Search results for "PLIF"
showing 10 items of 835 documents
Characterization of a cDNA encoding RP43, a CUB-domain-containing protein from the tube of Riftia pachyptila (Vestimentifera), and distribution of it…
2000
A major 43kDa protein from the protective tube of Riftiapachyptila (Vestimentifera), named RP43, was partly microsequenced after isolation by SDS/PAGE from the protein fraction of tubes collected around the hydrothermal vents at the East Pacific Rise. On the basis of the partial peptide sequences obtained, experiments using reverse-transcriptase-mediated PCR and rapid amplification of cDNA ends led to the complete cDNA sequence. Analysis of deduced amino acid sequence of RP43 showed the presence of CUB domains (100–110-residue-spanning domains first reported in the complement subcomponents C1r/C1s, epidermal-growth-factor-related sea urchin protein and bone morphogenetic protein 1) that se…
Molecular cloning of rat G-protein-coupled receptor kinase 6 (GRK6) from brain tissue, and its mRNA expression in different brain regions and periphe…
1997
The rat G-protein-coupled receptor kinase 6 (GRK6) cDNA was cloned from rat brain tissue by a combination of reverse-transcription polymerase chain reactions (RT-PCR), based on homology to the cloned human GRK6, and rapid amplification of cDNA ends (RACE-PCR). We obtained a clone of 2817 bp with an open reading frame of 1731 bp encoding for a protein of 576 amino acids that is 96.7% identical and 97.9% similar to its human counterpart. mRNA was detectable in all brain areas examined. In addition, GRK6 was expressed in skeletal muscle, small intestine, aorta, liver, heart, lung, thymus, stomach, uterus and kidney.
An AFLP clock for the absolute dating of shallow-time evolutionary history based on the intraspecific divergence of southwestern European alpine plan…
2009
The dating of recent events in the history of organisms needs divergence rates based on molecular fingerprint markers. Here, we used amplified fragment length polymorphisms (AFLPs) of three distantly related alpine plant species co-occurring in the Spanish Sierra Nevada, the Pyrenees and the southwestern Alps/Massif Central to establish divergence rates. Within each of these species (Gentiana alpina, Kernera saxatilis and Silene rupestris), we found that the degree of AFLP divergence (D(N72)) between mountain phylogroups was significantly correlated with their time of divergence (as inferred from palaeoclimatic/palynological data), indicating constant AFLP divergence rates. As these rates d…
Differential cycles of range contraction and expansion in European high mountain plants during the Late Quaternary: insights from Pritzelago alpina (…
2003
Nuclear DNA sequence variation of the internal transcribed spacer (ITS) and amplified fragment length polymorphisms (AFLPs) were used to illuminate the evolutionary history of Pritzelago alpina, a herbaceous perennial of (sub)alpine to nival habitats of the European high mountains. Maximum likelihood analysis of ITS sequences of P. alpina, Hornungia petraea and Hymenolobus procumbens (the 'Pritzelago alliance') resolved P. alpina and H. petraea as sister taxa. ITS divergence estimates support an origin for P. alpina in the Late Tertiary, while intraspecific diversification started in the Late Quaternary (0.4-0.9 million years ago). AFLP analysis of 76 individuals of P. alpina, representing …
Proposal of Up-to-Date Standards on Methods of Measuring Noise Parameters of Microwave Transistors
1994
One of the most interesting topics for microrwave community is the characterization of low-noise transistors. After so many years, the Standards suggested by IEEE in 1960 are considered obsolete by the experimenters. A new methodology is here proposed as a standard. To support this proposal, an original measuring system for the complete characterization of microwave transistors in terms of noise, gain and scattering parameters from noise figure measurements only is presented.
Comparison between complete and simplified methods for determining the microwave noise parameters of HEMTs
1994
Modem automatic noise parameter measuring systems are based on measurements of some values of F(rs ) for different values of IГs (more than four, for redundancy) realized by adjusting an admittance transformer (tuner) inserted between the noise source and the TUT mput . Such values are then manipulated by means of proper data processing techniques using eqn.( 1) [ 11. This measuring procedure is hereafter referred to as standard to point out that the old `Standards on methods of measuring noise in linear twoports´ published by the IRE Subcommittee on noise in 1960 is considered obsolete by all the experimenters.
WDM switching employing a hybrid silicon-plasmonic A-MZI
2012
We demonstrate a system-level evaluation of an A-MZI with 60μm long DLSPP active branches exhibiting more than 14dB extinction ratio. Error-free switching operation is achieved for a 4×10Gb/s incoming WDM data stream with only 13.1mW power consumption.
A brief history of the formation of DNA databases in forensic science within Europe.
2001
The introduction of DNA analysis to forensic science brought with it a number of choices for analysis, not all of which were compatible. As laboratories throughout Europe were eager to use the new technology different systems became routine in different laboratories and consequently, there was no basis for the exchange of results. A period of co-operation then started in which a nucleus of forensic scientists agreed on an uniform system. This collaboration spread to incorporate most of the established forensic science laboratories in Europe and continued through two major changes in the technology. At each step agreement was reached on which systems to use. From the beginning it was realise…
A sentence based system for measuring syntax complexity using a recurrent deep neural network
2018
In this paper we present a deep neural network model capable of inducing the rules that identify the syntax complexity of an Italian sentence. Our system, beyond the ability of choosing if a sentence needs of simplification, gives a score that represent the confidence of the model during the process of decision making which could be representative of the sentence complexity. Experiments have been carried out on one public corpus created specifically for the problem of text-simplification.
A recurrent deep neural network model to measure sentence complexity for the Italian Language
2019
Text simplification (TS) is a natural language processing task devoted to the modification of a text in such a way that the grammar and structure of the phrases is greatly simplified, preserving the underlying meaning and information contents. In this paper we give a contribution to the TS field presenting a deep neural network model able to detect the complexity of italian sentences. In particular, the system gives a score to an input text that identifies the confidence level during the decision making process and that could be interpreted as a measure of the sentence complexity. Experiments have been carried out on one public corpus of Italian texts created specifically for the task of TS…