Search results for "Orotidine"

showing 2 items of 2 documents

Differential Expression of the Aspergillus fumigatus pksP Gene Detected In Vitro and In Vivo with Green Fluorescent Protein

2001

ABSTRACT Aspergillus fumigatus is an important pathogen of immunocompromised hosts, causing pneumonia and invasive disseminated disease with high mortality. To be able to analyze the expression of putative virulence-associated genes of A. fumigatus , the use of the enhanced green fluorescent protein (EGFP) as a reporter was established. Two 5′ sequences, containing the putative promoters of the pyrG gene, encoding orotidine-5′-phosphate decarboxylase, and the pksP gene, encoding a polyketide synthase involved in both pigment biosynthesis and virulence of A. fumigatus , were fused with the egfp gene. The P pksP - egfp construct was integrated via homologous recombination into the genomic pks…

HyphaGenes FungalGreen Fluorescent ProteinsMolecular Sequence DataOrotidine-5'-Phosphate DecarboxylaseImmunologyFluorescence spectrometryGene ExpressionBiologyMicrobiologyMicrobiologyGreen fluorescent proteinAspergillus fumigatusConidiumGenes ReporterMultienzyme ComplexesGene expressionAmino Acid SequenceDNA FungalPathogenGeneBase SequenceAspergillus fumigatusfungibiology.organism_classificationArtificial Gene FusionLuminescent ProteinsInfectious DiseasesParasitologyFungal and Parasitic InfectionsInfection and Immunity
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Elucidating the catalytic reaction mechanism of orotate phosphoribosyltransferase by means of X-ray crystallography and computational simulations

2020

15 p.-8 fig.-2 tab.1 graph. abst.+ 8 fig. supl.-1 tab. supl.

PyrimidineStereochemistry010402 general chemistry01 natural sciencesCatalysisEnzyme catalysischemistry.chemical_compoundOrotidineNucleotideReaction mechanismchemistry.chemical_classificationMD simulations010405 organic chemistryHydrogen bondEnzyme catalysisGeneral ChemistryTautomer0104 chemical sciencesPyrimidine metabolismOrotate phosphoribosyltransferasechemistryPyrimidine metabolismOrotate phosphoribosyltransferaseQM/MM methodsX-ray structure
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