Search results for "Plant culture"
showing 10 items of 243 documents
Nuevos fármacos inspirados en Annonáceas
2014
Los metabolitos secundarios activos (MSA) juegan un papel importante en el descubrimiento de nuevos medicamentos. Moléculas naturales con esqueletos complejos, tales como las estatinas aisladas de Aspergillus terreus, o las acetogeninas específicas de la familia Annonaceae, no hubieran podido ser inventadas en ningún laboratorio. Los MSA aislados en Annonaceae, especialmente las acetogeninas y los alcaloides isoquinoleínicos, pueden ser considerados como fuente constante de inspiración para químicos, farmacólogos y para todos los investigadores interesados en el descubrimiento de una nueva familia de medicamentos. Active secondary metabolites (ASM) play a highly significant role in the drug…
Editorial: Highlights of ENPER 2019—European Network for Plant Endomembrane Research Meeting
2021
published
Morphogenic responsein vitroof epicotyl segments ofCitrus macrophylla
2008
Abstract The influence of explant orientation, contact of the cut surface with the medium, type of growth regulators and distance of epicotyl cuttings from the cotyledonary node (the part of the seedling where the cotyledons are attached) on in vitro morphogenesis of Citrus macrophylla (alemow) was evaluated. Maximum number of shoots was induced from epicotyl segments placed horizontally.
Micropropagation of Genista aetnensis [(Raf. ex Biv.)DC]
2015
Genista aetnensis [(Raf. ex Biv.)DC] is a large deciduous shrub or small tree native to the Italian islands of Sardinia and Sicily. Being winter hardy and characterized by high plasticity in altitude and ecology, the species is grown in gardens and landscaping, both for flower and for its attractive shape. Genista species are generally propagate by seed or semi hardwood cuttings. In this report an efficient in vitro technique for propagation of G. aetnensis was investigated. Multiple shoots were induced on nodal segments of a mature plant of Genista aetnensis . The Murashige and Skoog medium, augmented with different concentrations of benzyladenine either singly or in combination with indol…
Plant hormones and Agrobacterium tumefaciens strain 82.139 induce efficient plant regeneration in the cardenolide-producing plant Digitalis minor
2002
Summary Shoot formation in explants of Digitalis minor was achieved, through axillary bud proliferation and adventitious bud differentiation, by varying the amount and source of plant hormones. Shoot regeneration was also obtained after infection of D. minor with the wild-type Agrobacterium tumefaciens strain 82.139, which induced shooty tumours. These shoots were not transgenic, as revealed by nopaline assays and the use of a C58pMP90/T139GUS-INT strain harbouring the intron inactivated gusA gene. Plants were easily rooted and transplanted into the greenhouse. Shoot cultures of D. minor accumulated up to 226 μg cardenolides per g dry mass when cultured on agar-solidified medium. Cardenolid…
In vitro control of adventitious bud differentiation by inorganic media components in leaves of matureJuniperus oxycedrus
1995
The effects of changes in the concentration of macronutrients on BA-induced caulogenesis from leaves of matureJuniperus oxycedrus cultured on modified Murashige and Skoog or Schenk and Hildebrandt media are reported. The bud-forming capacity of the explants depended mainly on the ratios among the levels of ammonium, nitrate, and potassium. The most favorable media formulations for differentiation of adventitious buds were those with nitrate:potassium, ammonium:potassium, and nitrate:ammonium ratios near 1, around 0.1, and between 9 and 15, respectively. The total ionic strength of the media limited bud induction, but only when a disequilibrium of these ratios was produced.
Invasive Alien Plants in Sub-Saharan Africa: A Review and Synthesis of Their Insecticidal Activities
2021
Despite the cornucopia of agricultural, economic and ecological ramifications of invasive alien plant species (IAPs) in sub-Saharan Africa, studies on their potential use as bio-insecticides have not received adequate attention compared to the burgeoning plethora of literature on their use in ethnomedicine. In the current study, we review the existing, but scattered literature on the insecticidal activity of different parts of some IAPs; specifically those invasive in sub-Saharan Africa but with published literature from Africa and elsewhere. From our literature survey, we found that 69 studies from four continents (Africa, Asia, North America and South America) reported the insecticidal ac…
Changes of morphogenic competence in mature Pinus sylvestris L. buds in vitro.
2002
The effects of season and cold storage on morphogenic competence in mature Pinus sylvestris buds were investigated. Peroxidase and polyphenol oxidase activity were measured as markers of oxidative metabolism. No growth in vitro was observed on explants detached from the end of January until the beginning of March. Brachioblasts, each with a couple of needles, formed on 11 % of the buds without macrostrobili that were detached in early April and introduced immediately into culture. Of the explants detached in late July, 15 % formed shoots with brachioblasts and needles. The lowest activity of peroxidase and polyphenol oxidase in pine buds was observed from the end of April until the beginnin…
Micropropagation of Lavandula latifolia through nodal bud culture of mature plants
1996
Cultures of Lavandula latifolia Medicus were established from axillary buds of mature field-grown plants. Explants were initially cultured on media with two different macronutrient combinations and benzyladenine or kinetin added either individually or with naphthaleneacetic acid. Subsequently, explants were subcultured in Murashige and Skoog medium supplemented with 20% coconut milk, 0.57 μM indoleacetic acid and 8.88 μM benzyladenine. Shoot proliferation from axillary buds was not affected by seasonal fluctuations in the stock plants but depended on the macronutrient composition and on the type and concentration of cytokinin tested. Best results were obtained in explants initially cultured…
Micropropagation of adultLavandula dentataplants
1998
SummaryA protocol for in vitro propagation of adult Lavandula dentata plants has been achieved. Cultures were established by placing nodal segments on Murashige and Skoog medium containing BA, KIN, and NAA. Highest shoot multiplication rates were obtained when explants grown in the presence of 5.0 μM BA or 20 (JLM KIN were transferred to medium with 8.8 μM BA and 15% coconut milk. Multiplication efficiency through subcultures was significantly affected by the cytokinin concentration in the initial culture medium. Subculture reduced drastically the final number of shoots produced on nodal segments isolated from shoots grown in the presence of 2.0 μM BA or 40.0 μM KIN. Shoots were easily root…