Search results for "OTI"

Cytosolic calcium rises and related events in ergosterol-treated Nicotiana cells

2011

International audience; The typical fungal membrane component ergosterol was previously shown to trigger defence responses and protect plants against pathogens. Most of the elicitors mobilize the second messenger calcium, to trigger plant defences. We checked the involvement of calcium in response to ergosterol using Nicotiana plumbaginifolia and Nicotiana tabacum cv Xanthi cells expressing apoaequorin in the cytosol. First, it was verified if ergosterol was efficient in these cells inducing modifications of proton fluxes and increased expression of defence-related genes. Then, it was shown that ergosterol induced a rapid and transient biphasic increase of free [Ca2þ]cyt which intensity dep…

Genome reduction of the aphid endosymbiont Buchnera aphidicola in a recent evolutionary time scale.

2007

International audience; Genome reduction, a typical feature of symbiotic bacteria, was analyzed in the last stages of evolution of Buchnera aphidicola, the primary aphid endosymbiont, in two neutrally evolving regions: the pseudogene cmk and an intergenic region. These two regions were examined in endosymbionts from several lineages of their aphid host Rhopalosiphum padi, and different species of the same genus, whose divergence times ranged from 0.62 to 19.51 million years. Estimates of nucleotide substitution rates were between 4.3 and 6.7 x 10(-9) substitution/site/year, with G or C nucleotides being substituted around four times more frequently than A or T. Two different types of indel …

A molecular phylogeny for the leaf-roller moths (Lepidoptera: Tortricidae) and its implications for classification and life history evolution.

2012

BackgroundTortricidae, one of the largest families of microlepidopterans, comprise about 10,000 described species worldwide, including important pests, biological control agents and experimental models. Understanding of tortricid phylogeny, the basis for a predictive classification, is currently provisional. We present the first detailed molecular estimate of relationships across the tribes and subfamilies of Tortricidae, assess its concordance with previous morphological evidence, and re-examine postulated evolutionary trends in host plant use and biogeography.Methodology/principal findingsWe sequenced up to five nuclear genes (6,633 bp) in each of 52 tortricids spanning all three subfamil…

2020

Abstract Lichens are valuable models in symbiosis research and promising sources of biosynthetic genes for biotechnological applications. Most lichenized fungi grow slowly, resist aposymbiotic cultivation, and are poor candidates for experimentation. Obtaining contiguous, high-quality genomes for such symbiotic communities is technically challenging. Here, we present the first assembly of a lichen holo-genome from metagenomic whole-genome shotgun data comprising both PacBio long reads and Illumina short reads. The nuclear genomes of the two primary components of the lichen symbiosis—the fungus Umbilicaria pustulata (33 Mb) and the green alga Trebouxia sp. (53 Mb)—were assembled at contiguit…

Seasonal fluctuations in leaf phenolic composition under UV manipulations reflect contrasting strategies of alder and birch trees

2010

Seasonal variation in leaf phenolic composition may be important for acclimation of plants to seasonal changes in their biotic and abiotic environment. For a realistic assessment of how plants respond to solar UV-B (280-315 nm) and UV-A (315-400 nm) radiation, seasonal variation in both environment and plant responses needs to be taken into account. This also has implications for studies concerning stratospheric ozone depletion and resulting increased UV-B radiation, as other environmental variables and/or plant phenology could interact with UV radiation. To elucidate this, we established a field experiment using plastic films attenuating different parts of the solar UV spectrum. The concen…

Hierarchy of factors impacting grape berry mass: separation of direct and indirect effects on major berry metabolites

2018

Final berry mass, a major quality factor in wine production, is determined by the integrated effect of biotic and abiotic factors that can also influence berry composition. Under field conditions, interactions between these factors complicate study of the variability of berry mass and composition. Depending on the observation scale, the hierarchy of the impact degree of these factors can vary. The present work examines the simultaneous effects of the major factors influencing berry mass and composition to create a hierarchy by impact degree. A second objective was to separate the possible direct effects of factors on berry composition from an indirect effect mediated through their impact on…

Environmental change and disease dynamics: effects of intensive forest management on Puumala hantavirus infection in boreal bank vole populations.

2012

Intensive management of Fennoscandian forests has led to a mosaic of woodlands in different stages of maturity. The main rodent host of the zoonotic Puumala hantavirus (PUUV) is the bank vole (Myodes glareolus), a species that can be found in all woodlands and especially mature forests. We investigated the influence of forest age structure on PUUV infection dynamics in bank voles. Over four years, we trapped small mammals twice a year in a forest network of different succession stages in Northern Finland. Our study sites represented four forest age classes from young (4 to 30 years) to mature (over 100 years) forests. We show that PUUV-infected bank voles occurred commonly in all forest age…

Arabidopsis thaliana nicotianamine synthase 4 is required for proper response to iron deficiency and to cadmium exposure.

2013

International audience; The nicotianamine synthase (NAS) enzymes catalyze the formation of nicotianamine (NA), a non-proteinogenic amino acid involved in iron homeostasis. We undertook the functional characterization of AtNAS4, the fourth member of the Arabidopsis thaliana NAS gene family. A mutant carrying a T-DNA insertion in AtNAS4 (atnas4), as well as lines overexpressing AtNAS4 both in the atnas4 and the wild-type genetic backgrounds, were used to decipher the role of AtNAS4 in NA synthesis, iron homeostasis and the plant response to iron deficiency or cadmium supply. We showed that AtNAS4 is an important source for NA. Whereas atnas4 had normal growth in iron-sufficient medium, it dis…

Regulation of endocytosis by external stimuli in plant cells

2009

International audience; Endocytosis is an essential process for the normal growth and development of eukaryotic cells. This mini-review presents an overview of endocytosis regulation in challenging environmental conditions such as nutrient availability, hormonal treatment or abiotic/biotic stress in plant cells. Recent data establishing a relationship between endocytosis and reactive oxygen species (ROS) production are also discussed.

Raspberry breeding and biotisation for increasing plant stress tolerance and antioxidant activity

2008

International audience; Chemical inputs used in red fruit production can be detrimental to the environment and pesticides are not always efficient in controlling plant diseases. In addition, it is suspected that conventional agricultural practices have a negative impact on antioxidant activities in red fruits. Therefore new cultural approaches are required in order to produce red fruits of high quality. This involves an integrated programme of plant breeding and development of sustainable agricultural practices. Antioxidant activity was compared in leaves and berries of six raspberry cultivars for the selection of varieties for a breeding programme. This showed that cultivars that had the h…