Search results for "Osmoprotectant"
showing 6 items of 6 documents
Effects of foliar application of glycine betaine and chitosan on Puccinellia distans (Jacq.) Pari, subjected to salt stress.
2019
Introduction:Using brackish water for irrigation may expose turfgrasses to salinity stress.Employing the best treatments to maintain high-quality turfs under saline conditions is animportant requirement for turfgrass management.Methods:We tested the response of ahalophyte grass,Puccinellia distans, to irrigation with saline solutions and to foliarapplication of two osmoprotectants, such as glycine betaine (GB) or chitosan (CH). Plantswere grown in pots under controlled conditions and irrigated with 200 mM or 600 mM ofNaCl solutions. The response to salinity treatments and osmoprotectant application wasevaluated after 90 days by measuring leaffiring, leaf density, shoot length and biomass, r…
Differences in metabolic profiles of planktonic and biofilm cells in Staphylococcus aureus - (1)H Nuclear Magnetic Resonance search for candidate bio…
2013
Staphylococcus aureus is responsible for many types of infections related to biofilm presence. As the early diagnostics remains the best option for prevention of biofilm infections, the aim of the work presented was to search for differences in metabolite patterns of S. aureus ATCC6538 biofilm vs. free-swimming S. aureus planktonic forms. For this purpose, Nuclear Magnetic Resonance (NMR) spectroscopy was applied. Data obtained were supported by means of Scanning Electron Microscopy, quantitative cultures and X-ray computed microtomography. Metabolic trends accompanying S. aureus biofilm formation were found using Principal Component Analysis (PCA). Levels of isoleucine, alanine and 2,3-but…
Genetic Engineering Strategies for Abiotic Stress Tolerance in Plants
2015
Crop plants are affected by a variety of abiotic stresses such as salinity, drought, extreme temperatures, and oxidative stress and cause a significant yield loss (more than 50 %). In the near future, these abiotic stresses might increase because of global climate change. Abiotic stresses lead to dehydration or osmotic stress through reduced availability of water for vital cellular functions and maintenance of turgor pressure and also result in high production of reactive oxygen species (ROS). Plants are evolved with various mechanisms such as changes in cellular and metabolic processes to cope with the stress condition. Recent developments in molecular genetics have contributed greatly to …
Bacteria-Derived Compatible Solutes Ectoine and 5α-Hydroxyectoine Act as Intestinal Barrier Stabilizers to Ameliorate Experimental Inflammatory Bowel…
2015
Earlier studies showed that the compatible solute ectoine (1) given prophylactically before induction of colitis by 2,4,6-trinitrobenzenesulfonic acid (TNBS) in rats prevented histological changes induced in the colon and the associated rise in inflammatory mediators. This study was therefore conducted to investigate whether ectoine (1) and its 5α-hydroxy derivative (2) would also be effective in treating an already established condition. Two days after inducing colitis in rats by instilling TNBS/alcohol in the colon, animals were treated orally once daily for 1 week with either 1 or 2 (50, 100, 300 mg/kg). Twenty-four hours after the last drug administration rats were sacrificed. Ulcerativ…
Impact of compatible solutes on the mechanical properties of fibronectin: a single molecule analysis
2003
The influence of ectoine and sarcosine on the mechanical properties of surface bound fibronectin has been investigated by means of force microscopy. Single molecule stretching experiments of fibronectin molecules reveal that ectoine and sarcosine increase the tendency of the polypeptide to coil, thus decreasing its apparent persistence length. This behavior can be explained by means of the preferential exclusion model implying that the osmolytes are expelled from the protein surface due to the increase in chemical potential of the denatured, i.e. stretched, state forcing the protein into a more compact structure. Detailed analysis of the unfolding forces, which are extracted from the succes…
Effectiveness of glycinebetaine foliar application in relieving salt stress symptoms in two turf-grasses
2014
Salinity is one of the problems that has to be dealt with in turf-grass management, due both to restrictions in freshwater use for landscape irrigation and exposition to salt stress in coastal areas. This has led to the need to select salinity tolerant genotypes and test management practices, such as the application of osmoprotectants, in order to maintain turf quality at acceptable costs. We compared the effects of irrigation with 600 mM NaCl and glycinebetaine (GB) application for 8 weeks on pot-grown weeping alkaligrass (Puccinellia distans (L.) Parl.) and tall fescue (Festuca arundinacea Schreb.). We selected two easily assessable parameters, total clippings fresh weight and chlorophyll…