Search results for "PROTEINS"

Main protein component of high density lipoprotein, apolipoprotein A1, modulates leukocyte cell respiration

2015

HDL:n (high density lipoprotein) pääproteiinikomponentin, apolipoproteiini A1:n (ApoA1), on todettu lisäävän glykolyysia ja oksidatiivista fosforylaatiota hiiren luurankolihassoluissa. ApoA1:llä oletetaan myös olevan merkittävä rooli lihaksen normaalissa aineenvaihdunnassa ja siihen liittyvissä sairauksissa. ApoA1 vaikuttaa leukosyyttien aktivaatioon ja jakautumiseen säätelemällä solun sisäistä kolesterolitasoa. Nämä muutokset vaikuttavat myös solun aineenvaihduntaan. ApoA1:n suoranaista vaikutusta leukosyyttien soluhengitykseen ei kuitenkaan ole aiemmin tutkittu. Aiemmin on esitetty, että leukosyyttejä voitaisiin käyttää biomarkkerina kuvaamaan muutoksia mitokondrioiden toiminnassa aineenv…

The role of lipid metabolism in the acquisition of desiccation tolerance inCraterostigma plantagineum: a comparative approach

2013

Summary Dehydration leads to different physiological and biochemical responses in plants. We analysed the lipid composition and the expression of genes involved in lipid biosynthesis in the desiccation-tolerant plant Craterostigma plantagineum. A comparative approach was carried out with Lindernia brevidens (desiccation tolerant) and two desiccation-sensitive species, Lindernia subracemosa and Arabidopsis thaliana. In C. plantagineum the total lipid content remained constant while the lipid composition underwent major changes during desiccation. The most prominent change was the removal of monogalactosyldiacylglycerol (MGDG) from the thylakoids. Analysis of molecular species composition rev…

In-Depth Characterization of Bioactive Extracts from Posidonia oceanica Waste Biomass

2019

© 2019 by the authors.

CYGD: the Comprehensive Yeast Genome Database.

2005

The comprehensive resource is available under http://mips.gsf.de/genre/proj/yeast/.; International audience; The Comprehensive Yeast Genome Database (CYGD) compiles a comprehensive data resource for information on the cellular functions of the yeast Saccharomyces cerevisiae and related species, chosen as the best understood model organism for eukaryotes. The database serves as a common resource generated by a European consortium, going beyond the provision of sequence information and functional annotations on individual genes and proteins. In addition, it provides information on the physical and functional interactions among proteins as well as other genetic elements. These cellular network…

What I say is not necessary what I do: pulses consumption in French adults

2018

International audience; The consumption of pulses in the human diet is considered to have several advantages: from a nutritional point of view, pulses are rich in dietary fibers, complex carbohydrate, minerals/vitamins and an important source of vegetal proteins. They are considered essential to insure food security and support environmental sustainability. Despite these benefits, the consumption of pulses in France has declined over the past decades, dropping from 7.2 kg in 1920 to 1.7 kg/ person per year in 2016. The aim of this study is to understand the representation of pulses among French non-vegetarian consumers, using a direct method (questionnaire) and an indirect method (scenario …

Kinetic evidence for the incorporation of the [(pentamethylcyclopentadienyl) (2,2′-bipyridyl)(aquo)rhodium(III)] complex into DPPC vesicles

2008

Abstract The binding of the [(pentamethylcyclopentadienyl) (2,2′-bipyridyl)(aquo)rhodium(III)] complex [Cp*RhIII(bpy)H2O]2+, to l -α-dipalmitoylphosphatidyl choline (DPPC) vesicles has been estimated by studying the kinetics of the electron transfer reaction between the rhodium(III) complex and formiate ions. Kinetic measurements carried out under anaerobic conditions in absence and presence of DPPC show that the total reaction is composed of two steps. The rate of the first reaction increases with the phospholipid concentration, while that of the second process is independent of the concentration of DPPC. This is consistent with a reaction, where the two reacting species are partitioned be…

An ancient virus type from extreme environments

2014

Microtubules and microfilaments in HSV-Infected rabbit-kidney cells.

1981

In rabbit kidney cells infected with strains of Herpes simplex virus producing either cell-rounding or polycaryocytosis. Vinblastine induced paracrystals. This could be shown by phase-contrast- and electron-microscopy. Infections were done under one-step-growth conditions or at low MOI. 90 per cent noninfected cells contained stress fibers as detected by Servablue R250-staining. Shortly after recruitment into polycaryocytes, stress fibres of normal length appearing in criss-cross arrangement can be seen in the periphery of these cells. Later they polymerize to very long fibers and finally they are partially destroyed. The time of destruction depends on the MOI employed. By using Actinomycin…

Peptide-mediated interference with baculovirus transduction

2007

Baculovirus represents a multifunctional platform with potential for biomedical applications including disease therapies. The importance of F3, a tumor-homing peptide, in baculovirus transduction was previously recognized by the ability of F3 to augment viral binding and gene delivery to human cancer cells following display on the viral envelope. Here, F3 was utilized as a molecular tool to expand understanding of the poorly characterized baculovirus-mammalian cell interactions. Baculovirus-mediated transduction of HepG2 hepatocarcinoma cells was strongly inhibited by coincubating the virus with synthetic F3 or following incorporation of F3 into viral nucleocapsid by genetic engineering, th…

Occlusion-derived baculovirus: interaction with human cells and evaluation of the envelope protein P74 as a surface display platform.

2008

To develop complementary baculovirus-based tools for gene delivery and display technologies, the interaction of occlusion-derived baculovirus (ODV) with human cells, and the functionality of the P74 ODV envelope protein for display of the IgG-binding Z domains (ZZP74) were evaluated. The cellular binding of ODV was concentration-dependent and saturable. Only minority of the bound virions were internalized at both 37 and 4 degrees C, suggesting usage of direct membrane fusion as the entry mode. The intracellular transport of ODV was confined in vesicular structures peripheral to the plasma membrane, impeding subsequent nuclear entry and transgene expression. Transduction of ODV was not rescu…