Search results for "Pill"

Functional feature of a novel model of blood brain barrier: Studies on permeation of test compounds

2001

Drug delivery to the central nervous system (CNS) is subject to the permeability limitations imposed by the blood-brain barrier (BBB). Several systems in vitro have been described to reproduce the physical and biochemical behavior of intact BBB, most of which lack the feature of the in vivo barrier. We developed a fully formed monolayer of RBE4.B immortalized rat brain microvessel endothelial cells (ECs), grown on top of polycarbonate filter inserts with cortical neuronal cells grown on the outside. Neurons induce ECs to synthesize and sort occludin to the cell periphery. Occludin localization is regulated by both compositions of the substratum and soluble signals released by cortical co-cu…

Micellar electrokinetic capillary chromatography for therapeutic drug monitoring of carbamazepine and its main metabolites.

1998

In carbamazepine (CBZ) therapy the concomitant monitoring of concentrations of CBZ and its metabolites is strictly recommended, primarily to avoid toxic side effects. Currently, clinical routine monitoring of CBZ is accomplished by high-performance liquid chromatography or immunological methods. In this study a micellar electrokinetic capillary chromatographic (MECC) method was developed for routine drug monitoring of CBZ and its main metabolites, carbamazepine 10,11-diol and carbamazepine 10,11-epoxide, in human serum or plasma samples. The MECC method enabled baseline separation of all analytes within 2.5 min. The assay revealed sufficient precision and sensitivity and the results of eith…

High-throughput capillary electrophoresis frontal analysis method for the study of drug interactions with human serum albumin at near-physiological c…

2004

The application of the short-end capillary injection to capillary electrophoresis frontal analysis (CE-FA) to study the interaction between basic, neutral and acid drugs towards human serum albumin (HSA) at near-physiological conditions is presented. The compounds selected display a wide range of binding affinities and the results obtained were in good agreement with those reported in the literature. An equation for the estimation of the number of primary binding sites and their corresponding affinity constants is developed isolating the experimentally measured variables in just one axis. The proposed CE-FA method to screen drug interactions with HSA under physiological conditions is simple…

Characterization of basic drug–human serum protein interactions by capillary electrophoresis

2006

Drug-protein interactions are determining factors in the therapeutic, pharmacodynamic and toxicological drug properties. The affinity of drugs towards plasmatic proteins is apparently well established in bibliography. Albumin (HSA) especially binds neutral and negatively charged compounds; alpha(1)-acid glycoprotein (AGP) binds many cationic drugs, lipoproteins bind to nonionic and lipophilic drugs and some anionic drugs while globulins interact inappreciably with the majority of drugs. In this paper, the characterization of the interaction between cationic drugs, beta-blockers and phenotiazines towards HSA, AGP, and both HSA + AGP mixtures of proteins under physiological conditions by CE-f…

Rapid in vitro test to predict ocular tissue permeability based on biopartitioning micellar chromatography.

2003

The drug permeability prediction across the ocular tissues is important in the development of new drugs and drug delivery strategies. Physicochemical characteristics of drugs, mainly acid-base character, hydrophobicity and the molecular size determine both their transport across the eye tissue barriers and their retention in biopartitioning micellar chromatography (BMC). An in vitro model able to describe and predict the whole cornea drug permeability is proposed. The model uses the retention of drugs in BMC and molecular weight (MW) as predictive variables. The relationships between drug retention data in BMC and their bibliographic permeability values in stroma, epithelium-plus-stroma and…

Microseparation techniques for the study of the enantioselectivity of drug-plasma protein binding.

2009

Stereoselectivity in protein binding can have a significant effect on the pharmacokinetic and pharmacodynamic properties of chiral drugs. The investigation of enantioselectivity of drugs in their binding with human plasma proteins and the identification of the molecular mechanisms involved in the stereodiscrimination by the proteins represent a great challenge for clinical pharmacology. In this review, the separation techniques used for enantioselective protein binding experiments are described and compared. An overview of studies on enantiomer–protein interactions, enantiomer–enantiomer interactions as well as chiral drug–drug interactions, including allosteric effects, is presented. The c…

Environmental Protection as a Trojan Horse of Criminal Law in the European First Pillar? A new Statement of the ECJ

2008

Dopo la storica sentenza del 13 settembre 2005, la Corte di Giustizia è intervenuta nuovamente a definire il riparto delle competenze tra Unione e Comunità in materia di diritto penale, annullando anche la decisione quadro sul rafforzamento della cornice penale per la repressione dell’inquinamento provocato da navi (caso C-440/05, Commissione c. Consiglio). Due erano le questioni fondamentali che attendevano una risposta da parte della Corte di Giustizia: 1) l’ammissibilità di direttive a contenuto penale per settori ulteriori rispetto a quello specifico della tutela dell’ambiente; 2) la definizione delle competenze della Comunità nella scelta del contenuto delle norme penali. Sul secondo p…

Studio videocapillaroscopico del microcircolo orale in pazienti ex-fumatori.

2011

Management in the 24/7-society raises concerns of fairness and social responsibility

2018

Purpose Despite the pressure on work-family polices arising from the increase in nonstandard working times in various sectors, only a few studies have addressed management practices in 24/7 workplaces. This paper aims to investigate the challenges Finnish managers face in meeting the various tensions stemming from nonstandard working hours and services operating 24/7. Two typical 24/7 work contexts are focused: the hospitality and retail industries and flexibly scheduled early childhood education and care (ECEC) services. The emphasis is on management practices relating to the planning of work shifts and children’s care schedules. Design/methodology/approach Study 1 comprises focus group i…

Flexibly scheduled early childhood education and care: experiences of Finnish parents and educators

2017

This study focuses on flexibly scheduled early childhood education and care (ECEC), an institutional childcare service for Finnish families where both parents, or a single parent, work non-standard hours. Although many countries nowadays offer extended hours day care, only Finland has a publicly provided, law-based system guaranteeing ECEC during non-standard as well as standard hours. We explore, drawing on parental survey data, what kinds of families use such services and when. Furthermore, we utilise web-survey data obtained from early educators to find out what they report as the main challenges involved in implementing flexibly scheduled ECEC. The results showed that single-parent fami…