Search results for "PSO"
showing 10 items of 679 documents
NF-κB and STAT3 Inhibition as a Therapeutic Strategy in Psoriasis: In Vitro and In Vivo Effects of BTH
2013
Benzo[b]thiophen-2-yl-3-bromo-5-hydroxy-5H-furan-2-one (BTH) is a simple and interesting synthetic derivative of petrosaspongiolide M, a natural compound isolated from a sea sponge with demonstrated potent anti-inflammatory activity through inhibition of the NF-κB signaling pathway. In the present study, we report the in vitro and in vivo pharmacological effect of BTH on some parameters related to the innate and adaptive response in the pathogenesis of psoriasis. BTH inhibited the release of some of the key psoriatic cytokines such as tumor necrosis factor α, IL-8, IL-6, and CCL27 through the downregulation of NF-κB in normal human keratinocytes. Moreover, it impaired signal transducers and…
Potential antipsoriatic effect of chondroitin sulfate through inhibition of NF-κB and STAT3 in human keratinocytes
2012
Abstract Chondroitin sulfate (CS) is a natural glycosaminoglycan, formed by the 1–3 linkage of d -glucuronic acid to N-acetylgalactosamine, present in the extracellular matrix. It is used as a slow acting disease modifying agent in the treatment of osteoarthritis, and part of its beneficial effects are due to its antiinflammatory properties that result from an inhibitory effect on NF-κB signaling pathway. This ability raises the hypothesis that CS might be effective in other chronic inflammatory processes such as psoriasis, in which a deregulation of NF-κB is a key feature. In addition, psoriasis is characterized by an upregulation of STAT3 signaling pathway that is related to the epidermal…
Studies of Jak/STAT3 expression and signalling in psoriasis identifies STAT3-Ser727 phosphorylation as a modulator of transcriptional activity
2013
Jak/Tyk proteins have recently aroused as possible therapeutic targets for the treatment of psoriasis. In psoriasis, these proteins signal through STAT molecules including STAT3, and STAT3 expression and activation has been shown augmented in psoriatic lesions. Here, we characterized the expression of Jak/Tyk proteins in lesional compared with non-lesional psoriatic skin. Jak1, Jak2 mRNA and protein and Tyk2 mRNA appeared to be downregulated, whereas Jak3 mRNA expression was increased. Moreover, STAT3 expression and activation was examined in psoriasis. STAT3 is activated at two phosphorylation sites: Tyr705 and Ser727. Both phosphorylation sites were phosphorylated in lesional psoriatic sk…
Avarol inhibits TNF-a generation and NF-kB activation in human cells and in animal models
2007
Avarol is a marine sesquiterpenoid hydroquinone with interesting pharmacological properties including anti-inflammatory and antipsoriatic effects. In the present study we evaluated the pharmacological effect of avarol on some inflammatory parameters related to the pathogenesis of psoriasis. Avarol inhibited tumor necrosis factor-alpha (TNF-alpha) generation in stimulated human monocytes (IC(50) 1 microM) and TNF-alpha-induced activation of nuclear factor-kappaB (NF-kappaB)-DNA binding in keratinocytes. In the mouse air pouch model, administration of avarol produced a dose-dependent reduction of TNF-alpha generation (ED(50) 9.2 nmol/pouch) as well as of interleukin (IL)-1beta, prostaglandin …
The family of furocoumarins: Looking for the best photosensitizer for phototherapy
2008
Abstract Furocoumarins are widely used as photosensitizers in photochemical therapies against different skin disorders such as psoriasis and vitiligo. Absorption of near-UV light by the chromophore triggers a set of photoreactions related to the therapeutic properties of the technique: linkage of a furocoumarin to thymine DNA nucleobases preventing proliferation of pathogenic cells, or generation of highly reactive singlet oxygen in damaged tissues. The family of furocoumarins has been studied in depth for many years seeking a drug having the most remarkable set of properties to act as a photosensitizer. For this purpose, understanding the underlying photochemical mechanisms behind the effe…
STAT1 expression and activation is increased in lesional psoriatic skin
2013
Summary Background The JAK (Janus kinase)/STAT (signal transducer and activator of transcription) signalling pathway is known to play an important role in many cellular processes including inflammation. The activation of STAT1 is dependent on tyrosine 701 and serine 727 phosphorylation, which leads to the formation of the STAT dimer and modulation of STAT1 activity, respectively. Objective To determine STAT1 expression and activation in psoriatic skin. Methods Biopsies were collected from patients with psoriasis. mRNA expression was evaluated by quantitative polymerase chain reaction, whereas the protein and phosphorylation level of STAT1 were evaluated by Western blotting. STAT1 localiz…
Huumeet ja kirjailijoiden luovuus
2017
Pro gradu -tutkielmani käsittelee huumeiden ja kirjailijoiden luovuuden välistä yhteyttä. Kirjailijat ja alkoholi yhdistetään helposti toisiinsa, ja erityisesti siten, että alkoholi on ollut muusa. Alkoholiin mieltyneiden kirjailijoiden lisäksi historia tuntee myös huumeita käyttäneitä kirjailijoita. Miten huumeet vaikuttivat heidän luovuuteensa? Oliko eri huumeilla erilaisia vaikutuksia? Vai oliko huumeista pikemminkin haittaa luovalle työlle? Tarkastelen neljän hyvin erilaisen kirjailijan päihteidenkäyttöhistoriaa ja tuotantoa. Kirjailijat ovat William S. Burroughs, Jack Kerouac, Hunter S. Thompson ja Stephen King. Heistä vain viimeksi mainittu on vielä elossa. Otan myös huomioon kirjaili…
Tissue kallikrein and kininogen in human sweat glands and psoriatic skin
1991
The cellular localization of immunoreactive tissue kallikrein and kininogen was studied in normal and psoriatic human skin. Immunoreactivity to both enzyme and substrate was observed in secretory granules of the dark cells in the secretory fundus (acinus) of the sweat glands. Double immunostaining revealed a segmental distribution of the two antigens. Each acinar section contained either tissue kallikrein or kininogen. However, there appeared to be a junctional zone in which both were present, but in separate dark cells. Immunoreactivity for both antigens was also observed in close apposition to the luminal microvilli of the duct cells. No specific immunostaining was seen in sebaceous gland…
Kontaktdermatītu raksturojums pacientiem ar psoriāzi
2022
Dermatoloģijas praksē ir svarīgi noskaidrot iespējamos kontaktalergēnus, jo tas ļauj uzlabot pacienta dzīves kvalitāti. Metode, ar kuras palīdzību nosaka kontaktalergēnus, ir vienkārša, pieejama un pacientam nesāpīga. Pacientiem, kuriem ir diagnosticēta psoriāze, ir īpaši ieteicams veikt šādu testu, jo ādas barjerfunkcija ir jau traucēta un tas var palīdzēt izvairīties no lielākiem ādas bojājumiem, kas pasliktinātu dzīves kvalitāti. Par darba mērķis tika izvirzīts, izmantojot aplikācijas testus, noskaidrot biežākos kontaktalergēnus pacientiem ar un bez diagnozes psoriasis vulgaris un vai ir atšķirība starp kontaktalergēniem abās pacientu grupās. Šai nolūkā tika veikts anonimizēts, retrospek…
Variance estimation and asymptotic confidence bands for the mean estimator of sampled functional data with high entropy unequal probability sampling …
2013
For fixed size sampling designs with high entropy it is well known that the variance of the Horvitz-Thompson estimator can be approximated by the H\'ajek formula. The interest of this asymptotic variance approximation is that it only involves the first order inclusion probabilities of the statistical units. We extend this variance formula when the variable under study is functional and we prove, under general conditions on the regularity of the individual trajectories and the sampling design, that we can get a uniformly convergent estimator of the variance function of the Horvitz-Thompson estimator of the mean function. Rates of convergence to the true variance function are given for the re…