6533b862fe1ef96bd12c6de1
RESEARCH PRODUCT
Assessment of the antioxidant and antibacterial activities of different olive processing wastewaters.
Shiau Pin TanAbdellatif HafidiAbdelilah El-abbassiHajar KiaiOrla O'donovanPeter McloughlinMajdouline Belaqzizsubject
Thin-Layer Chromatography0301 basic medicineDPPHStaphylococcuslcsh:MedicineWastewaterPathology and Laboratory MedicineBiochemistryAntioxidantsMass Spectrometrychemistry.chemical_compoundPlant ProductsMedicine and Health SciencesStaphylococcus Aureuslcsh:ScienceChromatography High Pressure LiquidMultidisciplinaryBioautographyAntimicrobialsChromatographic TechniquesOlivesDrugsAgriculture04 agricultural and veterinary sciencesPlants040401 food scienceThin-layer chromatographyBacterial PathogensAnti-Bacterial AgentsHexaneChemistryBioassays and Physiological AnalysisMedical MicrobiologyPhysical SciencesPathogensAntibacterial activityResearch ArticleMicrobial Sensitivity TestsResearch and Analysis MethodsMicrobiologyVegetable OilsFruitsInhibitory Concentration 5003 medical and health sciences0404 agricultural biotechnologyPhenolsPicratesMicrobial ControlOleaAcetonePhenolsMicrobial PathogensPharmacologyChromatographyBacteriaBiphenyl Compoundslcsh:RChemical CompoundsOrganismsBiology and Life SciencesAgronomyTyrosolPlanar Chromatography030104 developmental biologychemistryAntibacterialsHydroxytyrosollcsh:QChromatography Thin LayerBiochemical AnalysisCrop Sciencedescription
Olive processing wastewaters (OPW), namely olive mill wastewater (OMW) and table-olive wastewaters (TOW) were evaluated for their antibacterial activity against five Gram-positive and two Gram-negative bacteria using the standard disc diffusion and thin layer chromatography (TLC)-bioautography assays. Disc diffusion screening and bioautography of OMW were compared to the phenolic extracts of table-olive brines. Positive activity against S. aureus was demonstrated. The optimization of chromatographic separation revealed that hexane/acetone in the ratio of 4:6 was the most effective for phenolic compounds separation. A HPLC-MS analysis was performed showing that only two compounds, hydroxytyrosol and tyrosol, were the predominant phenolic compounds in all OPW. The phenolic extract of OMW generated by a semi-modern process showed the highest free radical-scavenging activity (DPPH assay) compared to the other phenolic extracts. It is apparent from the present study that OPW are a rich source of antioxidants suitable for use in food, cosmetic or pharmaceutical applications.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-09-05 | PLoS ONE |