Search results for "Phosphor"
showing 10 items of 1952 documents
Tiam1 as a Signaling Mediator of Nerve Growth Factor-Dependent Neurite Outgrowth
2010
Nerve Growth Factor (NGF)-induced neuronal differentiation requires the activation of members of the Rho family of small GTPases. However, the molecular mechanisms through which NGF regulates cytoskeletal changes and neurite outgrowth are not totally understood. In this work, we identify the Rac1-specific guanine exchange factor (GEF) Tiam1 as a novel mediator of NGF/TrkA-dependent neurite elongation. In particular, we report that knockdown of Tiam1 causes a significant reduction in Rac1 activity and neurite outgrowth induced by NGF. Physical interaction between Tiam1 and active Ras (Ras- GTP), but not tyrosine phosphorylation of Tiam1, plays a central role in Rac1 activation by NGF. In add…
Rac1 and PAK1 are upstream of IKK-ε and TBK-1 in the viral activation of interferon regulatory factor-3
2004
The anti-viral type I interferon (IFN) response is initiated by the immediate induction of IFN beta, which is mainly controlled by the IFN-regulatory factor-3 (IRF-3). The signaling pathways mediating viral IRF-3 activation are only poorly defined. We show that the Rho GTPase Rac1 is activated upon virus infection and controls IRF-3 phosphorylation and activity. Inhibition of Rac1 leads to reduced IFN beta promoter activity and to enhanced virus production. As a downstream mediator of Rac signaling towards IRF-3, we have identified the kinase p21-activated kinase (PAK1). Furthermore, both Rac1 and PAK1 regulate the recently described IRF-3 activators, I kappa B kinase- and TANK-binding kina…
Rac1 protein signaling is required for DNA damage response stimulated by topoisomerase II poisons.
2012
To investigate the potency of the topoisomerase II (topo II) poisons doxorubicin and etoposide to stimulate the DNA damage response (DDR), S139 phosphorylation of histone H2AX (γH2AX) was analyzed using rat cardiomyoblast cells (H9c2). Etoposide caused a dose-dependent increase in the γH2AX level as shown by Western blotting. By contrast, the doxorubicin response was bell-shaped with high doses failing to increase H2AX phosphorylation. Identical results were obtained by immunohistochemical analysis of γH2AX focus formation, comet assay-based DNA strand break analysis, and measuring the formation of the topo II-DNA cleavable complex. At low dose, doxorubicin activated ataxia telangiectasia m…
KOSMOS 2017 Peru Side Experiment: nutrients, phytoplankton abundances, enzyme rates, photophysiology
2022
This data was collected during an short-term incubation experiment in March 2017 that investigated the response of a surface plankton community to upwelling. This experiment was carried in the framework of the SFB754-funded KOSMOS mesocosm study that took place in La Punta, Callao, Peru between February-April 2017. A total of six different treatments were used to disentangle chemical and biological characteristics of deep water that influence surface plankton blooms: 2 different deep water sources with different nutrient concentrations; 3 treatments to distinguish the effects of inorganic nutrients, organic nutrients and deep water microbial populations. Measured variables include inorganic…
Discharge management in fresh and brackish water RAS: Combined phosphorus removal by organic flocculants and nitrogen removal in woodchip reactors
2020
The current study combined P and N removal using organic flocculant chemicals and woodchip bioreactors in both freshwater and brackish water (7 ppm) recirculating aquaculture systems (RAS). The use of carbon (C) containing flocculant chemicals in the process was hypothesized to further stimulate C-demanding N removal (denitrification) in bioreactors. The trial of combined P and N removal consisted of four treatments: freshwater and brackish water RAS with and without the addition of supernatant from flocculation process to the woodchip reactor. Duplicate woodchip reactors were used per treatment and the trial was run for six weeks. 56 % and 49 % of P was removed from fresh and brackish slud…
Determination of Changes in the Quality of Surface Water in the River—Reservoir System
2021
Assessing the changing parameters of water quality at different points in the river–reservoir system can help prevent river pollution and implement remedial policies. It is also crucial in modeling water resources. Multivariate statistical analysis is useful for the analysis of changes in surface water quality. It helps to identify indicators that may be responsible for the eutrophication process of a reservoir. Additionally, the analysis of the water quality profile and the water quality index (WQI) is useful in assessing water pollution. These tools can support and verify the results of a multivariate statistical analysis. In this study, changes in water quality parameters of the Turawa r…
Biohiilen vaikutus torjunta-aineiden ja ravinteiden huuhtoutumiseen peltomaassa : esimerkkeinä glyfosaatti ja fosfori
2014
Tässä työssä tutkittiin kasvihuonekokein kolmessa eri lämpötilassa (300, 375 ja 475 ºC) tuotetun koivubiohiilen (80 tn/ha) vaikutusta glyfosaatin, aminometyylifosfonihapon (AMPA) ja fosforin (PO4-P) huuhtoutumiseen maaperästä. Lisäksi esiteltiin glyfosaatin ja fosforin keskinäistä vuorovaikutusta maaperässä sekä biohiilen tuotantoon liittyvien muuttujien vaikutusta torjunta-aineiden ja ravinteiden huuhtoutumiseen. Tutkimuksen mukaan maaperän biohiililisäykset vähentävät glyfosaatin huuhtoutumista keskimäärin 71 % ja AMPA:n huuhtoutumista keskimäärin 36 %. Biohiililisäykset eivät kuitenkaan vaikuttaneet tilastollisesti merkitsevästi fosforin huuhtoumaan. Biohiilen tuotantolämpötilalla ei oll…
Defying Stereotypes with Nanodiamonds: Stable Primary Diamondoid Phosphines
2016
International audience; Direct unequal C-H bond difunctionalization of phosphorylated diamantane was achieved in high yield from the corresponding phosphonates. Reduction of the functionalized phosphonates provides access to novel primary and secondary alkyl/aryl diamantane phosphines. The prepared primary diamantyl phosphines are quite air stable compared to their adamantyl and especially alkyl or aryl analogues. This finding is corroborated by comparing the singly occupied molecular orbital energy levels of the corresponding phosphine radical cations obtained by density functional theory computations.
Harnessing Molecular Fluorophores in the Carbon Dots Matrix: The Case of Safranin O
2022
The origin of fluorescence in carbon dots (C-dots) is still a puzzling phenomenon. The emission is, in most of the cases, due to molecular fluorophores formed in situ during the synthesis. The carbonization during C-dots processing does not allow, however, a fine control of the properties and makes finding the source of the fluorescence a challenging task. In this work, we present a strategy to embed a pre-formed fluorescent molecule, safranin O dye, into an amorphous carbonaceous dot obtained by citric acid carbonization. The dye is introduced in the melted solution of citric acid and after pyrolysis remains incorporated in a carbonaceous matrix to form red-emitting C-dots that are strongl…
Study of the role of phosphorylated pathway of serine biosynthesis in arabidopsis development and its connections with nitrogen and carbon metabolism
2016
En plantas se han descrito tres rutas diferentes de para la síntesis de serina: la ruta del glicolato, asociada a la fotorrespiración, y dos rutas independientes de ella, la ruta del glicerato y la ruta fosforilativa de biosíntesis de serina (PPSB). Como la ruta del glicolato es la más importante en términos cuantitativos, el papel de la PPSB ha sido ignorado hasta hace unos años. Esta tesis doctoral aporta nuevas perspectivas sobre la función de la PPSB tanto en el desarrollo como en el metabolismo de Arabidopsis. La PPSB se abastece de 3-fosfoglicerato (3-PGA) como sustrato, el cual puede ser suministrado por la glicolisis plastidial o citosólica. Se asume que la actividad de estas dos ru…