Search results for "PGD"
showing 6 items of 6 documents
Valor de los estudios cromosómicos en espermatozoides mediante la técnica de hibridación in situ fluorescente (FISH) en parejas estériles
2013
Los varones estériles presentan una frecuencia elevada de anomalías citogenéticas, algunas de ellas detectables mediante el cariotipo, pero otras limitadas a las células germinales como resultado de meiosis anormales. Con las nuevas técnicas de reproducción asistida como la Inyección Intracitoplásmica de Espermatozoides (ICSI), la fecundación por espermatozoides cromosómicamente anormales puede dar lugar a fallos de implantación, abortos de repetición y a la transmisión de anomalías cromosómicas a la descendencia. La hibridación in situ fluorescente (FISH) de espermatozoides es una técnica de análisis citogenético que, mediante el marcado de los cromosomas con moléculas de ADN fluorescentes…
Améliorer les effets anti-tumoraux des lymphocytes T folliculaires helper (Tfh) en ciblant la communication intercellulaire entre Tfh et Th2.
2019
It is now accepted that the immune system plays a critical role in cancers evolution (Hanahan et al., 2011). In this context, current understanding of the adaptive immune response made it a prime target. T CD4 cells, the main players of the adaptive immune system component, are known to possess distinct roles in the control of tumour growth. Thereby, Th2 and Tfh cells, both known to activate B cells in pathogenic infections, present antagonistic roles in cancer. Indeed, numerous studies demonstrate that Th2 cells are correlated with disease progression (especially via IL-4 secretion) (Koller et al., 2010 ; Roca et al., 2012), whereas Tfh cells are associated with a good prognosis for the pa…
Phosphoglycerate dehydrogenase genes differentially affect Arabidopsis metabolism and development.
2021
[EN] Unlike animals, plants possess diverse L-serine (Ser) biosynthetic pathways. One of them, the Phosphorylated Pathway of Serine Biosynthesis (PPSB) has been recently described as essential for embryo, pollen and root development, and required for ammonium and sulfur assimilation. The first and rate limiting step of PPSB is the reaction catalyzed by the enzyme phosphoglycerate dehydrogenase (PGDH). In Arabidopsis, the PGDH family consists of three genes, PGDH1, PGDH2 and PGDH3. PGDH1 is characterized as being the essential gene of the family. However, the biological significance of PGDH2 and PGDH3 remains unknown. In this manuscript, we have functionally characterized PGDH2 and PGDH3. Ph…
Prolonging in utero-like oxygenation after birth diminishes oxidative stress in the lung and brain of mice pups☆
2013
Background Fetal-to-neonatal transition is associated with oxidative stress. In preterm infants, immaturity of the antioxidant system favours supplemental oxygen-derived morbidity and mortality. Objectives To assess if prolonging in utero-like oxygenation during the fetal-to-neonatal transition limits oxidative stress in the lung and brain, improving postnatal adaptation of mice pups. Material and methods Inspiratory oxygen fraction (FiO2) in pregnant mice was reduced from 21% (room air) to 14% (hypoxia) 8–12 h prior to delivery and reset to 21% 6–8 h after birth. The control group was kept at 21% during the procedure. Reduced (GSH) and oxidized (GSSG) glutathione and its precursors [γ-glut…
Caracterización genética y funcional de la familia 3-Fosfoglicerato Deshidrogenasa (PGDH) de Arabidopsis thaliana
2013
El metabolismo primario de las plantas es un proceso complejo, en el que las vías que participan deben estar perfectamente coordinadas e integradas con el fin de lograr el desarrollo adecuado de las plantas y su aclimatación al medio ambiente. Un ejemplo de tal complejidad es la biosíntesis del aminoácido L- serina, que se lleva a cabo en al menos dos orgánulos diferentes y por rutas distintas. A pesar del papel crucial de la serina en las plantas, no se conoce cuál es la importancia biológica de la coexistencia de varias rutas biosintéticas; tampoco se conoce cómo interactúan estas rutas para mantener la homeostasis de dicho aminoácido en las células. Se han descrito tres rutas diferentes …
A Time Projection Chamber with GEM-Based Readout
2016
For the International Large Detector concept at the planned International Linear Collider, the use of time projection chambers (TPC) with micro-pattern gas detector readout as the main tracking detector is investigated. In this paper, results from a prototype TPC, placed in a 1 T solenoidal field and read out with three independent GEM-based readout modules, are reported. The TPC was exposed to a 6 GeV electron beam at the DESY II synchrotron. The efficiency for reconstructing hits, the measurement of the drift velocity, the space point resolution and the control of field inhomogeneities are presented.