Search results for "Peroxidase"
showing 10 items of 435 documents
Seasonal changes in morpho-functional aspects of two Anemonia sulcata (Pennant, 1777) wild populations
2017
Marine benthic organisms can be used as indicators of the quality of environmental status and as monitoring tools to detect natural or anthropogenic perturbations. In temperate waters, metabolic and biochemical responses may be governed by physiological changes driven by seasonal factors. Gathering baseline information on the mechanisms underlying seasonal acclimation patterns is therefore a critical step towards the understanding of the physiological responses of biological indicators. In poikilothermic metazoans, the production of regulatory metabolic enzymes can be used as tools for deciphering the acclimation potential. The aim of this study was to characterize the natural seasonal vari…
The entangled multi-level responses of Mytilus galloprovincialis (Lamarck, 1819) to environmental stressors as detected by an integrated approach.
2021
Abstract Anthropogenic pressure adds up and interacts with the effects of climate change with a varying magnitude and potential changes depend on species’ Life History (LH) traits, local environmental conditions and co-occurrence of several stressors. Stressors exert negative effects on marine biota when acting as a single factor, but the effects may be amplified when more than one stressor work in combination, producing interacting effects on biodiversity and ecosystem functioning. The impairment of individual functional traits (FT) leads to strong rebounds on LH traits and this may have ecological consequences. No studies actually relate FT and antioxidant enzymes to multiple environmenta…
Can Indirect Herbicide Exposure Modify the Response of the Colorado Potato Beetle to an Organophosphate Insecticide?
2018
AbstractOrganisms live in complex multivariate environments. In agroecosystems, this complexity is often human-induced as pest individuals can be exposed to many xenobiotics simultaneously. Predicting the effects of multiple stressors can be problematic, as two or more stressors can have interactive effects. Our objective was to investigate whether indirect glyphosate-based herbicide (GBH) exposure of the host plant has interactive effects in combination with an insecticide (azinphos-methyl) on an invasive pest Colorado potato beetle (Leptinotarsa decemlineata Say). We tested the effects of GBH and insecticide on the survival, insecticide target genes expression (acetylcholinesterase genes)…
Light Conditions Affect NaCl-Induced Physiological Responses in a Clonal Plant Species Hydrocotyle vulgaris
2020
Abstract In order to understand if differences in light conditions can affect responses of a clonal plant species to increased soil salinity, the long-term effect of two substrate concentrations of NaCl on leaf growth, clonal plasticity and oxidative enzyme (peroxidase and polyphenol oxidase) activity in Hydrocotyle vulgaris L. plants grown at low, moderate and high light conditions was studied. H. vulgaris appeared to be a shade-tolerant species, since both leaf blade and petiole growth was inhibited at low light intensity. At low light intensity, H. vulgaris plants represented characteristics of halophytes with significant stimulation of leaf blade and petiole growth by NaCl, especially a…
Experimental climate warming alters the relationship between fungal root symbiosis and Sphagnum litter phenolics in two peatland microhabitats
2017
International audience; Belowground interactions between plants and microorganisms are involved in numerous ecosystems processes such as carbon and nutrient cycling. Understanding their responses to on-going climate warming is thus of paramount importance to better predict future ecosystem functioning. We hypothesized that climate warming alters the interactions between Sphagnum litter phenolics and the fungal root symbiosis of the Ericale plant Andromeda polifolia in a Jura mountain peatland (France). We initiate a climate warming treatment (+1°C) in April 2008 in two microhabitats (lawns and hummocks). We measured polyphenolic contents, mycorrhizal and dark septate endophyte (DSE) root co…
Underwater high frequency noise: Biological responses in sea urchin Arbacia lixula (Linnaeus, 1758)
2020
Marine life is extremely sensitive to the effects of environmental noise due to its reliance on underwater sounds for basic life functions, such as searching for food and mating. However, the effects on invertebrate species are not yet fully understood. The aim of this study was to determine the biochemical responses of Arbacia lixula exposed to high-frequency noise. Protein concentration, enzyme activity (esterase, phosphatase and peroxidase) and cytotoxicity in coelomic fluid were compared in individuals exposed for three hours to consecutive linear sweeps of 100 to 200 kHz lasting 1 s, and control specimens. Sound pressure levels ranged between 145 and 160 dB re 1μPa. Coelomic fluid was …
Nitric oxide production mediates oligogalacturonide-triggered immunity and resistance to Botrytis cinerea in Arabidopsis thaliana
2012
Nitric oxide (NO) regulates a wide range of plant processes from development to environmental adaptation. In this study, we investigated the production and/or function of NO in Arabidopsis thaliana leaf discs and plants elicited by oligogalacturonides (OGs) and challenged with Botrytis cinerea. We provided evidence that OGs triggered a fast and long lasting NO production which was Ca(2+) dependent and involved nitrate reductase (NR). Accordingly, OGs triggered an increase of both NR activity and transcript accumulation. NO production was also sensitive to the mammalian NO synthase inhibitor L-NAME. Intriguingly, we showed that L-NAME affected NO production by interfering with NR activity, t…
Colorimetic biosensing dispositive based on reagentless hybrid biocomposite: Application to hydrogen peroxide determination
2016
Abstract An efficient approach to enhance the performance of colorimetric biosensors has been developed. The biosensor is based on the co-immobilization of the reagent 3,3′,5,5′-teramethylbencidine (TMB) and the enzyme horseradish peroxidase (HRP) in a PDMS-TEOS-SiO2NPs support. The HRP, in presence of H2O2, catalyzes the oxidation of TMB, producing a blue color. The generated biosensor, doped with the substrate (TMB) and the enzyme (HRP) (entrapped or adsorbed), has been used to determine H2O2 in real samples. Firstly, the immobilization of TMB and HRP in the composite has been studied in order to find the best suitable configuration. The kinetic parameters Vmax (maximum reaction rate) and…
DHA protects PC12 cells against oxidative stress and apoptotic signals through the activation of the NFE2L2/HO-1 axis
2019
Docosahexaenoic acid (DHA) is an omega‑3 polyunsaturated fatty acid, derived mainly from fish oil. It is well known that DHA is present in high concentrations in nervous tissue and plays an important role in brain development and neuroprotection. However, the molecular mechanisms underlying its role remain to be fully elucidated. In this study, to enhance our understanding of the pathophysiological role of DHA, we investigated the possible neuroprotective mechanisms of action of DHA against hydrogen peroxide (H2O2)‑induced oxidative damage in a rat pheochromocytoma cell line (PC12). Specifically, we evaluated the viability, oxidation potential, and the expression and production of antioxida…
Cytoprotective and antioxidant properties of organic selenides for the myelin-forming cells, oligodendrocytes.
2018
Abstract Here a new series of twenty-one organoselenides, of potential protective activity, were synthesized and tested for their intrinsic cytotoxicity, anti-apoptotic and antioxidant capacities in oligodendrocytes. Most of the organoselenides were able to decrease the ROS levels, revealing antioxidant properties. Compounds 5b and 7b showed a high glutathione peroxidase (GPx)-like activities, which were 1.5 folds more active than ebselen. Remarkably, compound 5a diminished the formation of the oligodendrocytes SubG1 peak in a concentration-dependent manner, indicating its anti-apoptotic properties. Furthermore, based on the SwissADME web interface, we performed an in-silico structure-activ…