Search results for "PTO"
showing 10 items of 28599 documents
Tobacco cells contain a protein, immunologically related to the neutrophil small G protein Rac2 and involved in elicitor-induced oxidative burst.
1997
Abstract Suspension-cultured cells of Nicotiana tabacum generated active oxygen species (AOS) when they were treated with the proteinaceous elicitor, cryptogein. This response was blocked by diphenylene iodonium, an inhibitor of the neutrophil NADPH oxidase. When microsomal extracts of tobacco cells were probed with an antibody directed against the human small G protein Rac2, two immunoreactive proteins were detected at 18.5 and 20.5 kDa. The same experiment performed with cytosolic extracts of tobacco cells led to the observation of a strong immunoreactive protein at 21.5 kDa only in the cryptogein-treated cells. The appearance of this cytosolic protein was related to the production of AOS…
The combined action of 9 lipoxygenase and galactolipase is sufficient to bring about programmed cell death during tobacco hypersensitive response
2005
International audience; Oxylipins, derived from fatty acid hydroperoxides (FAHs), are thought to play different roles during plant pathogen interactions. During hypersensitive response (HR) some of them serve as signals necessary for defence gene activation whereas others could contribute to pathogen killing or could participate in the execution of plant programmed cell death (PCD) associated with this resistance. In order to address the role of these compounds in the latter process, we have closely observed lipid peroxidation, the first step of this metabolic pathway, under different situations which led either to accelerated or inhibited HR cell death. The oxidative process has been studi…
Type-2 histone deacetylases as new regulators of elicitor-induced cell death in plants
2011
voir Addenda, notes additionnelles complétant l'article : "Dahan, J., Hammoudi, V., Wendehenne, D., Bourque, S. (2011). Type 2 histone deacetylases play a major role in the control of elicitor-induced cell death in tobacco. Plant signaling & behavior, 6 (11), 1865-1867. DOI : 10.4161/psb.6.11.17848".; International audience; Plant resistance to pathogen attack is often associated with a localized programmed cell death called hypersensitive response (HR). How this cell death is controlled remains largely unknown. Upon treatment with cryptogein, an elicitor of tobacco defence and cell death, we identified NtHD2a and NtHD2b, two redundant isoforms of type-2 nuclear histone deacetylases (HDACs…
Hydrogen peroxide induces programmed cell death features in cultured tobacco BY-2 cells, in a dose-dependent manner
2001
Active oxygen species (AOS), especially hydrogen peroxide, play a critical role in the defence of plants against invading pathogens and in the hypersensitive response (HR). This is characterized by the induction of a massive production of AOS and the rapid appearance of necrotic lesions is considered as a programmed cell death (PCD) process during which a limited number of cells die at the site of infection. This work was aimed at investigating the mode of cell death observed in cultures of BY-2 tobacco cells exposed to H(2)O(2). It was shown that H(2)O(2) is able to induce various morphological cell death features in cultured tobacco BY-2 cells. The hallmarks of cell death observed with fl…
Involvement of putative glutamate receptors in plant defence signaling and NO production
2011
International audience; Ionotropic glutamate receptors (iGluRs) are non-selective cation channels permeable to calcium, present in animals and plants. In mammals, glutamate is a well-known neurotransmitter and recently has been recognized as an immunomodulator. As animals and plants share common mechanisms that govern innate immunity with calcium playing a key role in plant defence activation, we have checked the involvement of putative iGluRs in plant defence signaling. Using tobacco cells, we first provide evidence supporting the activity of iGluRs as calcium channels and their involvement in NO production as reported in animals. Thereafter, iGluRs were shown to be activated in response t…
Maternal effects in quail and zebra finches: behavior and hormones.
2013
8 pages; International audience; Maternal effects are influences of parents on offspring phenotype occurring through pathways other than inherited DNA. In birds, two important routes for such transmission are parental behavior and non-DNA egg constituents such as yolk hormones. Offspring traits subject to parental effects include behavior and endocrine function. Research from the Adkins-Regan lab has used three avian species to investigate maternal effects related to hormones and behavior. Experiments with chickens and Japanese quail have shown that maternal sex steroids can influence sex determination to produce biased offspring sex ratios. Because all birds have a ZZ/ZW chromosomal sex de…
Role of lignin and sodium carbonate on the swelling behavior of black liquor droplets during combustion
2018
AbstractA partial removal of lignin from black liquor (BL) by carbonation and lignin precipitation was studied. In lignin-lean BL droplets during combustion in a laboratory furnace at 800°C in stagnant air, the maximum swelling was decreased. This observation was interpreted as showing that the lignin content decrement is due mainly to removal of higher molar mass (HMM) lignin and that the Na2CO3content of the BL is increased. Stepwise precipitation experiments with industrial softwood and hardwood kraft BLs by carbonation (resulting in pH decrement from 13 to 9) indicated that a fraction of HMM lignin (MM >10 kDa) with a higher amount of carbohydrates precipitated more prominently and e…
High-speed duetting-latency times of the female acoustic response within the bush-cricket genera Leptophyes and Andreiniimon (Orthoptera, Phaneropter…
2018
To find a mate, male and female bush-crickets of the family Phaneropteridae typically engage in duets. The male sings and the female responds. For mutual recognition, the amplitude pattern of the male song and the species-specific timing of the female response have been shown to be very important. In the seven studied species, belonging to the generaLeptophyesandAndreiniimon, these duets are extremely fast and nearly completely in the ultrasonic range. The females produce very short sounds by fast closing movements of the tegmina. They respond with species-specific delays of 20 to 150 ms after the beginning of the male song. The different latency times are probably not important for species…
Ultrastructure of regions containing homologous loci in polytene chromosomes of Drosophila melanogaster and Drosophila subobscura.
1998
We have used a new approach involving in situ hybridisation and electron microscopy to establish ultrastructural homologies between polytene chromosome regions of Drosophila melanogaster and Drosophila subobscura. Twelve probes were chosen to cover all the chromosomal elements: the myospheroid gene, the collagen type IV gene, the collagen-like gene, the w26 homeobox gene, the beta3 tubulin gene, the kinesin heavy chain gene, the tryptophan hydrolase gene, the Hsp82, Hsp22-26 and Hsp23-28, Hsp68, Hsp70 genes and the beta unit of the F0-F1 ATPase gene. Most of these loci were previously undescribed in D. subobscura and imprecisely located in D. melanogaster. We have demonstrated here, by an u…
European Vegetation Archive (EVA): an integrated database of European vegetation plots
2015
Biurrun, Idoia/0000-0002-1454-0433; Rojo, Maria Pilar Rodriguez/0000-0001-5449-9386; Ermakov, Nikolai/0000-0001-7550-990X; De Sanctis, Michele/0000-0002-7280-6199; Svenning, Jens-Christian/0000-0002-3415-0862; Virtanen, Risto/0000-0002-8295-8217; Agrillo, Emiliano/0000-0003-2346-8346; Onyshchenko, Viktor/0000-0001-9079-7241; Marceno, Corrado/0000-0003-4361-5200; Willner, Wolfgang/0000-0003-1591-8386; Fernandez-Gonzalez, Federico/0000-0003-1234-4065; Jansen, Florian/0000-0002-0331-5185; Swacha, Grzegorz/0000-0002-6380-2954; Dengler, Jurgen/0000-0003-3221-660X; Guarino, Riccardo/0000-0003-0106-9416; Sopotlieva, Desislava/0000-0002-9281-7039; Venanzoni, Roberto/0000-0002-7768-0468; Chytry, Mil…