Search results for "Pathology"
showing 10 items of 8489 documents
Electron microscopic immunogold cytochemistry reveals chromogranin A confined to secretory granules of porcine Merkel cells
1990
By ultrastructural immunohistochemistry using the immunogold technique, immunoreactive (ir) Chromogranin A (CGA) was found to be confined to the secretory vesicles of porcine Merkel cells. CGA was present predominantly in the periphery of the electron-dense core and on the clear halo. These findings indicate that CGA is a regular constituent of Merkel cell secretory granules but probably not exclusively responsible for their electron opacity.
Chromogranin A in the Mammalian Merkel Cell: Cellular and Subcellular Distribution
1989
Chromogranin-A (CGA), which accounts for more than half the soluble matrix protein in secretory granules of various neuroendocrine cells, has a wide spectrum of potential biological roles and is considered an important marker of the diffuse neuroendocrine system (DNES). Light and electron microscopic immunohistochemistry of mammalian skin revealed that Merkel cells are exclusively CGA-immunoreactive (ir) and that the immunoreaction is localized in the secretory granules. This finding supports the classification of the Merkel cell as a member of the DNES. The CGA immunoreactivity was restricted to Merkel cells of pigs and humans. In human embryonic skin, CGA was expressed in Merkel cells as …
Tenascin gene expression in rat liver and in rat liver cells. In vivo and in vitro studies.
1991
Tenascin is a major glycoprotein constituent of the extracellular matrix with a strong affinity to fibronectin; its distribution is believed to be temporarily and spatially limited. Tenascin gene expression is increased during wound healing processes. As repair mechanisms in chronic liver diseases resemble wound healing we studied tenascin gene expression in rat liver and in isolated rat liver cells. In normal rat liver a tenascin specific antiserum stains sinusoidal cells with fiber-like prolongations, which at the same time are desmin-positive (ITO-cells). In the CCl4-acutely-damaged liver a strong tenascin staining is detected in cells located among the mononuclear cells of the inflammat…
Expression of tenascin in human cervical cancer--association of tenascin expression with clinicopathological parameters.
1999
Tenascin is an extracellular matrix glycoprotein, relevant for embryonal and fetal development, which is reexpressed in the stroma of benign and malignant tumors. Little is known about the molecular interaction of tenascin during neoplastic transformation and tumor progression in cervical cancer.We studied the expression of tenascin in normal tissue of the cervix uteri, cervical carcinoma in situ, and invasive cervical carcinoma in paraffin sections by immunohistochemistry using a monoclonal antibody. Tenascin immunoreactivity was compared with various prognostic parameters.In normal cervical tissue (n = 5) and in cervical carcinoma in situ (n = 10) only vessel walls showed a weak tenascin …
Primary Epithelial Ovarian Neoplasms: New Concepts Concerning Origin, Pathogenesis and Classification Based on Morphology, Immunomarkers, Molecular F…
2011
The World Health Organization (WHO) classification of ovarian tumors, which first appeared in 1983 and since then has undergone a number of revisions, is based on morphologic features as well as on the concept that each category of ovarian tumors develops from a specific ovarian cell. According to this histogenetic classification, all the epithelial ovarian neoplasms are derived from the ovarian surface epithelium and/or from ovarian inclusion cysts, which are lined by the above epithelial cells. In recent years, a new approach to morphologic data, increasing presumptive evidence that the cell of origin of most, if not all, ovarian epithelial tumors may be extraovarian, especially from fall…
Chapter 20 Multiple messenger candidates and marker substances in the mammalian Merkel cell – axon complex: a light and electron microscopic immunohi…
1988
Publisher Summary This chapter discusses multiple messenger candidates and marker substances in the mammalian merkel cell—axon complex. According to the APUD theory, cells of the diffuse neuroendocrine system (DNES) are essentially characterized by containing amines, peptides and marker substances like neuronspecific enolase (NSE) and chromogranin A (CGA). By the comparison of the histotopography of immunostained peptides, CGA and neuroendocrine markers, there was an expectation to find out the most appropriate immunohistochemical marker of the Merkel cell and to get further insight into the complex chemo-anatomy and possible functional spectrum of the Merkel cell -axon complex at various d…
Ontogeny of gonadotropin releasing hormone and gonadotropin immunoreactivity in brain and pituitary of normal and estrogen-treated guppies, Poecilia …
1987
Gonadotropin releasing hormone (GnRH) and gonadotropic hormone (GTH) were identified by immunohistochemistry in the brains and pituitaries of neonate, juvenile and adult guppies. GTH was present in some cells of the pars intermedia (pi) and proximal pars distalis (ppd) of all animals. GnRH was found in the perikarya of the nucleus olfactoretinalis. In the pituitaries of juvenile 30-day-old guppies, GnRH-immunoreactive cells existed in a "juvenile pattern", whereas in adult animals GnRH was recognized in only a few cells. GnRH-immunoreactive fibers were seen in the pituitaries of animals that were 30 days or older. In adult guppies, the ventral and lateral ppd (the gonadotropic region) conta…
Tubuloreticular structures (TRS) and cylindric confronting cisternae (CCC) in childhood dermatomyositis.
1989
Tubuloreticular structures (TRS) and cylindric confronting cisternae (CCC) have been observed in circulating lymphocytes and in the muscle of six children with dermatomyositis. The presence of TRS was seen in all cases investigated, the number of CCC increased in various cells with the severity of the disease. Extensive formation of TRS and CCC in childhood dermatomyositis probably reflects local or systemic alpha-interferon production and suggests that some viral factor is responsible for the disease.
Very highly prevalent Macracanthorhynchus hirudinaceus infection of wild boar Sus scrofa in Khuzestan province, south-western Iran
2006
Abstract An epidemiological and pathological study of Macracanthorhynchus hirudinaceus infection in a total of 50 wild boars Sus scrofa attila from cane sugar fields of Iranian Khuzestan was performed. The total prevalence of 64.0 % detected is the highest hitherto known by this acanthocephalan species in wild boars and may reflect a very high contamination of the farm lands studied as the consequence of the crowding of the wild boar population in cane sugar fields. Observations prove that this species is a wild boar gut wall perforating acanthocephalan. High burdens may become so pathogenic for the host individual as to be responsible for its elimination from the population. Thus, this aca…
Dental enamel: a new target of BPA
2012
Dental enamel: a new target of BPA. Journée des jeunes chercheurs