Search results for "OXY"

showing 10 items of 11443 documents

Getting there and around: Host range oscillations during colonization of the Canary Islands by the parasitic nematode Spauligodon

2016

Episodes of expansion and isolation in geographic range over space and time, during which parasites have the opportunity to expand their host range, are linked to the development of host-parasite mosaic assemblages and parasite diversification. In this study, we investigated whether island colonization events lead to host range oscillations in a taxon of host-specific parasitic nematodes of the genus Spauligodon in the Canary Islands. We further investigated whether range oscillations also resulted in shifts in host breadth (i.e., specialization), as expected for parasites on islands. Parasite phylogeny and divergence time estimates were inferred from molecular data with Bayesian methods. H…

0106 biological sciences0301 basic medicineRange (biology)BiologyDNA Mitochondrial010603 evolutionary biology01 natural sciencesHost SpecificityHost-Parasite Interactions03 medical and health sciencesSpecies SpecificityOxyuroideaPhylogeneticsGeneticsAnimalsColonizationCladePhylogenyEcology Evolution Behavior and Systematicsgeographygeography.geographical_feature_categoryHost (biology)Bayes TheoremBiological EvolutionPhylogeography030104 developmental biologyTaxonSpainEvolutionary biologyArchipelagoMolecular Ecology
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Molecular phylogeny and forms of photosynthesis in tribe Salsoleae (Chenopodiaceae).

2016

Evolution of C3–C4 intermediate and C4 lineages are resolved in Salsoleae (Chenopodiaceae), and a model for structural and biochemical changes for the evolution of the Salsoloid form of C4 is considered.

0106 biological sciences0301 basic medicineRecurrent evolutionPhysiologyBlotting WesternPlant ScienceChenopodiaceaewestern blotsPhotosynthesis01 natural sciences03 medical and health sciencesMicroscopy Electron TransmissionBotanyPhotosynthesisChenopodiaceaeCladePhylogenyCarbon IsotopesbiologyPhylogenetic treeC2 pathway15. Life on landCarbon Dioxidebiology.organism_classificationGlycine Dehydrogenase (Decarboxylating)CO2 compensation pointPhenotypePlant Leaves030104 developmental biologyCompensation pointC3–C4 intermediatesMolecular phylogeneticsTEMleaf anatomyAncestral character state reconstruction010606 plant biology & botanyResearch PaperJournal of experimental botany
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Characterization and purification of a bacterial chlorogenic acid esterase detected during the extraction of chlorogenic acid from arbuscular mycorrh…

2016

International audience; A Gram-negative bacterium able to grow using chlorogenic acid (5-caffeoylquinic acid) as sole carbon source has been isolated from the roots of tomato plants inoculated with the arbuscular mycorrhizal fungus Rhizophagus irregularis. An intracellular esterase exhibiting very high affinity (K-m = 2 mu M) for chlorogenic acid has been extracted and purified by FPLC from the chlorogenate-grown cultures of this bacterium. The molecular mass of the purified esterase determined by SDS-PAGE was 61 kDa and its isoelectric point determined by chromatofocusing was 7.75. The esterase hydrolysed chlorogenic acid analogues (caffeoylshikimate, and the 4- and 3-caffeoylquinic acid i…

0106 biological sciences0301 basic medicineRhizophagus irregularisCoumaric AcidsPhysiologyRoot-associated bacteria[SDV]Life Sciences [q-bio]Arbuscular mycorrhizal fungiPlant ScienceBiologyCoumaric acidRoot exudates01 natural sciencesEsterasePlant RootsProtocatechuic acidSubstrate SpecificityFerulic acid03 medical and health scienceschemistry.chemical_compoundHydrolysisChlorogenic acidBacterial ProteinsSolanum lycopersicumMycorrhizaeGeneticsMethyl caffeate[SDV.BV]Life Sciences [q-bio]/Vegetal BiologyBacteriaEthanolMethanolChlorogenic acidbiology.organism_classification6. Clean waterChlorogenase030104 developmental biologychemistryBiochemistry[SDE]Environmental SciencesCarboxylic Ester Hydrolases010606 plant biology & botany
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C3cotyledons are followed by C4leaves: intra-individual transcriptome analysis ofSalsola soda(Chenopodiaceae)

2016

The genome of Salsola soda allows a transition from C3 to C4 photosynthesis. A developmental transcriptome series revealed novel genes showing expression patterns similar to those encoding C4 proteins.

0106 biological sciences0301 basic medicineSalsolaC4 photosynthesisfood.ingredientSalsolaPhysiologyPlant ScienceChenopodiaceaecotyledonBiology01 natural sciences03 medical and health sciencesfoodRNA seqBotanyPhotosynthesisChenopodiaceaedevelopmentSalsola sodaCarbon IsotopesleafCaryophyllalesGene Expression Profilingfood and beveragesbiology.organism_classificationCaryophyllalesPlant Leaves030104 developmental biologyMRNA SequencingSeedlingTranscriptomePhosphoenolpyruvate carboxylaseCotyledonResearch Paper010606 plant biology & botanyJournal of Experimental Botany
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Oxygen Availability during Growth Modulates the Phytochemical Profile and the Chemo-Protective Properties of Spinach Juice.

2018

Fruits and vegetables are a good source of potentially biologically active compounds. Their regular consumption in the human diet can help reduce the risk of developing chronic diseases such as cardiovascular diseases and cancer. Plants produce additional chemical substances when subject to abiotic stress or infected by microorganisms. The phytochemical profile of spinach leaves (Spinacia oleracea L.), which is a vegetable with widely recognized health-promoting activity, has been affected by applying root hypoxic and re-oxygenation stress during plant growth. Leaf juice at different sampling times has been subject to liquid chromatography mass spectrometry (LC-MSn) analysis and tested on t…

0106 biological sciences0301 basic medicineSpinaciaAntioxidantHT29 cell lineCell Survivalmedicine.medical_treatmentLiquid Chromatography-Mass Spectrometry<i>Spinacia oleracea</i> L.lcsh:QR1-502antioxidant activitySpinacia oleracea L.Anti-proliferative activity; Antioxidant activity; Comet Assay; HT29 cell line; Liquid Chromatography-Mass Spectrometry; Spinacia oleracea L;medicine.disease_cause01 natural sciencesBiochemistrylcsh:MicrobiologyAntioxidantsMass SpectrometryArticle03 medical and health sciencesSpinacia oleraceamedicineHumansFood scienceMolecular BiologyCell ProliferationbiologyAbiotic stressChemistryChemistry PhysicalPlant Extractsfood and beveragesBiological activitybiology.organism_classificationAntineoplastic Agents PhytogenicComet assayFruit and Vegetable JuicesOxygen030104 developmental biologyPhytochemicalSpinachanti-proliferative activityComet AssayDrug Screening Assays AntitumorHT29 CellsOxidative stress010606 plant biology & botanyChromatography LiquidBiomolecules
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Thioredoxin (Trxo1) interacts with proliferating cell nuclear antigen (PCNA) and its overexpression affects the growth of tobacco cell culture.

2017

Thioredoxins (Trxs), key components of cellular redox regulation, act by controlling the redox status of many target proteins, and have been shown to play an essential role in cell survival and growth. The presence of a Trx system in the nucleus has received little attention in plants, and the nuclear targets of plant Trxs have not been conclusively identified. Thus, very little is known about the function of Trxs in this cellular compartment. Previously, we studied the intracellular localization of PsTrxo1 and confirmed its presence in mitochondria and, interestingly, in the nucleus under standard growth conditions. In investigating the nuclear function of PsTrxo1 we identified proliferati…

0106 biological sciences0301 basic medicineTFs transcription factorsOverexpressionBiologíaBiFC bimolecular fluorescence complementationClinical BiochemistryCell Culture TechniquesTobacco BY-2 cells01 natural sciencesBiochemistryTBY-2 tobacco bright yellow-2DTT 14-dithiothreitolBimolecular fluorescence complementationThioredoxinsGene Expression Regulation PlantTrx thioredoxinlcsh:QH301-705.5GFP green fluorescent proteinlcsh:R5-920biologyProliferating cell nuclear antigen (PCNA)Cell cycleGlutathione3. Good healthCell biologyMitochondriaNTR NADPH thioredoxin reductaseProtein TransportDEM diethyl maleateRT-qPCR Reverse transcription quantitative polymerase chain reactionThioredoxinlcsh:Medicine (General)Oxidation-ReductionAMS 4-acetamido-4-maleimidylstilbene-22-disulfonic acidResearch PaperPCNA proliferating cell nuclear antigenOex overexpressingCell cycleNucleusThioredoxin o103 medical and health sciencesROS reactive oxygen speciesDownregulation and upregulationProliferating Cell Nuclear AntigenTobaccoDAPI 46-diamidine-2-phenylindolmCBM monochlorobimaneCellular compartmentCell NucleusCell growthOrganic ChemistryBotánicaPeasMolecular biologyYFP yellow fluorescent proteinProliferating cell nuclear antigenTBS Tris-buffered salineOD optical density030104 developmental biologylcsh:Biology (General)Cell cultureRNA reactive nitrogen speciesbiology.proteinPrx peroxiredoxinBSA bovine serum albumin010606 plant biology & botanyRedox biology
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Trichoderma harzianum Strain T22 Modulates Direct Defense of Tomato Plants in Response to Nezara viridula Feeding Activity

2021

AbstractPlant growth-promoting fungi belonging to genus Trichoderma are known to help plants when dealing with biotic stressors by enhancing plant defenses. While beneficial effects of Trichoderma spp. against plant pathogens have long been documented, fewer studies have investigated their effect on insect pests. Here, we studied the impact of Trichoderma root colonization on the plant defense responses against stink bug feeding attack. For this purpose, a model system consisting of tomato plant, Solanum lycopersicum cv Dwarf San Marzano, Trichoderma harzianum strain T22 and the southern green stink bug, Nezara viridula, was used. We firstly determined stink bug performance in terms of rela…

0106 biological sciences0301 basic medicineTime FactorsTranscription GeneticGreen stink bugBeneficial soil microbes Jasmonic acid signaling pathway Pentatomidae Solanum lycopersicum Stink bugsCyclopentanesGenes PlantPlant Roots01 natural sciencesBiochemistryArticleHeteroptera03 medical and health scienceschemistry.chemical_compoundSolanum lycopersicumGene Expression Regulation PlantPentatomidaePlant defense against herbivoryAnimalsHerbivoryOxylipinsSymbiosisStink bugsEcology Evolution Behavior and SystematicsbiologyBeneficial soil microbesJasmonic acidfungifood and beveragesTrichoderma harzianumGeneral MedicinePentatomidaebiology.organism_classificationHorticulture030104 developmental biologychemistryNezara viridulaJasmonic acid signaling pathwayTrichodermaHypocrealesSeedsFemaleSolanumSignal Transduction010606 plant biology & botany
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Polyamine Oxidase 5 loss-of-function mutations in Arabidopsis thaliana trigger metabolic and transcriptional reprogramming and promote salt stress to…

2017

The family of polyamine oxidases (PAO) in Arabidopsis (AtPAO1-5) mediates polyamine (PA) back-conversion, which reverses the PA biosynthetic pathway from spermine, and its structural isomer thermospermine (tSpm), into spermidine and then putrescine. Here, we have studied the involvement of PA back-conversion in Arabidopsis salinity tolerance. AtPAO5 is the Arabidopsis PAO gene member most transcriptionally induced by salt stress. Two independent loss-of-function mutants (atpao5-2 and atpao5-3) were found to exhibit constitutively higher tSpm levels, with associated increased salt tolerance. Using global transcriptional and metabolomic analyses, the underlying mechanisms were studied. Stimul…

0106 biological sciences0301 basic medicineTranscription GeneticArabidopsis thalianaPhysiologyArabidopsisSperminePlant ScienceSodium Chloride01 natural scienceschemistry.chemical_compoundGene Expression Regulation PlantLoss of Function MutationArabidopsisPolyaminesMetabolitesArabidopsis thalianaPoliaminesAbscisic acidPrincipal Component AnalysisbiologyAgricultural SciencesSalt ToleranceMetabòlitsmetabolomicsPhenotypeBiochemistryMultigene FamilyMetabolomeCitric Acid CycleSalsCyclopentanes03 medical and health sciencesStress PhysiologicalOxylipinsRNA MessengerIonssalt toleranceArabidopsis ProteinsGene Expression ProfilingSodiumHydrogen PeroxideAgriculture Forestry and Fisheriesbiology.organism_classificationSpermidineGene Ontology030104 developmental biologychemistrythermosperminePutrescineSpermineSaltsOxidoreductases Acting on CH-NH2 Group DonorsTranscriptomejasmonatesPolyaminePolyamine oxidaseAbscisic Acid010606 plant biology & botany
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Induction of radiata pine somatic embryogenesis at high temperatures provokes a long-term decrease in dna methylation/hydroxymethylation and differen…

2020

Based on the hypothesis that embryo development is a crucial stage for the formation of stable epigenetic marks that could modulate the behaviour of the resulting plants, in this study, radiata pine somatic embryogenesis was induced at high temperatures (23 &deg

0106 biological sciences0301 basic medicineanimal structuresSomatic embryogenesisSomatic cellheat shock proteinPlant Scienceepigenetics; 5-hydroxymethylcytosine; 5-methylcytosine; heat; heat shock protein; memory; Pinus radiata; priming; somatic embryo; somatic plantBiology01 natural sciencesArticleTranscriptomememory03 medical and health sciencessomatic embryoMemorylcsh:BotanyHeat shock proteinEpigenetics5-hydroxymethylcytosine5-methylcytosineprimingEcology Evolution Behavior and SystematicsPinus radiataHeat shock proteinEcologyepigeneticsEmbryogenesisfungiSomatic embryofood and beveragesMethylationHeat<i>Pinus radiata</i>lcsh:QK1-989Cell biologySomatic plant030104 developmental biologysomatic plantPrimingDNA methylationEpigeneticsheat010606 plant biology & botany
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Polyamine Biosynthesis Engineering as a Tool to Improve Plant Resistance to Abiotic Stress

2015

Polyamines (PAs) are small polycationic molecules which are present in all living organisms. PAs have been involved in a wide array of metabolic plant processes, extending from development to stress protection. Most of this knowledge has been achieved through the observation of PA homeostasis and manipulation of plant PA levels mediated by different approaches. This chapter summarizes the approaches undertaken to demonstrate the relationship between PAs and the stress response and, in particular, how the genetic manipulation of polyamine levels has evolved in a useful tool for the enhancement of plant stress tolerance in many species, including crops. This chapter also includes the most rec…

0106 biological sciences0301 basic medicinechemistry.chemical_classificationReactive oxygen speciesAbiotic stressfood and beveragesSpermineGenetically modified crops01 natural sciencesCell biologySpermidine03 medical and health scienceschemistry.chemical_compound030104 developmental biologychemistryBotanyPutrescinePolyamineAbscisic acid010606 plant biology & botany
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