Search results for "Placenta"

Metabolomics of Human Amniotic Fluid and Maternal Plasma during Normal Pregnancy

2016

Metabolic profiles of amniotic fluid and maternal blood are sources of valuable information about fetus development and can be potentially useful in diagnosis of pregnancy disorders. In this study, we applied 1H NMR-based metabolic profiling to track metabolic changes occurring in amniotic fluid (AF) and plasma (PL) of healthy mothers over the course of pregnancy. AF and PL samples were collected in the 2nd (T2) and 3rd (T3) trimester, prolonged pregnancy (PP) until time of delivery (TD). A multivariate data analysis of both biofluids reviled a metabolic switch-like transition between 2nd and 3rd trimester, which was followed by metabolic stabilization throughout the rest of pregnancy proba…

Deregulation of miR-324/KISS1/kisspeptin in early ectopic pregnancy: mechanistic findings with clinical and diagnostic implications

2019

[Abstract] BACKGROUND: Ectopic pregnancy is a life-threatening condition for which novel screening tools that would enable early accurate diagnosis would improve clinical outcomes. Kisspeptins, encoded by KISS1, play an essential role in human reproduction, at least partially by regulating placental function and possibly embryo implantation. Kisspeptin levels are elevated massively in normal pregnancy and reportedly altered in various gestational pathologic diseases. Yet, the pathophysiologic role of KISS1/kisspeptin in ectopic pregnancy has not been investigated previously. OBJECTIVE: The purpose of this study was to evaluate changes of KISS1/kisspeptin levels in ectopic pregnancy and thei…

CD133 expression in placenta chorioangioma presenting as a giant asymptomatic mass

2021

Background: Placental chorioangioma is the most common benign non-trophoblastic neoplasm of the placenta. Its clinical relevance lies in the size of the tumor since larger masses cause pregnancy complications, including an unfavorable neonatal outcome. Case presentation: We report the case of a 34-year-old second gravida and nullipara at the 35th week of gestation, admitted to the gynecological department for antibiotic-resistant fever. The cardiotocography performed during hospitalization showed an abnormal fetal pattern. A 2250 g newborn was delivered by cesarean section. No complications were observed during childbirth and postpartum was insignificant. On gross inspection a white fleshy …

HUMAN CHROMOSOME 4 SYNTENIC ASSOCIATIONS IN PLACENTAL MAMMALS

2009

Antitumor effect of B16 melanoma cells genetically modified with the angiogenesis inhibitor rnasin.

2001

The growth of new blood vessels is an essential condition for the development of tumors with a diameter greater than 1-2 mm and also for their metastatic dissemination. RNasin, the placental ribonuclease inhibitor, is known to have antiangiogenic activity through the inhibition of angiogenin and basic fibroblast growth factor. Nevertheless, the administration of the recombinant form of a protein poses several limitations; as a result, we have studied the antitumor effect of RNasin in a murine gene therapy model. RNasin cDNA was subcloned into the pcDNA3 expression vector, and the resulting recombinant plasmid was used to transfect the B16 murine melanoma cell line. An RNasin inverted constr…

Cardiac output following fetoscopic coagulation of major placental vessels in fetal sheep.

2008

Objectives To measure changes in cardiac output (CO) after partial cord occlusion in fetal sheep in order to investigate pathophysiological fetal adaptation mechanisms in a simulated acute placental insufficiency model under standardized conditions, with the aim of finding relevant methods for monitoring human fetuses during stress situations. Methods We used minimally invasive, percutaneous endoscopic techniques to close umbilical vessels in mid-gestational fetal sheep. Placental blood flow was reduced by preferentially closing first arterial and then the concomitant venous umbilical vessels within a short time interval. The investigations were carried out on 11 pregnant ewes at a median g…

Kathepsinaktivit�t in Placenten verschiedener Altersstufen

1954

In Placenten verschiedener Altersstufen wurde nach der Methode vonAnson die Kathepsinaktivitat bestimmt. Dabei zeigte sich, das gegenuber der Fruhgraviditat die Aktivitat am Ende der Schwangerschaft deutlich erniedrigt ist. Bemerkenswert ist die Gruppe der echten Schwangerschaftsubertragungen, die die niedrigsten Werte aufwiesen. Die Differenzen zwischen den einzelnen Gruppen sind statistisch signifikant. Die Bedeutung der Fermentaktivitat fur das Wachstum wird diskutiert.

The expression of CD68 in human umbilical cord mesenchymal stem cells: new evidences of presence in non-myeloid cell types.

2009

Since their first identification in bone marrow [2],MSC have attracted much attention for thei r potential todifferentiate towards several mature tissues. The efforts ofthe researchers aimed in finding new tissues, whichshould provide adequate cell numbers for regenerativemedicine applications (and between them, extraembryonicsources as umbilical cord and amniotic membrane, arebeing viewed with extreme interest).

Immunohistochemical location of HPL, SP1 and β-HCG in normal placentas of varying gestational age

1986

Sixty-four placentas at various gestational ages were examined by immunohistochemical stains for HPL, SP1 and beta-HCG according to a modified PAP method (Sternberger 1970). Syncytiotrophoblast cell layer was identified as the main site of synthesis. Extravillous immunohistochemical reactions for HPL and SP1 (but not for beta-HCG) were found in X-cells of the basal plate and in the intervillous trophoblast islands. These cell types would thus seem to be derived from trophoblast. Hofbauer-cells of villous connective tissue stained specifically for beta-HCG apparently because of HCG phagocytosis. The intensity of staining for HPL, SP1 and beta-HCG was evaluated semiquantitatively in the syncy…

Die proteingebundenen Kohlenhydrate in den einzelnen Zellfraktionen der menschlichen Placenta

1956

Zur Klarung der Frage, ob zwischen dem Gehalt an proteingebundenen Polysacchariden und dem Choriongonadotropin — ebenfalls einem Glykoproteid — in den einzelnen Zellfraktionen der Placenta eine Beziehung besteht, wurden 20 reife, frische Placenten und 5 Blasenmolen in ihre einzelnen Zellelemente (Zellkern, Mitochondrien, Cytoplasma+Mikrosomen) getrennt und chemisch auf ihren Gehalt an proteingebundenen Polysacchariden — Glucosamin, Mannose, Galaktose — untersucht. Wir fanden folgende Konzentrationen bei Placenten am Ende der Schwangerschaft (ausgedruckt in γ/mg N).Glucosamin: Gesamt=65, Zellkerne=36, Mitochondrien=47, Cytoplasma + Mikrosomen=99;Hexosen: Gesamt=319, Zellkerne=266, Mitochondr…