Search results for "Parietaria"

showing 6 items of 36 documents

Isolamento e Caratterizzazione Immunologica di un Allergene del Polline di Parietaria judaica

2011

Settore BIO/11 - Biologia MolecolareSettore MED/10 - Malattie Dell'Apparato Respiratorioallergie ige parietaria judaica
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DEVELOPMENT OF LIPID NANOSTRUCTURES CONTAINING A PARIETARIA POLLEN ALLERGEN.

2009

Settore CHIM/09 - Farmaceutico Tecnologico ApplicativoLIPID NANOSTRUCTURES PARIETARIA ALLERGEN.
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Detection and localisation of disulphide bonds in a synthetic peptide reproducing the sequence 1-30 of Par j 1.0101 by electrospray ionisation mass s…

2001

The structural characterisation of a synthetic peptide reproducing the sequence 1–30 of Par j 1.0101, a major allergenic protein present in the pollen of Parietaria judaica, by combined use of chemical and enzymatic cleavage, reversed-phase high-performance liquid chromatography (RP-HPLC) and electrospray ionisation mass spectrometry (ESI-MS), is described. Direct ESI-MS of the synthetic peptide after reaction with methyl iodide showed that the product is a mixture of two peptides: one form in which two out of the four cysteine residues present in the sequence are oxidised and a minor amount of another form in which all the cysteines are fully reduced. It was ascertained, using the combined…

Spectrometry Mass Electrospray IonizationElectrospray ionisation mass spectrometrySettore CHIM/10 - Chimica Degli AlimentiMolecular Sequence Data010401 analytical chemistryReproducibility of ResultsDisulphide bridgesGeneral Medicine010402 general chemistryPeptide Mapping01 natural sciencesAtomic and Molecular Physics and OpticsParietaria judaica0104 chemical sciencessynthetic peptide; Par j 1.0101; Parietaria judaica; disulphide bridges; structural characterisation; electrospray ionisation mass spectrometrySynthetic peptideTrypsinAmino Acid SequenceCyanogen BromideDisulfidesStructural characterisationPeptidesPar j 1.0101Chromatography High Pressure LiquidSpectroscopy
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Effects of Parietaria judaica on human lung microvascular endothelial cells

2008

cell adhesion moleculesendothelial cellParietaria judaica
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Anti-inflammatory effects of pre-seasonal Th1-adjuvant vaccine to Parietaria judaica in asthmatics

2011

Nicola Scichilone, Chiara Minaldi, Roberta Santagata, Salvatore Battaglia, Gaetana Camarda, Vincenzo Bellia Dipartimento Biomedico di Medicina Interna e Specialistica (Di.Bi.M.I.S.), Sezione di Pneumologia, University of Palermo, Palermo, ItalyBackground: The ultra-short course pre-seasonal allergy vaccine, containing appropriate allergoids with the adjuvant monophosphoryl lipid A (MPL), may be effective in treating allergic symptoms. Objective: To explore the timing of the immunological responses to the pre-seasonal allergy vaccine.Methods: Four subcutaneous injections of the active product (Pollinex Quattro) were administered to 20 Parietaria-sensitive intermittent asthmatics (M/F: 12/8; …

exhaled breath condensatelcsh:Immunologic diseases. AllergyPulmonary and Respiratory MedicinePediatricsmedicine.medical_specialtymedicine.drug_classmedicine.medical_treatmentSettore MED/10 - Malattie Dell'Apparato Respiratoriomedicine.disease_causeAnti-inflammatoryAllergenJournal of Asthma and AllergymedicineImmunology and AllergyExhaled breath condensateOriginal ResearchAsthmabiologybusiness.industryasthmabiology.organism_classificationmedicine.diseaseinflammationpollenallergen asthma immunotherapy inflammation pollen exhaled breath condensateImmunologyParietaria judaicaimmunotherapylcsh:RC581-607businessAdjuvantallergenJournal of Asthma and Allergy
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Production of an egg yolk antibody against Parietaria judaica 2 allergen

2009

Specific antibodies are essential tools for studying proteins as well as for diagnostic research in biomedicine. The egg yolk of immunized chicken is an inexpensive source of high-quality polyclonal antibodies. The 12-kDa Parietaria judaica 2 allergen was expressed as a fusion protein and was used to immunize Leghorn chickens. In this paper, we show, using 2-dimensional gel electrophoresis and immunoblotting, that chicken antibodies raised against a recombinant allergen can be used to recognize similar proteins from a pollen raw extract. Allergen identity was confirmed by nanoLC-nanospray-tandem mass spectrometry analysis. Our data demonstrate for the first time that a synergistic combinati…

food.ingredientImmunoglobulinsmedicine.disease_causeAntibodieslaw.inventionAllergenfoodlawYolkegg yolk antibodymedicineAnimalsGel electrophoresisDose-Response Relationship DrugbiologyGeneral MedicineAntigens Plantbiology.organism_classificationEgg YolkFusion proteinParietaria judaicaParietariaBiochemistryPolyclonal antibodiesImmunologyRecombinant DNAbiology.proteinParietaria judaicaPollenFemaleAnimal Science and ZoologyAntibodyChickensallergenPoultry Science
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