Search results for "PAPER"

Synthesis and Evaluation of Novel Ring‐Strained Noncanonical Amino Acids for Residue‐Specific Bioorthogonal Reactions in Living Cells

2021

Abstract Bioorthogonal reactions are ideally suited to selectively modify proteins in complex environments, even in vivo. Kinetics and product stability of these reactions are crucial parameters to evaluate their usefulness for specific applications. Strain promoted inverse electron demand Diels–Alder cycloadditions (SPIEDAC) between tetrazines and strained alkenes or alkynes are particularly popular, as they allow ultrafast labeling inside cells. In combination with genetic code expansion (GCE)‐a method that allows to incorporate noncanonical amino acids (ncAAs) site‐specifically into proteins in vivo. These reactions enable residue‐specific fluorophore attachment to proteins in living mam…

Ceria/polymer nanocontainers for high-performance encapsulation of fluorophores

2019

We report the synthesis of high-performance organic–inorganic hybrid fluorescent nanocapsules comprising a polymer shell armored with an inorganic layer and a liquid core containing a fluorophore. The polymeric capsules are synthesized by free radical miniemulsion polymerization and contain covalently bound carboxylate surface functionalities that allow for the binding of metal ions through electrostatic interaction. A cerium(IV) oxide nanoparticle layer, formed in situ at the surface of the hybrid nanocapsules, acts as oxygen scavenger and keeps external reactive molecular oxygen from entering into the capsules, eventually resulting in a reduction of the photooxidation of encapsulated fluo…

Pulsed electric field applications for the extraction of compounds and fractions (fruit juices, winery, oils, by-products, etc.)

2020

Abstract The pulsed electric field (PEF) is well known to have a significant effect on the rate of various processes in the food industry. Food processes can be completed rapidly with higher efficiency, high reproducibility, reducing the processing cost, simplifying manipulation, and work-up, giving higher purity of the final product, eliminating posttreatment of wastewater and less energy when compared to a conventional process using the PEF. The advantages of using the PEF for food processing is to get high quality fresh-like liquid foods with excellent flavor, nutritional value, and shelf life retaining the fresh aroma, taste, and appearance. This chapter presents a complete picture of c…

Biomass Fractionation Using Emerging Technologies

2021

Small-sized granules of biphasic bone substitutes support fast implant bed vascularization

2015

The present study investigated the influence of granule size of 2 biphasic bone substitutes (BoneCeramic® 400-700 μm and 500-1000 μm) on the induction of multinucleated giant cells (MNGCs) and implant bed vascularization in a subcutaneous implantation model in rats. Furthermore, degradation mechanisms and particle phagocytosis of both materials were examined by transmission electron microscopy (TEM). Both granule types induced tissue reactions involving primarily mononuclear cells and only small numbers of MNGCs. Higher numbers of MNGCs were detected in the group with small granules starting on day 30, while higher vascularization was observed only at day 10 in this group. TEM analysis reve…

Discovering temporal relationships in databases of newspapers

1998

This paper is mainly dedicated to analyse the problem of discovering frequent temporal patterns in event sequences extracted from a large repository of newspapers. The proposed formalism and algorithms rely on Toodor, which is a document retrieval system that allows users to specify conditions over the structure, contents and temporal features of the stored documents. We develop in this work several algorithms for recognising frequent temporal patterns in terms of arc-consistency, which consist of discarding temporal occurrences that do not satisfy a temporal structure.

Estimation of formamide harmonic and anharmonic modes in the Kohn-Sham limit using the polarization consistent basis sets.

2010

Formamide harmonic and anharmonic frequencies of fundamental vibrations in the gas phase and in several solvents were successfully estimated in the B3LYP Kohn-Sham complete basis set limit (KS CBS). CBS results were obtained by extrapolating a power function (two-parameter formula) to the results calculated with polarization-consistent basis sets. Anharmonic corrections using the second order perturbation treatment (PT2) and hybrid B3LYP functional combined with polarization consistent pc-n (n = 0, 1, 2, 3, 4) and several Pople’s basis sets were analyzed for all fundamental formamide vibrational modes in the gas phase and solution. Solvent effects were modeled within a PCM method. The anhar…

Chromatographically separable rotamers of an unhindered amide

2014

Surprisingly stable formamide rotamers were encountered in the tetrahydroisoquinoline and morphinan series of alkaloids. We investigated the hindered rotation around the amide bond by dynamic high-performance liquid chromatography (DHPLC) and kinetic measurements of the interconversion of the rotamers which can readily be separated by HPLC as well as TLC. The experimental results of the different methods were compared to each other as well as to results obtained by DFT calculations.

Cerdioxid schützt vor marinem Fouling

2017

Victims or Aggresors? Framing Romanian Migrants and Crime in Two British Newspapers

2018

The research question at which the present article attempts to answer is: What are the British journalistic procedures used in representing migration and crime in the specific case of Romanian migrants? The article uses quantitative content analysis as the main method of research. The sample of texts was made up of 117 articles published in two (tabloid) British newspapers (the Daily Mail and the Daily Mirror) in the time-period January 1–December 21, 2015. The violent acts of Romanian migrants that were covered by the UK press were extremely diverse. They ranged from robbery, murder and begging to prostitution and medical malpractice. At the same time, Romanian migrants were portrayed not …