcologyFossilsEukaryotaRegression analysisPrehistoric AnimalshumanitiesCurve FittingPhylogeneticsBioassays and Physiological AnalysisPhysiological ParametersEctothermPhysical SciencesVertebratesRegression AnalysisStatistics (Mathematics)Research ArticleComputer and Information Sciencesmedia_common.quotation_subjectVertebrate PaleontologyBiologyResearch and Analysis Methods010603 evolutionary biologyMarsupialsFormal CommentBirds03 medical and health sciencesBasal Metabolic Rate MeasurementAnimalsEvolutionary SystematicsStatistical MethodsPaleozoologyTaxonomyEvolutionary BiologyVariableslcsh:ROrganismsReptilesBiology and Life SciencesPaleontology030104 developmental biologyEvolutionary biologyBasal metabolic rateAmniotesEarth Scienceslcsh:QAllometryPaleobiologyEnergy MetabolismZoologyMathematical FunctionsMathematicsPLoS ONE
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Evolutionary relationships between digeneans of the family Brachycladiidae Odhner, 1905 and their marine mammal hosts: A cophylogenetic study.

2016

Cophylogenetic studies examine the congruence between host and parasite phylogenies. There are few studies that quantify the relative contribution of coevolutionary events, i.e. duplication, loss, failure-to-diverge, host-switching and spreading in trophically-transmitted parasites at the marine realm. We addressed this issue in the Brachycladiidae, a cosmopolitan digenean family specific to marine mammals. We used, for the first time, distance-based and event-based methods to explicitly test the coevolutionary events that have shaped the current brachycladiid-marine mammal associations. Parasite phylogeny was constructed using mtDNA ND3 sequences of nine brachycladiid species, and host phy…

0106 biological sciences0301 basic medicineMitochondrial DNAAquatic OrganismsBiology010603 evolutionary biology01 natural sciencesDNA MitochondrialDigeneaHost-Parasite Interactions03 medical and health sciencesSpecies SpecificityPhylogeneticsAnimalsPhylogenyMammalsCytochrome bHost (biology)EcologySequence Analysis DNACytochromes bbiology.organism_classificationBiological Evolution030104 developmental biologyInfectious DiseasesTaxonEvolutionary biologyDelphinoideaParasitologyMammalTrematodaParasitology international
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Molecular signatures of silencing suppression degeneracy from a complex RNA virus

2021

As genomic architectures become more complex, they begin to accumulate degenerate and redundant elements. However, analyses of the molecular mechanisms underlying these genetic architecture features remain scarce, especially in compact but sufficiently complex genomes. In the present study, we followed a proteomic approach together with a computational network analysis to reveal molecular signatures of protein function degeneracy from a plant virus (as virus-host protein-protein interactions). We employed affinity purification coupled to mass spectrometry to detect several host factors interacting with two proteins of Citrus tristeza virus (p20 and p25) that are known to function as RNA sil…

0106 biological sciences0301 basic medicineProteomicsCitrusInteraction NetworksPathogenesisPlant Sciencemedicine.disease_causePathology and Laboratory Medicine01 natural sciencesInteractomeBiochemistryBimolecular fluorescence complementationRNA interferenceRNA silencing supressorsCitrus tristeza virusMedicine and Health SciencesDegeneracy (biology)Protein Interaction MapsBiology (General)H20 Plant diseasesPlant ProteinsEcologybiologyPlant virusesEukaryotaArgonautePlantsSmall interfering RNANucleic acidsRNA silencingComputational Theory and MathematicsGenetic interferenceExperimental Organism SystemsModeling and SimulationProteomeArgonaute ProteinsHost-Pathogen InteractionsRNA ViralEpigeneticsResearch ArticleClosterovirusRNA virusViral proteinQH301-705.5Arabidopsis ThalianaPlant PathogensComputational biologyGenome ViralBrassicaResearch and Analysis MethodsModels BiologicalPlant Viral Pathogens03 medical and health sciencesCellular and Molecular NeuroscienceViral ProteinsModel OrganismsPlant and Algal ModelsTobaccomedicineGeneticsGenomesNon-coding RNAProtein InteractionsMolecular signaturesMolecular BiologyEcology Evolution Behavior and SystematicsPlant DiseasesHost Microbial InteractionsBiology and life sciencesMass spectrometryOrganismsComputational BiologyProteinsRNA virusPlant Pathologybiology.organism_classificationGene regulationRepressor Proteins030104 developmental biologyU30 Research methodsAnimal StudiesRNAGene expression010606 plant biology & botanyF30 Plant genetics and breeding
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Arbuscular mycorrhizal symbiosis mitigates the negative effects of salinity on durum wheat

2017

Arbuscular mycorrhizal (AM) symbiosis is generally considered to be effective in ameliorating the plant tolerance to salt stress. Unfortunately, the comprehension of the mechanisms implicated in salinity stress alleviation by AM symbiosis is far from being complete. Thus, an experiment was performed by growing durum wheat (Triticum durum Desf.) plants under salt-stress conditions to evaluate the influence of AM symbiosis on both the plant growth and the regulation of a number of genes related to salt stress and nutrient uptake. Durum wheat plants were grown outdoors in pots in absence or in presence of salt stress and with or without AM fungi inoculation. The inoculum consisted of a mixture…

0106 biological sciences0301 basic medicineRhizophagus irregularisSalinityLeavesGene Expressionlcsh:MedicinePlant SciencePlant RootsPolymerase Chain ReactionPhysical Chemistry01 natural sciencesNutrientMycorrhizaePlant Resistance to Abiotic Stresslcsh:ScienceTriticumBiomass (ecology)MultidisciplinaryEcologyPlant Anatomyfood and beveragesSalt TolerancePlantsSettore AGR/02 - Agronomia E Coltivazioni ErbaceeChemistryPlant PhysiologyPhysical SciencesWheatSymbiosiResearch ArticleBiology03 medical and health sciencesSymbiosisSettore AGR/07 - Genetica AgrariaPlant-Environment InteractionsBotanyGeneticsPlant DefensesGene RegulationGrassesSymbiosisBiochemistry Genetics and Molecular Biology (all)InoculationGene Expression ProfilingPlant EcologyEcology and Environmental Scienceslcsh:RfungiOrganismsFungiBiology and Life SciencesPlant RootPlant Pathologybiology.organism_classificationSporeSalinitySpecies Interactions030104 developmental biologyAgricultural and Biological Sciences (all)Chemical PropertiesArbuscular mycorrhizal symbiosislcsh:QSalt-Tolerance010606 plant biology & botanyPLOS ONE
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An exhaustive phylogeny of the combtooth blenny genus Salaria (Pisces, Blenniidae) shows introgressive hybridization and lack of reciprocal mtDNA mon…

2019

A comprehensive phylogeny of the genus Salaria based on mitochondrial and nuclear markers grouped the extant species of the genus in well-characterised marine and freshwater clades, thus rejecting the hypothesis of a polytypic origin of the freshwater Salaria populations and supporting the occurrence of a single invasion event of the inland waters by the genus. Based on both mitochondrial and nuclear DNA datasets, the Salaria species of the freshwater clade proved to be vicariant taxa originating from a common ancestor which could possibly spread throughout the circum-Mediterranean inland waters during the late Miocene Messinian salinity crisis, then experiencing a process of allopatric dif…

0106 biological sciences0301 basic medicineSalariaAquatic OrganismsMito-nuclear discordance Speciation without monophyly Introgressive hybridization Asymmetrical mtDNA introgressionCombtooth blennySettore BIO/05 - ZoologiaAllopatric speciation010603 evolutionary biology01 natural sciencesDNA barcodingDNA MitochondrialEvolution Molecular03 medical and health sciencesMonophylySpecies SpecificityGenusAsymmetrical mtDNA introgressionGeneticsAnimalsCladeMolecular BiologyEcology Evolution Behavior and SystematicsPhylogenyCell NucleusbiologyPhylogenetic treeGeographyBayes Theorembiology.organism_classificationIntrogressive hybridizationMitochondriaPerciformesSpeciation without monophyly030104 developmental biologyHaplotypesEvolutionary biologyHybridization GeneticMito-nuclear discordanceMolecular phylogenetics and evolution
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