Search results for "OTU"
showing 10 items of 2539 documents
Stepes strupastu galvas smadzeņu morfometriskā mainība guentheri grupā (Arvicolinae, Sumeriomys apakšģints)
2019
Šī darba mērķis ir galvas smadzeņu diverģences izpēte pēc to lieluma un ģeometriskās formas trim strupastu “guentheri” grupas taksoniem ar lineārās un ģeometriskās morfometrijas metožu izmantošanu. Iegūtie dati rāda, ka dzimuma faktors neietekmē izpētīto taksonu smadzeņu lineāros parametrus un formu, kas liecina par dzimuma atlases vāju iedarbību uz morfoloģisko pielāgojumu veidošanos un smadzeņu uzbūves mainību. Tiek atrastas būtiskas atšķirības strupastu galvas smadzeņu absolūtā un relatīvā masā (cefalizācijas indekss): vismazākā tā tiek atzīmēta M. guentheri, lielāka M. hartingi, bet savā starpā M. h. hartingi un M. h. lydius populācijas ievērojami diverģē. Visi lineārie mērījumi trim ta…
Acquisition of thermotolerance in sea urchin embryos correlates with the synthesis and age of the heat shock proteins.
1986
Preheating at 31 degrees C induces thermotolerance in Paracentrotus lividus embryos, which therefore become able to withstand 1-h treatment at the otherwise lethal temperature of 35 degrees C, and to develop normally. The acquisition of thermotolerance is positively correlated with the amount of heat shock proteins produced during the 31 degrees C treatment. Evidence is provided that the heat shock proteins, although present in the embryo for long periods after synthesis, lose their effect on thermotolerance within 3 h of the cessation of synthesis.
Amplification, contraction and genomic spread of a satellite DNA family (E180) in Medicago (Fabaceae) and allied genera
2011
†Background and Aims Satellite DNA is a genomic component present in virtually all eukaryotic organisms. The turnover of highly repetitive satellite DNA is an important element in genome organization and evolution in plants. Here we assess the presence and physical distribution of the repetitive DNA E180 family in Medicago and allied genera. Our goals were to gain insight into the karyotype evolution of Medicago using satellite DNA markers, and to evaluate the taxonomic and phylogenetic signal of a satellite DNA family in a genus hypothesized to have a complex evolutionary history. †Methods Seventy accessions from Medicago, Trigonella, Melilotus and Trifolium were analysed by PCR to assess …
Interaction mechanism of endogenous PP2A inhibitor protein ENSA with PP2A
2022
The vast diversity of protein phosphatase 2A (PP2A) holoenzyme composition ensures its multifaceted role in the regulation of cellular growth and signal transduction. In several pathological conditions, such as cancer, PP2A is inhibited by endogenous inhibitor proteins. Several PP2A inhibitor proteins have been identified, one of which is α-endosulfine (ENSA). ENSA inhibits PP2A activity when it is phosphorylated at Ser67 by Greatwall (Gwl) kinase. The role of ENSA in PP2A inhibition is rather well characterized, but knowledge of the mechanism of inhibition is scarce. In this study, we have performed comprehensive structural characterization of ENSA, and its interaction with PP2A A- and var…
The b1 isoform of protocadherin-gamma (Pcdhgamma) interacts with the microtubule-destabilizing protein SCG10.
2004
Due to their structural characteristics and their diversity, the 22 members of the protocadherin-gamma (Pcdhgamma) family have been suggested to contribute to the establishment of specific connections in the nervous system. Here, we focus on a single isoform, Pcdhgamma-b1. Its expression is found in different brain regions and in developing spinal cord it is restricted to scattered cells, whereas all cells are labeled using an antibody that recognizes all Pcdhgamma isoforms. As a first step to understanding the signaling mechanisms downstream of Pcdhgamma, we identify the microtubule-destabilizing protein SCG10 as a cytoplasmic interactor for Pcdhgamma-b1 and other isoforms of the Pcdhgamma…
SNAP-25a and -25b isoforms are both expressed in insulin-secreting cells and can function in insulin secretion
1999
The tSNARE (the target-membrane soluble NSF-attachment protein receptor, where NSF is N-ethylmaleimide-sensitive fusion protein) synaptosomal-associated protein of 25 kDa (SNAP-25) is expressed in pancreatic B-cells and its cleavage by botulinum neurotoxin E (BoNT/E) abolishes stimulated secretion of insulin. In the nervous system, two SNAP-25 isoforms (a and b) have been described that are produced by alternative splicing. Here it is shown, using reverse transcriptase PCR, that messages for both SNAP-25 isoforms are expressed in primary pancreatic B and non-B cells as well as in insulin-secreting cell lines. After transfection, both isoforms can be detected at the plasma membrane as well a…
Usher syndrome and Leber congenital amaurosis are molecularly linked via a novel isoform of the centrosomal ninein-like protein.
2009
Contains fulltext : 80984.pdf (Publisher’s version ) (Closed access) Usher syndrome (USH) and Leber congenital amaurosis (LCA) are autosomal recessive disorders resulting in syndromic and non-syndromic forms of blindness. In order to gain insight into the pathogenic mechanisms underlying retinal degeneration, we searched for interacting proteins of USH2A isoform B (USH2A(isoB)) and the LCA5-encoded protein lebercilin. We identified a novel isoform of the centrosomal ninein-like protein, hereby named Nlp isoform B (Nlp(isoB)), as a common interactor. Although we identified the capacity of this protein to bind calcium with one of its three EF-hand domains, the interacton with USH2A(isoB) did …
DNA sequence and pattern of expression of the sea urchin (Paracentrotus lividus) alpha-tubulin genes.
1989
To study the molecular aspects of the regulation of transcription of a multigene family, we have isolated and sequenced cDNA and genomic clones coding for the alpha-tubulin of the sea urchin Paracentrotus lividus. Two cDNA clones, P alpha 10 and P alpha 4, contain respectively the coding information for 391 C-terminal and for 338 N-terminal amino acids of the 452 residues that constitute the complete protein. They show silent nucleotide substitutions only, suggesting that P alpha 10 and P alpha 4 represent the cloned copies of two allelic gene transcripts, which encode for two alpha-tubulin isoforms with identical amino acid sequence in the region of the overlap. The comparison of the predi…
Synthesis of carbon nanotubes on FexOy doped Al2O3-ZrO2 nanopowder
2014
Carbon nanotubes (CNTs) were synthesized on liquid flame sprayed (LFS) powder substrate of iron oxide doped Al2O3-ZrO2. Iron oxide doped Al2O3-ZrO2 nanopowder was produced by injecting the liquid precursor of aluminium-isopropoxide, zirconium-n-propoxide, ferrocene and p-xylene into a high temperature (similar to 3000 K) flame. The precursor solution was atomized by high-velocity H-2 flow and injected into the flame where nanopartides were formed. The collected sample was used as a substrate material for the synthesis of CNTs. The CNTs were formed on the surfaces of the substrate powder by catalyzed decomposition of CH4. The particle morphology, size, phase composition and the nature of CNT…
Omission of Quality Software Development Practices : A Systematic Literature Review
2018
Software deficiencies are minimized by utilizing recommended software development and quality assurance practices. However, these recommended practices (i.e., quality practices) become ineffective if software professionals purposefully ignore them. Conducting a systematic literature review (n = 4,838), we discovered that only a small number of previous studies, within software engineering and information systems literature, have investigated the omission of quality practices. These studies explain the omission of quality practices mainly as a result of organizational decisions and trade-offs made under resource constraints or market pressure. However, our study indicates that different aspe…