Search results for "Pathogens"
showing 10 items of 214 documents
One is not enough: On the effects of reference genome for the mapping and subsequent analyses of short-reads.
2020
Mapping of high-throughput sequencing (HTS) reads to a single arbitrary reference genome is a frequently used approach in microbial genomics. However, the choice of a reference may represent a source of errors that may affect subsequent analyses such as the detection of single nucleotide polymorphisms (SNPs) and phylogenetic inference. In this work, we evaluated the effect of reference choice on short-read sequence data from five clinically and epidemiologically relevant bacteria (Klebsiella pneumoniae, Legionella pneumophila, Neisseria gonorrhoeae, Pseudomonas aeruginosa and Serratia marcescens). Publicly available whole-genome assemblies encompassing the genomic diversity of these species…
Assessment of the antioxidant and antibacterial activities of different olive processing wastewaters.
2017
Olive processing wastewaters (OPW), namely olive mill wastewater (OMW) and table-olive wastewaters (TOW) were evaluated for their antibacterial activity against five Gram-positive and two Gram-negative bacteria using the standard disc diffusion and thin layer chromatography (TLC)-bioautography assays. Disc diffusion screening and bioautography of OMW were compared to the phenolic extracts of table-olive brines. Positive activity against S. aureus was demonstrated. The optimization of chromatographic separation revealed that hexane/acetone in the ratio of 4:6 was the most effective for phenolic compounds separation. A HPLC-MS analysis was performed showing that only two compounds, hydroxytyr…
From attachment to damage: defined genes of Candida albicans mediate adhesion, invasion and damage during interaction with oral epithelial cells.
2010
Candida albicans frequently causes superficial infections by invading and damaging epithelial cells, but may also cause systemic infections by penetrating through epithelial barriers. C. albicans is an unusual pathogen because it can invade epithelial cells via two distinct mechanisms: induced endocytosis, analogous to facultative intracellular enteropathogenic bacteria, and active penetration, similar to plant pathogenic fungi. Here we investigated the molecular basis of C. albicans epithelial interactions. By systematically assessing the contributions of defined fungal pathways and factors to different stages of epithelial interactions, we provide an expansive portrait of the processes an…
Paenibacillus larvae Chitin-Degrading Protein PlCBP49 Is a Key Virulence Factor in American Foulbrood of Honey Bees
2014
Paenibacillus larvae, the etiological agent of the globally occurring epizootic American Foulbrood (AFB) of honey bees, causes intestinal infections in honey bee larvae which develop into systemic infections inevitably leading to larval death. Massive brood mortality might eventually lead to collapse of the entire colony. Molecular mechanisms of host-microbe interactions in this system and of differences in virulence between P. larvae genotypes are poorly understood. Recently, it was demonstrated that the degradation of the peritrophic matrix lining the midgut epithelium is a key step in pathogenesis of P. larvae infections. Here, we present the isolation and identification of PlCBP49, a mo…
Survival of Bothryosphaeriaceae species after hot water treatment
2014
The use of Hot Water Treatment (HWT) in the grapevine propagation process has been shown to be A potenzially effective tool to control "Petri" and "Black foot" fungal pathogens. However, effects of HWT on Bothryosphaeriaceae after HWT in two different experiments. First, mycelial plugs contained in Eppendorf tubes with sterile distilled water were subjected to different combination of temperature (50-54°C) and exposure time (!5- 30- 45 minutes)in a hot water bath.In a second trial, the fungi were inoculated into 110 Richter rootstock canes, prviously subjected to HWT. Inoculated canes were incubated at 25°C for three weeks to allow for fungal colonization abd then were subjected at HWT in t…
Exploit biodiversity in viticultural systems to reduce pest damage and pesticide use, and increase ecosystem services provision: the Biovine project
2019
International audience; Organic vineyards still rely on large external inputs to control harmful organisms (i.e., pests). The BIOVINE project aims to develop natural solutions based on plant diversity to control pests and reduce pesticide dependence. The capability of plants of increasing the ecosystem resistance to pests and invasive species is a well-known ecosystem service. However, monocultures (including vineyards) do not exploit the potential of plant diversity. BIOVINE aims to develop new viticultural systems based on increased plant diversity within (e.g., cover crops) and/or around (e.g., hedges, vegetation spots) vineyards by planting selected plant species for the control of arth…
Concepts and methods to assess phytosanitary quality of soils
2005
Chapitre 9.4 : Plant-microbe interactions and soil quality; International audience
New perspectives and approaches in plant growth-promoting rhizobacteria research
2007
International audience; In the context of increasing international concern for food and environmental quality, use of Plant Growth-Promoting Rhizobacteria (PGPR) for reducing chemical inputs in agriculture is a potentially important issue. PGPR are root-colonizing bacteria that exert beneficial effects on plant growth and development, but they can be also employed in the control of plant pathogens, for enhancing the efficiency of fertilizers, and for degrading xenobiotic compounds. This book provides an update by renowned international experts on the most recent advances in the ecology of these important bacteria, the application of innovative methodologies for their study, their interactio…
Exposition des eaux souterraines peu profondes à Escherichia coli résistant aux antibiotiques : approche hydrochimique pour identifier les sources et…
2016
L'antibiorésistance est une préoccupation croissante en termes de santé humaine et animale au niveau international. Le but du travail est de suivre l'impact sur la qualité de l'eau dans le bassin versant de l’Ouche et évaluer la capacité des E. coli productrices de BLSE de type CTX-M à se propager et survivre dans les eaux. Une stratégie pour l’échantillonnage des eaux de surface et souterraines a été déployée selon les contextes hydrogéologiques, l'utilisation des terres et les STEP. L’approche hydrochimique s’appuie sur l’analyse des paramètres tels que les nitrates et le bore complétés par l’analyse de Terres rares dont le gadolinium et le suivi de produits pharmaceutiques (caféine, carb…
Influence de différentes pratiques agricoles sur la qualité et la santé des sols : étude de cas sur des vergers slovènes irrigués ou en agriculture b…
2011
Underestimation of soil properties and poor understanding of soil conditions can have many negative consequences, which results in quality or quantity of yield, soil degradation or even environmental pollution. According to importance of agricultural practices, our study focused on their impact on soil quality and health. The research took place from November 2003 to October 2007 in apple orchards in north-eastern Slovenia where two frequent agricultural practices were investigated: (i) drip irrigation on Calcaric Cambisol and its effects on structural stability and microbial biomass at Gačnik experimental station and (ii) combination of organic fertiliser (Campo guano) and liming in organi…