Search results for "Piwi"
showing 3 items of 23 documents
Isolation of Zebrafish Gonads for RNA Isolation
2013
Piwi proteins and piRNAs are abundant in the gonads of various animal species. Gonads from different developmental stages provide us information regarding the function of piRNAs and the PIWI pathway during germline development. Here we describe methods for gonad and germ cell preparation from different developmental stages of zebrafish. We also describe how to use these gonads to purify and characterize piRNAs.
Extensive nuclear gyration and pervasive non-genic transcription during primordial germ cell development in zebrafish.
2020
ABSTRACT Primordial germ cells (PGCs) are the precursors of germ cells, which migrate to the genital ridge during early development. Relatively little is known about PGCs after their migration. We studied this post-migratory stage using microscopy and sequencing techniques, and found that many PGC-specific genes, including genes known to induce PGC fate in the mouse, are only activated several days after migration. At this same time point, PGC nuclei become extremely gyrated, displaying general broad opening of chromatin and high levels of intergenic transcription. This is accompanied by changes in nuage morphology, expression of large loci (PGC-expressed non-coding RNA loci, PERLs) that ar…
Tīmekļa vietnes analīzes rīki
2015
Bakalaura darba mērķis ir iepazīties ar tīmekļa vietnes analīzes rīku piedāvātajām iespējām, noteikt rīku pielietojumu dažādos gadījumos un izpētīt to popularitāti Latvijā un ārpus Latvijas teritorijas. Daudzi informācijas tehnoloģijas speciālisti, kas strādā reālajos projektos, ieskaitot darba autoru, sastopas ar tīmekļa vietnes analīzes nepieciešamību. Parasti šim nolūkam izmanto vienu no pieejamiem rīkiem. Piemēram, darba autors savā ikdienas darbā izmanto Google Analytics rīku, lai analizētu tīmekļa vietni. Pamatoties uz personīgo pieredzi, darba autors veica rīku salīdzinājumu un to pielietošanas sfēras identificēšanu. Par galveno bakalaura darba rezultātu var uzskatīt to, ka darba nos…