Search results for "Paper"

Reductive modification of genetically encoded 3-nitrotyrosine sites in alpha synuclein expressed in E.coli

2019

Tyrosine nitration is a post-translational protein modification relevant to various pathophysiological processes. Chemical nitration procedures have been used to generate and study nitrated proteins, but these methods regularly lead to modifications at other amino acid residues. A novel strategy employs a genetic code modification that allows incorporation of 3-nitrotyrosine (3-NT) during ribosomal protein synthesis to generate a recombinant protein with defined 3-NT-sites, in the absence of other post-translational modifications. This approach was applied to study the generation and stability of the 3-NT moiety in recombinant proteins produced in E.coli. Nitrated alpha-synuclein (ASYN) was…

Survey of Italian Pediatricians on awareness, experiences and beliefs regarding direct-to-consumer genetic testing in minors

2022


 Background: Our study wanted to assess Italian pediatricians’ awareness, experience and beliefs regarding directto-consumer (DTC) genetic tests (GT) in minors, with a focus on those for predisposition to complex disease, lyfestyle, athletic ability and other inborn talents.
 Methods: A 28-item questionnaire was administered through the SurveyMonkey® web platform to the 9,086 members of the Italian Society of Pediatrics for which a valid email address was available. The survey was opened from April through November 2017. Statistical analyses were performed using the Graphpad software package.
 Results: 36.2% of the 442 respondents were aware of DTC-GT, but only 23.1% of them…

The dynamics of expertise acquisition in sport: The role of affective learning design

2015

Objectives: The aim of this position paper is to discuss the role of affect in designing learning experiences to enhance expertise acquisition in sport. The design of learning environments and athlete development programmes are predicated on the successful sampling and simulation of competitive performance conditions during practice. This premise is captured by the concept of representative learning design, founded on an ecological dynamics approach to developing skill in sport, and based on the individual-environment relationship. In this paper we discuss how the effective development of expertise in sport could be enhanced by the consideration of affective constraints in the representativ…

Vascularized composite allotransplantation - a Council of Europe position paper

2017

After more than 120 hand-upper extremity and 37 face transplant procedures performed worldwide, vascularized composite allotransplantation (VCA) now falls under the scope of organ transplant legislation in Europe and the United States. While in the USA, VCA has been considered as standard care since 2014, VCA in Europe is still performed through clinical research trials, except in United Kingdom. However, after two decades of favourable experience with upper extremity transplantation (UET), professionals in Europe are proposing hand allotransplantation as “controlled standard” care, as opposed to face transplantation (FT), which is still a challenging activity. The European Committee on Org…

Il diritto penale oltre la frontiera

2023

A partire dalla considerazione del ruolo che il principio di territorialità riveste nel diritto penale, il contributo sviluppa una rapida riflessione sui rapporti tra cosmopolitismo giuridico e diritto penale e si interroga sui possibili influssi che le teorie sul fondamento universalistico dei diritti umani possono esercitare sul sistema penale stato-centrico.

The use of calcined paper mill sludge as a chemical precipitant in the simultaneous removal of ammonium and phosphate : paper mill waste recycling an…

2020

Currently, recycling and re-use of materials is extremely important due to the diminishing of natural resources. The objective of the European Union’s circular economy strategy is to increase recycling and the use of industrial waste materials and side streams as secondary raw materials. In this study, a chemical precipitation method to simultaneously remove ammonium nitrogen and phosphate from the liquid phase of anaerobic digestate using calcined paper mill sludge was studied. Papermill sludge is a waste material that forms in the paper-making process. In addition, commercial calcium oxide (CaO) was used as a reference precipitant. The suitability of the formed precipitate’s composition f…

Kemimekaanisen puhdistamon toimintaan vaikuttavien tekijöiden hallinta

2007

The Design Towards a Collaborative Subscription Service : The Case of The Finnish Newspaper Industry

2020

The newspaper industry is challenged with its business models. To stabilize revenues, publishers opted for digital subscriptions for generating additional revenue streams. However, digital subscriptions showcase limited success. News aggregator platforms may promise publishers a pool of paying readers. But platform fees and the loss of customer relationships enact barriers among publishers to join. This study proposes a software prototype based on design science research to address the aforementioned shortcomings by deriving design principles for a collaborative subscription service. Building on the strategic alliance, digital platform and business model literature, this research aims to id…

The Design Towards a News Aggregator Subscription Service: Results from An Online Experiment

2021

The newspaper industry is on a quest to discover sustainable business models. Digital subscriptions are stable revenue generators for most publishers. That being said, smaller publishers face challenges. By not having the same resource base to offset costs compared to larger ones, small publishers are in a constant state of financial unpredictability. Another persistent problem among them is the high churn rate of subscriptions (i.e., the cancelation rate), which suggests a value misalignment between readers and service. New services like news aggregators may promise publishers a large pool of paying readers, as readers have access to diverse content by different publishers and are willing …

Apoptotic-like Leishmania exploit the host´s autophagy machinery to reduce T-cell-mediated parasite elimination

2015

Apoptosis is a well-defined cellular process in which a cell dies, characterized by cell shrinkage and DNA fragmentation. In parasites like Leishmania, the process of apoptosis-like cell death has been described. Moreover upon infection, the apoptotic-like population is essential for disease development, in part by silencing host phagocytes. Nevertheless, the exact mechanism of how apoptosis in unicellular organisms may support infectivity remains unclear. Therefore we investigated the fate of apoptotic-like Leishmania parasites in human host macrophages. Our data showed--in contrast to viable parasites--that apoptotic-like parasites enter an LC3(+), autophagy-like compartment. The compartm…