Search results for "Pith"

showing 10 items of 1308 documents

Clinicopathological Significance of Syndecan-1 in Cholangiocarcinoma: A Study Based on Immunohistochemistry and Public Sequencing Data

2021

Background: Syndecan-1 (CD138

<i>SDC1</i>Pathologymedicine.medical_specialtyanimal structuresArticleSyndecan 1SDC103 medical and health sciences0302 clinical medicinemedicineLymph nodeIntrahepatic Cholangiocarcinoma030304 developmental biology0303 health sciencesbusiness.industryGallbladderRCancersyndecan-1General Medicinemedicine.diseasecarbohydrates (lipids)medicine.anatomical_structure030220 oncology & carcinogenesisBiliary Intraepithelial NeoplasiaMedicineImmunohistochemistrybiomarkerPancreasbusinesscholangiocarcinomaJournal of Clinical Medicine
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Polyoxypregnanes as safe, potent, and specific ABCB1-inhibitory pro-drugs to overcome multidrug resistance in cancer chemotherapy in vitro and in vivo

2021

Multidrug resistance (MDR) mediated by ATP binding cassette subfamily B member 1 (ABCB1) is significantly hindering effective cancer chemotherapy. However, currently, no ABCB1-inhibitory drugs have been approved to treat MDR cancer clinically, mainly due to the inhibitor specificity, toxicity, and drug interactions. Here, we reported that three polyoxypregnanes (POPs) as the most abundant constituents of Marsdenia tenacissima (M. tenacissima) were novel ABCB1-modulatory pro-drugs, which underwent intestinal microbiota-mediated biotransformation in vivo to generate active metabolites. The metabolites at non-toxic concentrations restored chemosensitivity in ABCB1-overexpressing cancer cells v…

ABCC1 ATP binding cassette subfamily C member 1IC50 half maximal inhibitory concentrationMultidrug resistancePharmacologyNADPH reduced nicotinamide adenine dinucleotide phosphateF bioavailabilitychemistry.chemical_compoundPCR polymerase chain reaction0302 clinical medicineMDR multidrug resistanceECL electrochemiluminescencet1/2 elimination half-lifeLC–MS liquid chromatography coupled with mass spectrometryN.D. not detectedGeneral Pharmacology Toxicology and PharmaceuticsBBB blood–brain barriermedia_commonATF3 activating transcription factor 30303 health sciencesChemistryABC ATP-binding cassetteNMPA National Medical Products AdministrationPXR pregnane X receptorSDS-PAGE sodium dodecyl sulfate-polyacrylamide gel electrophoresisHBSS Hankʹs balanced salt solutionABCB1Combination chemotherapyProdrugMarsdenia tenacissimaCmax peak concentrationPaclitaxelGAPDH glyceraldehyde-3-phosphate dehydrogenase030220 oncology & carcinogenesisBHI brain heart infusionOriginal ArticleAUC0–∞ area under plasma concentration vs. time curveMRT mean residence timeDrugmedia_common.quotation_subjectRM1-950Vd volume of distributionABCB1 ATP binding cassette subfamily B member 1UIC-2 mouse monoclonal ABCB1 antibodyABCG2 ATP binding cassette subfamily G member 2Combination chemotherapyCYP cytochrome P450 isozymePI propidium iodideTEER transepithelial electrical resistance03 medical and health sciencesPBS phosphate buffer salineFBS fetal bovine serumDox doxorubicinIn vivoPOP polyoxypregnanemedicine030304 developmental biologyEVOM epithelial tissue voltohmmeterTmax time for peak concentrationCancerLBE lowest binding energyPE phycoerythrinmedicine.diseaseMultiple drug resistancePolyoxypregnanePapp apparent permeabilityN.A. not applicableCancer cellH&E hematoxylin and eosinMDR1a multidrug resistance protein 1aTherapeutics. PharmacologyqPCR quantitative PCRM. tenacissima Marsdenia tenacissimaCL clearanceSD standard derivationActa Pharmaceutica Sinica B
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A new 3-d approach to determine functional morphology of cercopithecoid molars.

2005

Summary Functional relationships between diet and tooth morphology form an integral part of primatological and paleontological research. Previously, mostly two-dimensional parameters have been used to compare and interpret the complex crown morphology of cercopithecine and colobine molars. However, as teeth are three-dimensional objects, any dimensional reduction in describing their morphology must result in loss of information. In the current study we use a high resolution optical topometric system to record crown morphology in different wear stages in order to extract three-dimensional (3-d) parameters from virtual 3-d models. Structural parameters such as relief index, occlusal surface a…

Abrasion (dental)MolarModels Anatomicmedicine.medical_treatmentDentistryCrown (dentistry)Functional Lateralitystomatognathic systemFunctional morphologyChlorocebus aethiopsmedicineAnimalsAttritionOrthodonticsEnamel paintbusiness.industryCercopithecidaeGeneral Medicinemedicine.diseaseMolarTooth wearvisual_artvisual_art.visual_art_mediumCusp (anatomy)AnatomybusinessGeologyDevelopmental BiologyAnnals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft
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Myoepithelial cells are the main component in pleomorphic adenomas?

2007

Objective: The aim of this study was to quantify by immunohistochemistry the number of myoepithelial cells (MyECs) in pleomorphic adenomas (PAs). Material and methods: We retrieved the paraffin cubes of 27 PAs, new slides were done and they were stained with anti-S100 protein antibody. The amount of S-100 protein positive cells was quantified, their morphology was recorded and comparison among MyEC number with age, gender and involved gland were also done. Results: With S-100 protein, MyECs in normal salivary gland tissue were seen surrounding the ductual structures only. In the analysed PAs a mean of 27.4% of the neoplastic cells were positive to the antibody. With the exception of one PA,…

Adenoma pleomorfoUNESCO::CIENCIAS MÉDICASsalivary gland tumorscélulas mioepitelialestumores de glándulas salivales:CIENCIAS MÉDICAS [UNESCO]proteína S-100Pleomorphic adenomasmyoepithelial cellsS-100 protein
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WNT2 activation through proximal germline deletion predisposes to small intestinal neuroendocrine tumors and intestinal adenocarcinomas

2021

Abstract Many hereditary cancer syndromes are associated with an increased risk of small and large intestinal adenocarcinomas. However, conditions bearing a high risk to both adenocarcinomas and neuroendocrine tumors are yet to be described. We studied a family with 16 individuals in four generations affected by a wide spectrum of intestinal tumors, including hyperplastic polyps, adenomas, small intestinal neuroendocrine tumors, and colorectal and small intestinal adenocarcinomas. To assess the genetic susceptibility and understand the novel phenotype, we utilized multiple molecular methods, including whole genome sequencing, RNA sequencing, single cell sequencing, RNA in situ hybridization…

AdenomaAcademicSubjects/SCI01140DOMAINSadenokarsinoomaCANCER-RISKIn situ hybridizationsuolistosyövätAdenocarcinomaBiologyNeuroendocrine tumorsGermlineWnt2 Proteinperinnöllinen alttius03 medical and health sciences0302 clinical medicineWNT2GeneticsGenetic predispositionmedicineHumansIntestinal MucosaMUTATIONMolecular BiologyGenetics (clinical)030304 developmental biologypaksusuolisyöpäCARCINOID-TUMORS0303 health sciencesperinnölliset tauditCYSTIC-FIBROSISGeneral MedicineNATIONWIDEmedicine.diseaseIntestinal epithelium3. Good healthGENOMENeuroendocrine TumorsHyperplastic PolypSingle cell sequencing3121 General medicine internal medicine and other clinical medicine030220 oncology & carcinogenesisMAPCancer researchsyöpätaudit3111 BiomedicineGeneral Articlegeneettiset tekijätColorectal Neoplasms
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CT and MR Imaging Evaluation of Hepatic Adenoma

2006

Hepatic adenoma is a rare benign epithelial tumor that is usually encountered in young women who use oral contraceptives. It is clinically significant because of the risk of hemorrhage and of its low-grade malignancy potential. Adenomas usually are mildly hypervascular at contrast-enhanced CT and MR imaging, and heterogeneous due to the presence of hemorrhage, necrosis, calcifications and fat. The objective of our study was to illustrate the CT and MR imaging findings of hepatic adenoma.

AdenomaAdultmedicine.medical_specialtyNecrosisAdenomaComputed tomographyMalignancyDiagnosis DifferentialRare DiseasesmedicineHumansRadiology Nuclear Medicine and imagingBenign epithelial tumormedicine.diagnostic_testbusiness.industryLiver NeoplasmsMagnetic resonance imagingMiddle Agedmedicine.diseaseMagnetic Resonance ImagingMr imagingstomatognathic diseasesLiverFocal Nodular HyperplasiaFemaleRadiologymedicine.symptomTomography X-Ray ComputedbusinessLiver pathologyJournal of Computer Assisted Tomography
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Expression of glial filament protein (GFP) in nerve sheaths and non-neural cells re-examined using monoclonal antibodies, with special emphasis on th…

1986

We describe two novel monoclonal antibodies specific for glial filament protein (GFP), i.e., GF12.23 and GF12.24 (both IgG2a]. These cross-react over a broad range of species with epitopes located in the alpha-helical rod domain typical of all intermediate filament (IF) proteins. These monoclonal antibodies were used, in conjunction with other monoclonal GFP antibodies, rabbit antiserum to GFP, and various antibodies to other cytoskeletal proteins, to examine the occurrence of GFP in cells outside of the central nervous system of rodents, cows, and humans. We detected some scattered GFP-containing cells in the neural sheaths in some species but not in others, and we obtained different resul…

AdenomaCancer Researchmedicine.drug_classGuinea PigsAdenoma PleomorphicFluorescent Antibody TechniqueCross ReactionsMonoclonal antibodyEyeEpitopeEpitheliumSalivary GlandsGreen fluorescent proteinEpitopesSpecies SpecificityGlial Fibrillary Acidic ProteinmedicineAnimalsHumansVimentinIntermediate filamentMolecular BiologyMyelin SheathbiologyMyoepithelial cellAntibodies MonoclonalCell BiologyMolecular biologyRatsMicroscopy FluorescenceMonoclonalImmunologybiology.proteinHepatic stellate cellKeratinsCattleAntibodyDevelopmental BiologyDifferentiation; research in biological diversity
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Gastric adenomas: relationship between clinicopathological findings, Helicobacter pylori infection, APC mutations and COX-2 expression.

2006

Gastric adenomas are rare neoplastic growths characterized by localized polypoid proliferations of dysplastic epithelium that tend to progress to infiltrating adenocarcinoma. Therefore, the identification of molecular markers that could reliably recognize adenomas at risk of progression is advocated in the clinical management. In this study we investigated, in a series of gastric adenoma specimens from an area at high risk of gastric cancer, the relationship between clinicopathological characteristics of adenoma and Helicobacter pylori infection, APC mutational status, and COX-2 and the down-stream enzyme mPGES1 expression. Helicobacter pylori infection, detected in 24%, and 33% by histolog…

AdenomaMaleGenes APCAdenomaAdenocarcinomamedicine.disease_causegastric adenomaHelicobacter InfectionsHelicobacter pyloryStomach NeoplasmsmedicineHumansAPC mutationsAgedProstaglandin-E SynthasesAged 80 and overMutationbiologyHelicobacter pyloribusiness.industryCancerAPC mutations; COX-2; gastric adenoma; Helicobacter pyloriHistologyHematologyHelicobacter pyloriCOX-2Middle Agedmedicine.diseasebiology.organism_classificationdigestive system diseasesEpitheliumIntramolecular Oxidoreductasesmedicine.anatomical_structureOncologyDysplasiacyclooxygenase-2Cyclooxygenase 2Gastric MucosaMutationCancer researchAPC-mutations gastric adenoma Helycobacter pyloriAdenocarcinomaFemalebusiness
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Perspectives of Chromo and Magnifying Endoscopy

2003

The goal of every routine endoscopy in the gut is the early diagnosis of malignant and premalignant changes of the mucosa. Chromo- and magnifying endoscopes are exciting new tools and offer detailed analysis of the colonic mucosal surface and pit pattern architecture. This review summarizes recent advances in endoscopic characterization of colorectal lesions using magnification endoscopy and chromoendoscopy. Surface analysis of the colon using chromoendoscopy allows a prediction between non-neoplastic and neoplastic lesions with high specificity. The precise delineation of the borders and a more detailed macroscopic analysis of the lesions are further advantages. In particular, flat adenoma…

AdenomaPathologymedicine.medical_specialtyEndoscopeAdenomaRectumColonoscopySensitivity and SpecificityChromoendoscopymedicineHumansGastrointestinal NeoplasmsIntraepithelial neoplasiaStaining and Labelingmedicine.diagnostic_testbusiness.industryGastroenterologyColonoscopymedicine.diseaseUlcerative colitisEndoscopyMethylene Bluemedicine.anatomical_structureColitis UlcerativeGentian VioletbusinessForecastingJournal of Clinical Gastroenterology
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Immunolocalization of integrins in the normal and neoplastic colonic epithelium.

1993

Cryosections of normal colon (NC), tubular and villous adenomas (TA, VA), and variably differentiated colon adenocarcinomas (CA) were immunostained with monoclonal antibodies to alpha 1-6 and alpha v, and beta 1-4 integrin subunits; select samples were stained for cytokeratin (Ck) 20 and villin. In NC, alpha 2 staining was strongest in crypt cells; alpha 1,3 and alpha v, and beta 1,3 and beta 4, and Ck 20 and villin predominated in superficial enterocytes. In TA and VA, monolayered glands showed integrin, Ck 20 and villin patterns that differed slightly from both crypt and superficial enterocytes. Complex glands in VA showed decreased integrin staining and basal polarization; Ck 20 and vill…

AdenomaPathologymedicine.medical_specialtyIntegrinsColonCryptIntegrinFluorescent Antibody TechniqueAdenocarcinomadigestive systemEpitheliumExtracellular matrixImmunoenzyme Techniques03 medical and health sciencesCytokeratin0302 clinical medicineKeratinmedicineHumansTissue Distribution030304 developmental biologychemistry.chemical_classification0303 health sciencesbiologyCarcinomaMicrofilament ProteinsAntibodies MonoclonalCell DifferentiationGeneral MedicineEpitheliummedicine.anatomical_structurechemistry030220 oncology & carcinogenesisColonic Neoplasmsbiology.proteinImmunohistochemistryKeratinsVillinCarrier ProteinsVirchows Archiv. B, Cell pathology including molecular pathology
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