Search results for "PORIN"
showing 10 items of 260 documents
Tissue expression of the vesicle protein pantophysin
1999
The cell-type restricted expression of cytoplasmic microvesicle membrane protein isoforms may be a consequence of the functional adaptation of these vesicles to the execution of specialized processes in cells of different specialization. To characterize the expression of the vesicle protein pantophysin, an isoform of the synaptic vesicle proteins synaptophysin and synaptoporin, we have prepared and characterized antibodies useful for the immunological detection of pantophysin in vitro and in situ. Using these reagents, we show by immunoblot analyses that pantophysin expression is not homogeneous but differs significantly between various bovine tissues. Furthermore, these differences are not…
Insertion of a malE B-Galactosidase fusion protein into the envelope of Escherichia coli disrupts biogenesis of outer membrane proteins and processin…
1982
The synthesis of a membrane-bound MalE ,B-galactosidase hybrid protein, when induced by growth of Escherichia coli on maltose, leads to inhibition of cell division and eventually a reduced rate of mass increase. In addition, the relative rate of synthesis of outer membrane proteins, but not that of inner membrane proteins, was reduced by about 50%o. Kinetic experiments demonstrated that this reduction coincided with the period of maximum synthesis of the hybrid protein (and another maltose-inducible protein, LamB). The accumulation of this abnormal protein in the envelope therefore appeared specifically to inhibit the synthesis, the assembly of outer membrane proteins, or both, indicating t…
Extended-Spectrum ß-Lactamase, AmpC-Producing, and Fluoroquinolone-Resistant Escherichia coli in Retail Broiler Chicken Meat, Italy.
2015
Background: Globally, antimicrobial drug-resistant Escherichia coli is among the most common etiological agents of invasive disease in humans. In Europe, increasing proportions of infections due to third-generation cephalosporins and/or fluoroquinolone-resistant extraintestinal pathogenic E. coli (ExPEC) strains are reported. E. coli from poultry are those more closely linked to human E. coli, but lack of reliable data makes it difficult to assess the attributable risk of different food sources. In the present study, our objective was to investigate the antimicrobial resistance profile, phylogenetic background, and virulence factors of E. coli isolates from broiler chicken meat sold at reta…
Possible coumarin-like mechanism of action for cephalosporins.
1984
In three patients treated with cephalosporins (one patient with latamoxef, two patients with cefazedone) vitamin K1 was injected to investigate whether this was followed by an increase in vitamin K1 2,3-epoxide plasma concentrations as compared to controls. Such a rise in K1-epoxide concentrations in the plasma can be demonstrated following treatment with coumarins. This reflects an inhibition of the vitamin K1-epoxide reductase in the liver. Coumarins are thought to induce hypoprothrombinaemia by such a mechanism. In all three patients we found a considerable increase in the vitamin K1-epoxide plasma concentrations following injection of 10 mg vitamin K1, whereas in normal subjects only tr…
The syndrome "basses richesses" of sugar beet in France is associated with different pathogen types and insect vectors
2007
International audience; The syndrome “basses richesses” (SBR) of sugar beet in France is associated with two phloem-restricted uncultivable bacteria: a stolbur phytoplasma and a y-3 proteobacteria. The known vector of proteobacteria is a cixiid planthopper, Pentastiridius leporinus (Hemiptera Cixiidae), formerly shown to transmit both the prokaryotes. The role of P. leporinus and of two other planthopper species, Cixius wagneri and Hyalesthes obsoletus, in spreading the two pathogens to sugar beet were compared and quantified. Because of its abundance and high infection rates with proteobacterium, P. leporinus was confirmed to be the economic vector of SBR disease. P. leporinus and C. wagne…
Cytoplasmic Parvovirus Capsids Recruit Importin Beta for Nuclear Delivery
2019
Parvoviruses are an important platform for gene and cancer therapy. Their cell entry and the following steps, including nuclear import, are inefficient, limiting their use in therapeutic applications. Two models exist on parvoviral nuclear entry: the classical import of the viral capsid using nuclear transport receptors of the importin (karyopherin) family or the direct attachment of the capsid to the nuclear pore complex leading to the local disintegration of the nuclear envelope. Here, by laser scanning confocal microscopy and in situ proximity ligation analyses combined with coimmunoprecipitation, we show that infection requires importin β-mediated access to the nuclear pore complex and …
Clinical and MRI measures to identify non-acute MOG-antibody disease in adults
2022
Abstract MRI and clinical features of myelin oligodendrocyte glycoprotein (MOG)-antibody disease may overlap with those of other inflammatory demyelinating conditions posing diagnostic challenges, especially in non-acute phases and when serologic testing for MOG antibodies is unavailable or shows uncertain results. We aimed to identify MRI and clinical markers that differentiate non-acute MOG-antibody disease from aquaporin 4 (AQP4)-antibody neuromyelitis optica spectrum disorder and relapsing remitting multiple sclerosis, guiding in the identification of patients with MOG-antibody disease in clinical practice. In this cross-sectional retrospective study, data from 16 MAGNIMS centres were i…
Comparing tacrolimus ointment and oral cyclosporine in adult patients affected by atopic dermatitis: a randomized study
2004
BACKGROUND: Atopic dermatitis (AD) is a chronic allergic inflammatory disease, which manifests itself with eczematous skin lesions. OBJECTIVE: We compared the clinical efficacy of tacrolimus ointment (0.1%) given twice a day and oral cyclosporine (3 mg/kg) given once daily. Rescue medication for itching included cetirizine 10-20 mg (equal to one or two tables). METHODS: Thirty patients, aged 13-45 years (mean+/-SD 27.1+/-10.9), with a history of moderate-to-severe AD were randomized to treatments, 15 patients for each treatments. Assessment of efficacy was based on SCORAD, on scores of daily itching, erythema, interference with sleep, due to the skin condition and days without use of cetiri…
Les souches d'E. coli résistantes aux antibiotiques
2016
National audience; Le projet "CIREC" vise à étudier la prévalence et la caractérisation des souches d’E.coli productrices de β-lactamase à spectre élargi (BLSE) de type CTX-M dans les eaux usées et les boues issues d’une quarantaine de stations d’épuration (STEP) de la région Bourgogne. Puis, il s’agira d’identifier et caractériser les voies possibles de transfert de ces souches, les rejets d’effluents traités des STEP dans les rivières pouvant avoir des conséquences sur la contamination de la faune aquatique d’eau douce.
Les souches d’Escherichia coli résistantes aux antibiotiques
2017
Parmi les bactéries multi-résistantes, les entérobactéries productrices de β-lactamase à spectre élargi (BLSE) occupent une place croissante. Parmi celles-ci, c’est Escherichia coli (entérobactérie commensale du tube digestif de l’homme et de l’animal) qui est l’espèce la plus concernée par cette production enzymatique. Cependant, il existe peu de données sur l’existence de réservoirs environnementaux pour ces souches, leur capacité à survivre dans l’environnement et en particulier dans les eaux douces et dans les sols. Le projet "CIREC" visait donc à étudier la prévalence et la caractérisation des souches d’E.coli productrices de BLSE de type CTX-M dans les eaux usées et les boues issues d…