Search results for "Phagocyte"

showing 6 items of 66 documents

Apoptotic-like Leishmania exploit the host´s autophagy machinery to reduce T-cell-mediated parasite elimination

2015

Apoptosis is a well-defined cellular process in which a cell dies, characterized by cell shrinkage and DNA fragmentation. In parasites like Leishmania, the process of apoptosis-like cell death has been described. Moreover upon infection, the apoptotic-like population is essential for disease development, in part by silencing host phagocytes. Nevertheless, the exact mechanism of how apoptosis in unicellular organisms may support infectivity remains unclear. Therefore we investigated the fate of apoptotic-like Leishmania parasites in human host macrophages. Our data showed--in contrast to viable parasites--that apoptotic-like parasites enter an LC3(+), autophagy-like compartment. The compartm…

log.ph logarithmic phaseT-LymphocytesApoptosisMACS magnetic-associated cell sortingMacrophageMFI mean fluorescence intensityLeishmaniasisMOI multiplicity of infectionanti-inflammatoryLeishmaniaeducation.field_of_studyPhagocytesCFSE carboxyfluorescein succinimidyl esterTGFB transforming growth factorAcquired immune systemapoptotic-like LeishmaniaPS phosphatidylserinehuman primary macrophagesCell biologyβ; TT tetanus toxoidCorrigendumProgrammed cell deathautophagyPopulationAntigen presentationANXA5 annexin VBasic Science Research PapersBiologyPhagocytosisCM complete mediumMAP1LC3/LC3 microtubule-associated protein 1 light chain 3AnimalsHumansMHC major histocompatibility complexIF immunofluorescenceeducationMolecular Biologyimmune evasionPBMCs peripheral blood mononuclear cellsT-cell proliferationIntracellular parasiteMacrophagesstat.ph stationary phaseAutophagyLm LeishmaniaCell BiologyLeishmaniabiology.organism_classificationIL interleukinLAP LC3-associated phagocytosisLAPhMDM human monocyte derived macrophageAutophagy
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ACTH-Eosinopenie nach Splenektomie und Blockade des RES

1955

Es wird uber ACTH-Versuche an Meerschweinchen bei verschiedenem Funktionszustand des RES berichtet. Splenektomierte, splenektomiert und zusatzlich mit 6 cm3 sowie mit 18 cm3 Trypanblau blockierte Tiere zeigten jedesmal ein deutliches Absinken der Eosinophilen im peripheren Blut nach Injektion von 10 mg ACTH. Eine Aufhebung oder Abschwachung des eosinopenischen Effektes durch die Funktionseinschrankung des RES wurde nicht beobachtet.

medicine.anatomical_structurebusiness.industrymedicineSpleenGeneral MedicineMononuclear phagocyte systemAdrenocorticotropic hormonebusinessMolecular biologyZeitschrift für Die Gesamte Experimentelle Medizin
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Peritoneal cavity phagocytes from the teleost sea bass express a glucocorticoid receptor (cloned and sequenced) involved in genomic modulation of the…

2005

Abstract To gain further insight into the role of cortisol in Wsh innate immune responses, we cloned and sequenced a 2592 bp cDNA from sea bass (Dicentrarchus labrax) peritoneal leukocytes (PCLs) encoding a glucocorticoid receptor (DlGR1). The deduced aminoacid sequence displayed that DlGR1 belong to a multigenic family of steroid hormone receptors, and exhibited high homology (80%) to the Burton’s mouth breeder (Haplochromis burtoni) HbGR1. The DlGR1 functional domains presented homologies with those of several vertebrate species. In situ hybridization assay revealed that DlGR1 was expressed in macrophages and neutrophils from the peritoneal cavity. Since in a previous paper, sea bass PCL …

medicine.medical_specialtyDNA ComplementaryHydrocortisonemedicine.medical_treatmentMolecular Sequence DataDicentrarchus labrax; Peritoneal cavity leukocytes; Phagocytes; Hydrocortisone; RU486; Glucocorticoid receptor; DlGR1; GR cDNA sequence; GR mRNA expressionSequence HomologyGlucocorticoid receptorBiologyDlGR1Peritoneal cavitychemistry.chemical_compoundEndocrinologyGlucocorticoid receptorReceptors GlucocorticoidInternal medicinemedicineAnimalsDicentrarchus labraxPeritoneal cavity leukocyteAmino Acid SequenceSea bassReceptorPeritoneal CavityCells CulturedRespiratory BurstPhagocytesInnate immune systemDose-Response Relationship DrugZymosanZymosanMolecular biologyRespiratory burstSteroid hormoneGR mRNA expressionmedicine.anatomical_structureEndocrinologychemistryPhagocyteLuminescent MeasurementsGR cDNA sequenceAnimal Science and ZoologyBasshormones hormone substitutes and hormone antagonistsStress PsychologicalRU486
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The neuroimmune link in the bronchus-associated lymphoid tissue (BALT) of cat and rat: peptides and neural markers.

1991

This light microscopic immunohistochemical study investigates the distribution and target interrelations of nerve fibers in bronchus-associated lymphoid tissues (BALT) of rat and cat by using antisera against (1) the polyneuronal marker protein gene product 9.5 (PGP 9.5), (2) selected opioid and nonopioid peptides, and (3) the marker enzymes tyrosine hydroxylase (TH) and dopamine beta-hydroxylase (DBH). In both species, a similar distribution pattern of PGP, peptide, and catecholamine enzyme immunoreactive was observed. Anti-PGP 9.5 stained all nerve fibers (except some smaller, calcitonin gene-related peptide-immunoreactive (CGRP-ir) fibers presumably of the C-type) throughout the differen…

medicine.medical_specialtyTyrosine 3-MonooxygenaseLymphoid TissueNeuroimmunomodulationImmunologyImmunocytochemistryMolecular Sequence DataNeuropeptideNerve fiberSubstance PBronchiCell CommunicationDopamine beta-HydroxylaseBiologyCalcitonin gene-related peptideBehavioral Neurosciencechemistry.chemical_compoundNerve FibersInternal medicinemedicineAnimalsAmino Acid SequenceMast CellsPhagocytesTyrosine hydroxylaseEndocrine and Autonomic SystemsNeuropeptidesDendritic CellsMolecular biologyProenkephalinRatsmedicine.anatomical_structureEndocrinologychemistryCatsCholinergicUbiquitin ThiolesteraseBiomarkersBrain, behavior, and immunity
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Protein kinase C-inhibiting properties of the losartan metabolite EXP3179 make the difference.

2009

The inhibition of the renin-angiotensin axis with the angiotensin II (ATII) receptor blockers, such as losartan, candesartan, and valsartan, has been demonstrated, similar to angiotensin-converting enzyme inhibitors, to reduce mortality in patients with arterial hypertension, chronic congestive heart failure, and acute myocardial infarction.1 Initially, the ATII receptor antagonist losartan helped to demonstrate new classes of ATII receptors and substantially expanded our knowledge about the cardiovascular effects of the renin-angiotensin-aldosterone system and its effector peptide ATII. Researchers dealing with this compound soon revealed that, beyond its antihypertensive effects attribute…

medicine.medical_specialtymedicine.drug_classMetabolitePharmacologyLosartanchemistry.chemical_compoundInternal medicineInternal MedicinemedicineHumansReceptorProtein Kinase CPhagocytesNADPH oxidasebiologyNADPH OxidasesReceptor antagonistAngiotensin IICandesartanEndocrinologyLosartanchemistryValsartanMatrix Metalloproteinase 9Hypertensionbiology.proteinAngiotensin II Type 1 Receptor Blockersmedicine.drugHypertension (Dallas, Tex. : 1979)
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The use of an integrative approach to identify coelomocytes in three species of the genus Holothuria (Echinodermata)

2021

Coelomocytes in the Holothuroidea are traditionally identified according to their morphology through light, fluorescence, or electron microscopy. Former studies have typically used only one method, with few works combining two or more approaches. Studies using cytocentrifugation to study these cells are scarcer. Thus, for the first time, an integrative approach was used to compare coelomocytes in Holothuroidea. This approach consisted of living and stained cells, scanning electron microscopy (for spherule cells), and accurate morphometric analyses. Specifically, we used specimens of Holothuria grisea, Holothuria arenicola, and Holothuria tubulosa to test whether cytocentrifugation could be …

phagocytescytocentrifugationBIOLOGIA CELULARAnimal Science and Zoologyspherule cellsmaturation processsea cucumber
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