Search results for "PEST"

Development and Characterization of Diamondback Moth Resistance to Transgenic Broccoli Expressing High Levels of Cry1C

2000

ABSTRACT A field-collected colony of the diamondback moth, Plutella xylostella , had 31-fold resistance to Cry1C protoxin of Bacillus thuringiensis . After 24 generations of selection with Cry1C protoxin and transgenic broccoli expressing a Cry1C protein, the resistance that developed was high enough that neonates of the resistant strain could complete their entire life cycle on transgenic broccoli expressing high levels of Cry1C. After 26 generations of selection, the resistance ratios of this strain to Cry1C protoxin were 12,400- and 63,100-fold, respectively, for the neonates and second instars by a leaf dip assay. The resistance remained stable until generation 38 (G38) under continuous…

“Budapest”, “Berlino”, “Tallinn”, “Intervenire negli spazi di abitazione privati per rigenerare la città pubblica”

2004

Metodes izstrāde un validācija pesticīdu atliekvielu noteikšanai Camellia sinensis lapās ar AEŠH-MS/MS un GH-MS/MS

2021

Metodes izstrāde un validācija pesticīdu atliekvielu noteikšanai Camellia sinensis lapās ar AEŠH-MS/MS un GH-MS/MS. Lankovska A., zinātniskās vadītājas Dr.chem. Pugajeva I., M.Sc., LU doktorante Ikkere L.E. Maģistra darbs, 40 lappuses, 17 attēli, 8 tabulas, 34 literatūras avoti, 6 pielikumi. Latviešu valodā. Darbā apkopota literatūra par tēju ķīmisko sastāvu, pesticīdu klasifikāciju, oficiālajām paraugu sagatavošanas metodēm, traucējošajiem efektiem pesticīdu noteikšanā un Eiropas Komisijas izdotajiem validācijas standartiem. Darbā veikta metodes validācija 121 savienojuma noteikšanai kaltētās Camellia Sinensis lapās ar augsti efektīvo šķidrumu hromatogrāfiju apvienojumā ar tandēma masspekt…

Bacteria Associated with Copestylum (Diptera, Syrphidae) Larvae and Their Cactus Host Isolatocereus dumortieri

2011

We describe the gut bacterial diversity inhabiting two saprophagous syrphids and their breeding substrate (decayed tissues of the columnar cactus Isolatocereus dumortieri). We analyzed the gut microbiota of Copestylum latum (scooping larvae that feed on decayed cactus tissues) and Copestylum limbipenne (whose larvae can also feed on semiliquid tissues) using molecular techniques. DNA was extracted from larval guts and cactus tissues. The V1-V3 region of the 16S rRNA genes was amplified and sequenced. A total of 31079 sequences were obtained. The main findings are: C. limbipenne is dominated by several Enterobacteriaceae, including putative nitrogen-fixing genera and pectinolitic species and…

Architetture rupestri a Caltabellotta

2020

Questo saggio descrivere i caratteri tipologici, le evoluzioni nel tempo e le connotazioni costruttive dell'architettura rupestre a Caltabellotta (AG). L’Italia rupestre, nella sua totalità, può – e non a torto - essere considerata un plusvalore se non ancora del tutto da scoprire, sicuramente da valorizzare e re-interpretare nel progetto di architettura e fruizione dei beni culturali e paesaggistici; non a caso, sovente le aree rupestri rappresentano un sistema attrattivo oggetto di tutela, inserito in piani paesistici e protetto da un vincolo di riserva naturale in ambito regionale, o ancora inserito in istituiti o istituendi distretti turistici. Il saggio ha analizzato cavità artificiali…

Plant sterol oxides in functional beverages: Influence of matrix and storage

2014

Three plant sterol (PS)-enriched beverages, milk based fruit juice (MFJPS), fruit juice (FJPS) and milk beverage (MPS), were stored at 4, 24, or 37 °C and analysed at regular time intervals of 2 months until 6 months. PS stability was analysed from the production of phytosterol oxidation products (POPs). The β-sitosterol oxides (7α/7β-hydroxy, β/α-epoxy, triol, and 7-keto) and campesterol oxides (β/α-epoxy, and 7-keto) were detected in all beverages and at all storage times and temperatures. Total POP contents followed the order MPS≫FJPSMFJPS. In general, the beverages showed low PS oxidation levels (0.17%). Predictive models of POP content versus storage time were established. These models…

Recent Advances in Sample Preparation for Pesticide Analysis

2012

Much progress has been made in pesticide analysis in the past decade. During this time, hyphenated techniques involving highly efficient separation with sensitive mass spectrometric detection have become the methods of choice. Even with such powerful instrumental techniques, a demanding task in pesticide residue analysis is the development of multiresidue methods for the determination of pesticides in air, water, soil, sediment, biota, and food. This chapter summarizes the analytical characteristics of the different methods of sample preparation for the determination of pesticide residues in a variety of food matrices, and surveys their recent applications in combination with gas chromatogr…

Odorants of Capsicum spp. Dried Fruits as Candidate Attractants for Lasioderma serricorne F. (Coleoptera: Anobiidae)

2021

The cigarette beetle, Lasioderma serricorne F. (Coleoptera: Anobiidae) is an important food storage pest affecting the tobacco industry and is increasingly impacting museums and herbaria. Monitoring methods make use of pheromone traps which can be implemented using chili fruit powder. The objective of this study was to assess the response of L. serricorne to the volatile organic compounds (VOCs) from different chili powders in order to identify the main semiochemicals involved in this attraction. Volatiles emitted by Capsicum annuum, C. frutescens, and C. chinense dried fruit powders were tested in an olfactometer and collected and analyzed using SPME and GC-MS. Results indicated that C. an…

EFFECTS OF THIOBENCARB HERBICIDE TO AN ALGA (NANNOCHLORIS OCULATA) AND THE CLADOCERAN (DAPHNIA MAGNA)

2001

Chronic toxicity studies were conducted with an algae (Nannochloris oculata) and the cladoceran (Daphnia magna) to determine their relative sensitivities to the thiocarbamate herbicide thiobencarb (S-4-chlorobenzyl diethylthiocarbamate). Most of the algal populations were initially affected by exposure to the herbicide. Thiobencarb concentrations higher than 0.5 mg/L significantly reduced algal densities after 24-h exposure. The 24-h static EC50 in D. magna was 3.01 mg/L. The sublethal effects of 0.3, 0.37, 0.5, 0.75, and 1.5 mg/L of thiobencarb concentrations on the survival, reproduction, and growth of D. magna were monitored for 21 days. The parameters used to determined the effect of th…

Chronic cumulative risk assessment of the exposure to organophosphorus, carbamate and pyrethroid and pyrethrin pesticides through fruit and vegetable…

2015

In the present study, the chronic cumulative exposure to organophosphorus (OPs), carbamates (CBs) and pyrethroid and pyrethrin (PPs) pesticides in the region of Valencia through fruit and vegetables consumption is presented. A total of 752 samples and 84 pesticides were studied of which, 52 were OPs, 23 CBs and 9 PPs. Residue data were derived from the Valencia Region monitoring program 2007-2011 and food consumption levels from a questionnaire-based dietary survey conducted in 2010 in the same area. The relative potency factor (RPFs) approach was used to estimate chronic cumulative dietary exposure to OPs, CBs and PPs using acephate, oxamyl and deltamethrin as index compounds, respectively…