Search results for "PET"

Direct detection of repetitive, whole chromosome paint and telomere DNA probes by immunogold electron microscopy

1993

Biotinylated repetitive, whole chromosome paint and telomere DNA probes were investigated at the electron microscope level after non-isotopic in situ hybridization and direct immunogold detection. The protocol described allowed the visualization of a biotinylated chromosome 1 specific satellite DNA probe in the light microscope without silver intensification. This sensitive method was exploited to analyse factors contributing to signal strength in immunogold chromosome painting. Furthermore, it allowed us to investigate the distribution of (TTAGGG)n telomere repeats in human metaphase chromosomes and interphase nuclei. Telomeric and internal (TTAGGG)n repeats were detected at high spatial r…

A low repeat length in oligodendrocyte chromatin

1985

Abstract: The behavior of oligodendrocyte chromatin after micrococcal nuclease digestion of nuclei was assayed in brains of rats of four different ages. During oligodendrocyte differentiation, a decreasing sensitivity of the chromatin to enzymatic attack was observed. On the other hand, the nucleosomal repeat length showed a slight tendency to increase during development. It is worth noting that even the highest values reported here for “oligodendrocyte’ chromatin repeat lengths are significantly lower than 200 base pairs, the value previously reported by others for “non‐astrocytic glia.” Copyright © 1985, Wiley Blackwell. All rights reserved

Influence of parent material and soil use on arsenic forms in soils A case study in the Amblés Valley (Castilla-León, Spain)

2014

Abstract The total, water soluble and extractable concentrations with EDTA of As from topsoils from the Ambles Valley (Avila, Spain) were determined. The geochemical baseline concentrations of total As were established, and the relationships between the concentration of the different As forms and soil properties were investigated. Total As content in soils was related with parent material, whereas anthropogenic activities affected its mobility. Iron, aluminium, clay content, soil organic matter and soil pH were the main controlling factors for As soil concentrations. The geochemical baseline concentrations obtained (mg kg − 1 ) were 7.3–35 in soils on granite parent material and 2.2–6.8 in …

Background levels and baseline values of available heavy metals in Mediterranean greenhouse soils (Spain)

2011

Abstract This study determines extractable levels of Cd, Cu, Pb, Zn, Ni and Co in western Almeria (Spain) greenhouse surface soil horizons using EDTA solution, which is identified as the fraction available for organisms and plants. It also establishes background levels, geochemical baseline concentration and reference values (RV), and investigates the possible relationships between soil properties and elemental concentrations. The results show that the soil concentration of these extractable heavy metals was high as those reported by other authors for Spanish agricultural soils. The available RV concentrations obtained (mg kg −1 ) were: Cd 0.17, Cu 1.6, Pb 13.8, Zn 5.0 Ni 1.7 and Co 2.9. Us…

Age and tectonic setting of granitoid gneisses in the Eastern Desert of Egypt and south-west Sinai

1994

Strongly deformed and locally migmatized gneisses occur at several places in the southern Eastern Desert of Egypt and in Sinai and have variously been interpreted as a basement to Pan-african (≈900 to 600 Ma) supracrustal and intrusive assemblages. A suite of grabbroic to granitic gneisses was investigated in the Hafafit area, which constitutes an I-type calc-alkaline intrusive assemblage whose chemistry suggests emplacement along an active continental margin and whose granitoid members can be correlated with the so-called ‘Older Granites’ of Egypt. 207Pb/206Pb single zircon evaporation from three samples of the Hafafit gneisses yielded protolith emplacement ages between 677 ± 9 and 700 ± 1…

TEACHING TO USE IN SCIENCE AND MATHEMATICS PREVIOUSLY ACQUIRED SKILLS

2016

Goal of the research is to analyse how students currently succeed in using basic skills acquired in mathematics, in science and real-life contexts, before new reforms in Latvia for the development of students' competence have been started. Only about 20% of students in the national testing for 9th grade were able to do it. The research analysed students’ papers, results, and study aids, as well as teacher performance in lessons.

The pricing capability lifecycle of digital innovations

2021

The pricing capability provides a basis for firms’ competitive advantage. However, to date, the literature has not investigated how this capability forms, develops, and matures. In this study, we define the pricing capability as a dynamic capability consisting of three operational building blocks (tools and data, human and relational resources, and processes, routines, and mechanisms) as well as two dynamic capability building blocks (alertness and responsiveness, and value-based strategic thinking). Furthermore, we propose the pricing capability lifecycle of digital innovations that incorporates insights of the dynamic capabilities view (DCV). Based on a longitudinal multi-case study of fi…

In Situ U‐Th‐Pb Dating and Sr‐Nd Isotope Analysis of Bastnäsite by LA‐(MC)‐ICP‐MS

2019

Diseño y validación de la bater´ía de competencia literaria (BCL) Design and validation of the literary competence battery (LBC)

2019

El objetivo de este trabajo es presentar el proceso de diseño y validación de la Batería de Competencia Literaria (BCL). La Batería está formada por tres escalas: Escala de Conceptos Literarios (ECL), Escala de Procedimientos Literarios (EPL) y Escala de Actitudes hacia la literatura (EAL). La adquisición de la Competencia Literaria (CL) es el principal objetivo de la llamada Educación Literaria. Sin embargo, existen pocos métodos para medir esta competencia y la mayoría presentan problemas prácticos que limitan su estandarización. Un total de 221 adolescentes participaron en el proceso de diseño y validación. Se llevaron a cabo estudios sobre las propiedades de las pruebas incluyendo medid…

A Novel Energy Harvester for Powering Small UAVs: Performance Analysis, Model Validation and Flight Results

2019

The proposed work aims at exploring and developing new strategies to extend mission parameters (measured as travel distance and mission duration (MD)) of a new class of unmanned vehicles, named Micro Air Vehicles (MAVs). In this paper, a new analytical model, identifying all factors, which determine the MAV power consumption, is presented. Starting from the new model, the design of a nanoarray energy harvester, based on plasmonics nano-antenna technology is proposed. The preliminary study was based on a 22,066,058 22,066,058 &times