Search results for "PHASE"

An integrated proteomic and metabolomic study to evaluate the effect of nucleus-cytoplasm interaction in a diploid citrus cybrid between sweet orange…

2018

Key message: Our results provide a comprehensive overview how the alloplasmic condition might lead to a significant improvement in citrus plant breeding, developing varieties more adaptable to a wide range of conditions. Abstract: Citrus cybrids resulting from somatic hybridization hold great potential in plant improvement. They represent effective products resulting from the transfer of organelle-encoded traits into cultivated varieties. In these cases, the plant coordinated array of physiological, biochemical, and molecular functions remains the result of integration among different signals, which derive from the compartmentalized genomes of nucleus, plastids and mitochondria. To dissect …

Toxicity of five Cry proteins against the insect pest Acanthoscelides obtectus (Coleoptera: Chrisomelidae: Bruchinae).

2019

Abstract The beetle Acanthoscelides obtectus (Say) causes severe post-harvest losses in the common bean (Phaseolus vulgaris). Under laboratory conditions, the susceptibility of A. obtectus to five coleopteran-specific Cry toxic proteins from Bacillus thuringiensis (Cry1Ba, Cry1Ia, Cry3Aa, Cry7Ab, and Cry23/37) was evaluated. After 30 days exposure, Cry proteins demonstrated high activity against A. obtectus adults (100% mortality). Proteins showed statistical differences in toxicity parameters compared to the control treatment, but the parameters were similar among them, and indicated that the final toxic effects can be observed after the 24th day. The toxic effects on A. obtectus larvae we…

Synchronicity in population systems: cause and consequence mixed

1999

DNA barcoding the phyllosoma of Scyllarides squammosus (H. Milne Edwards, 1837) (Decapoda: Achelata: Scyllaridae)

2016

Scyllarides has the largest number of species with commercial importance within the Scyllaridae family. As for other achelate lobsters, however, little is known of the unique long-lived planktonic phyllosoma stages of any of these tropical and temperate species. Recently, a large and diverse collection of Scyllaridae phyllosoma, compiled from cruises along the Coral Sea and spanning several years, has been analysed. Molecular evidence from DNA-barcoding and phylogenetic analyses is provided here on the identity of S. squammosus phyllosoma larvae, including stages that were previously undescribed or poorly known. As a consequence, the growth and morphological changes that occur during the mi…

Sex pheromone identified after solid phase microextraction from tergal glands of female alates in Cornitermes bequaerti (Isoptera, Nasutitermitinae)

2002

For the first time, a termite sex pheromone secreted by tergal glands has been isolated and identified. In the mandibulate nasute termite Cornitermes bequaerti, pairing of swarming imagoes is mediated by a sex pheromone secreted by females from their tergal glands. These well developed sexual glands located in front of tergites 8, 9, 10 are essentially composed of class 1 and 2 cells, but also of some glandular units of class 3 cells. The major volatile compound of tergal secretion was isolated by solid phase microextraction (SPME) and identified by GC-MS as (3Z, 6Z, 8E)-dodecatrien-1-ol. Sex attraction bioassays with synthetic (3Z, 6Z, 8E)-dodecatrien-1-ol showed that this alcohol was the …

Behavior of plant plasma membranes under hydrostatic pressure as monitored by fluorescent environment-sensitive probes.

2010

International audience; We monitored the behavior of plasma membrane (PM) isolated from tobacco cells (BY-2) under hydrostatic pressures up to 3.5 kbar at 30 °C, by steady-state fluorescence spectroscopy using the newly introduced environment-sensitive probe F2N12S and also Laurdan and di-4-ANEPPDHQ. The consequences of sterol depletion by methyl-β-cyclodextrin were also studied. We found that application of hydrostatic pressure led to a marked decrease of hydration as probed by F2N12S and to an increase of the generalized polarization excitation (GPex) of Laurdan. We observed that the hydration effect of sterol depletion was maximal between 1 and 1.5 kbar but was much less important at hig…

Dynamic Mathematical Modelling of the Removal of Hydrophilic VOCs by Biotrickling Filters

2015

A mathematical model for the simulation of the removal of hydrophilic compounds using biotrickling filtration was developed. The model takes into account that biotrickling filters operate by using an intermittent spraying pattern. During spraying periods, a mobile liquid phase was considered, while during non-spraying periods, a stagnant liquid phase was considered. The model was calibrated and validated with data from laboratory- and industrial-scale biotrickling filters. The laboratory experiments exhibited peaks of pollutants in the outlet of the biotrickling filter during spraying periods, while during non-spraying periods, near complete removal of the pollutant was achieved. The gaseou…

Water status and gas exchange of pistachio trees under different irrigation levels

2017

The aim of this study was to investigate the physiological responses of pistachio trees to varying water supply in the attempt to identify reliable parameters to manage irrigation. Three different irrigation regimes were applied to 25-year-old pistachio trees of cultivar 'Bianca' on P. terebinthus rootstock. Midday stem water potential (ΨSWP), leaf relative water content (RWC), light-saturated net CO2assimilation (An), stomatal conductance (gs), and ambient conditions were monitored throughout the season, and light response curves of net photosynthesis were constructed. To reflect tree water status, trees were separated into three classes of ΨSWP, no deficit (ΨSWP> -1.5 MPa), mild defici…

Preservation of viability and anti-Listeria activity of lactic acid bacteria, Lactococcus lactis and Lactobacillus paracasei, entrapped in gelling ma…

2015

In order to control undesirable microorganisms growth in foods, the performance of alginate and alginate-caseinate (an aqueous two-phase system) matrices entrapping lactic acid bacteria (LAB) (Lactobacillus paracasei LAB1 and Lactococcus lactis LAB3) was investigated. Polymeric matrices were initially loaded with Lcells at similar to 10(8-10) or similar to 10(4-6) CFU mL(-1), and were monitored, in liquid and gelled form (beads), for 12 days at 30 degrees C. In the liquid form, maximum cell density (similar to 10(9) CFU mL(-1)) was reached after 24 h whatever the matrix. Then, the Lpopulation decreased but remained higher in alginate-caseinate matrices: 10(7) and 10(6) CFU mL(-1) of LAB3 ce…

Drosophila cuticular hydrocarbons revisited : Mating status alters cuticular profiles

2010

Editor: Frederic Marion-Poll, INRA - Paris 6 - AgroParisTech, France.; International audience; Most living organisms use pheromones for inter-individual communication. In Drosophila melanogaster flies, several pheromones perceived either by contact/at a short distance (cuticular hydrocarbons, CHs), or at a longer distance (cis-vaccenyl acetate, cVA), affect courtship and mating behaviours. However, it has not previously been possible to precisely identify all potential pheromonal compounds and simultaneously monitor their variation on a time scale. To overcome this limitation, we combined Solid Phase Micro-Extraction with gas-chromatography coupled with mass-spectrometry. This allowed us (i…