Search results for "Preservation"

showing 10 items of 484 documents

Cryopreservation of Digitalis obscura selected genotypes by encapsulation-dehydration

2001

Shoot-tips from several genotypes of the cardenolide-producing perennial shrub Digitalis obscura L. were successfully cryopreserved using the encapsulation-dehydration technique. Precultivation on MS medium containing 0.5 M sucrose, followed by 2.5 h dehydration (final weight 30 %) induced shoot regrowth in 42 % of cryopreserved shoot-tips. Cold-hardening of the in vitro cultures before sucrose treatment dramatically increased shoot recovery up to 86 %. The optimized cryopreservation protocol was then employed using different shoot cultures from five D. obscura genotypes. Responses to cryopreservation depended mainly on the genotype, best results being obtained when shoot tips from HU3 and …

SucroseDNA PlantGenotypeScrophulariaceaeAcclimatizationDigitalis obscuraPharmaceutical ScienceCapsulesCryopreservationAnalytical ChemistrySomaclonal variationchemistry.chemical_compoundMurashige and Skoog mediumDrug DiscoveryBotanyDesiccationCryopreservationPharmacologyDigitalisbiologyOrganic Chemistryfungifood and beveragesbiology.organism_classificationRandom Amplified Polymorphic DNA TechniqueRAPDCold TemperatureHorticultureComplementary and alternative medicinechemistryShootMolecular MedicinePlant Shoots
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Freezing without surrounding cryomedium preserves the endothelium and its function in human internal mammary arteries

2005

Abstract Purpose Cryopreserved human blood vessels may become important tools in bypass surgery. Optimal cryopreservation of an arterial graft should, therefore, preserve both histological and physiological characteristics of smooth muscle and endothelium comparable to the unfrozen artery. Methods Rings from human internal mammary arteries (IMA) were investigated in vitro either unfrozen or after immersion into a cryomedium (RPMI 1640 containing 1.8 M Me2SO and 0.1 M sucrose) and cryostorage with and without surrounding medium. Results In unfrozen IMA, neither contractile responses to noradrenaline (NA) nor endothelium-dependent relaxant responses to acetylcholine (ACH) was modified after e…

SucrosePathologymedicine.medical_specialtyEndotheliumBiologyGeneral Biochemistry Genetics and Molecular BiologyCryopreservationAndrologyNorepinephrinechemistry.chemical_compoundCryoprotective AgentsFreezingmedicineHumansDimethyl SulfoxideEndotheliumMammary ArteriesPhorbol 1213-DibutyrateProtein Kinase CProtein kinase CCryopreservationDose-Response Relationship DrugDimethyl sulfoxideTemperatureMuscle SmoothGeneral MedicineAcetylcholineCulture MediaCold TemperatureEnzyme ActivationMicroscopy ElectronDose–response relationshipmedicine.anatomical_structurechemistryCarcinogensMicroscopy Electron ScanningMammary arteryEndothelium VascularTissue PreservationGeneral Agricultural and Biological SciencesAcetylcholinemedicine.drugArteryCryobiology
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Effects of an innovative dipping treatment on the cold storage of minimally processed Annurca apples

2007

The effect of trehalose as an edible coating on minimally processed Annurca apple slices was studied during cold storage. The edible coating was prepared by dipping the fruit in a solution containing trehalose at 0.8%, sucrose at 1.0% and sodium chloride at 0.1%. During storage at 6 degrees C the following parameters were monitored: weight loss, colour (hue angle (h degrees) and whitening index (WI)), firmness, malic and ascorbic acids, polyphenol content.. microstructure by scanning electron microscopy (SEM) and microbial count. The results showed that such a coating reduced the browning phenomena; in fact the WI and h degrees values were significantly lower in coated samples than untreate…

SucroseScanning electron microscopeSodiumFood preservationCold storagechemistry.chemical_elementGeneral Medicineengineering.materialTrehaloseAnalytical Chemistrychemistry.chemical_compoundchemistryBiochemistryCoatingBrowningengineeringFood scienceFood Science
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Cryopreservation of white mulberry (Morus alba L.) by encapsulation-dehydration and vitrification

2011

Shoot apices of in vitro-grown plantlets of white mulberry, Morus alba L. cv Florio, were cryopreserved using either encapsulation-dehydration or vitrification. For encapsulation-dehydration, alginate beads containing apices were dehydrated for 1, 3, 5 or 7 days in a liquid medium containing various sucrose concentrations (0.5, 0.75, 1.0 or 1.25 M). Bead desiccation was performed using silica gel for either 0, 4, 6, 8, 9 or 14 h. For vitrification, apices were directly immersed for either 5, 15, 30 or 60 min in a vitrification solution (PVS2). Following encapsulation-dehydration, treatment of alginate beads with 0.75 M sucrose was more effective in promoting re-growth of explants after imme…

SucroseSilica gelHorticultureBiologymedicine.diseaseCryopreservationAlginate beads PVS2 Re-growth Silica gel Sucrosechemistry.chemical_compoundHorticulturechemistryShootBotanymedicineVitrificationDehydrationDesiccationExplant culture
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“Water Association” Band in Saccharide Amorphous Matrices: Role of Residual Water on Bioprotection

2021

Saccharides protect biostructures against adverse environmental conditions mainly by preventing large scale motions leading to unfolding. The efficiency of this molecular mechanism, which is higher in trehalose with respect to other sugars, strongly depends on hydration and sugar/protein ratio. Here we report an Infrared Spectroscopy study on dry amorphous matrices of the disaccharides trehalose, maltose, sucrose and lactose, and the trisaccharide raffinose. Samples with and without embedded protein (Myoglobin) are investigated at different sugar/protein ratios, and compared. To inspect matrix properties we analyse the Water Association Band (WAB), and carefully decompose it into sub-bands,…

SucroseSucrosePopulationwaterLactose010402 general chemistry01 natural sciencesCatalysisArticleInorganic Chemistrylcsh:Chemistrychemistry.chemical_compoundRaffinose0103 physical sciencesAnimalsTrisaccharideHorsesPhysical and Theoretical ChemistryRaffinoseLactoseeducationSugarinfrared spectroscopyMolecular Biologylcsh:QH301-705.5Spectroscopytrehalosechemistry.chemical_classificationeducation.field_of_study010304 chemical physicsOrganic ChemistryGeneral MedicineMaltoseTrehalose0104 chemical sciencesComputer Science ApplicationschemistryChemical engineeringlcsh:Biology (General)lcsh:QD1-999myoglobinbiopreservation
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Digital preservation in institutional repositories: a systematic literature review

2021

Purpose This paper aims to carry out a literature review on the implementation of digital preservation policies, strategies and actions by institutional repositories. The objective is to identify, out of the published experiences, at which level they are fulfilling the function of ensuring the long-term availability of the deposited materials. Design/methodology/approach Using a systematic literature review methodology, a total of 21 articles from international refereed journals published between 2009 and 2020 are reviewed. Findings The research production on this subject is very limited. The scarce number of published articles proves that the interest of repository managers has been focus…

Systematic reviewDigital preservationPolitical science05 social sciences0509 other social sciencesLibrary and Information Sciences050905 science studies050904 information & library sciencesData scienceEducationInformation Systems
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La scoperta, sistemazione e conservazione della grande Iscrizione di Gortina, nell'isola di Creta (1884-1921): la protezione delle testimonianze e le…

2013

La scoperta e la conservazione della Grande Iscrizione di Gortina a Creta, da parte degli studiosi italiani, fra la fine del XIX sec. e i primi decenni del XX sec., rappresentò un grande riconoscimento scientifico-culturale per la giovane nazione italiana. Nel passato, l’isola di Creta era stata legata alla Repubblica di Venezia e anche questo rapporto favorì l’invio a Creta del giovane epigrafista F. Halbherr, il quale fra notevoli peripezie rinvenne, assieme all’epigrafista tedesco E. Fabricius, la famosa l’iscrizione nell’antica città di Gortina. L’iscrizione, fra le più antiche e complete finora conosciute in tutta Europa, conteneva le norme sulla famiglia, l’eredità e in generale i dir…

The discovery and preservation of the Great Inscription of Gortyna in Crete by Italian researchers in the late nineteenth century. and the first decades of the twentieth century. was an important cultural and scientific recognition for the young Italian nation. In the past the island of Crete had been tied to the Republic of Venice and this report also favored sending in Crete the young epigraphist F. Halbherr who among considerable vicissitudes came to together to the epigraphist german E. Fabricius the inclusion of the famous ancient city of Gortyna. The inscription among the oldest and most comprehensive so far known throughout Europe the rules contained on the family's legacy in general and the rights and duties of some of the people of the Minoan civilization (480-450 BC). The inscription was carved on blocks organic limestone a restored ancient building which later in Roman times was turned into odeon. The Laws of Gortyn were engraved on the remains of the wall forming the passageway in front of the steps of the odeon. The essay traces the events that led to the discovery and subsequent storage made by Halbherr especially with the help of archaeologist L. Pernier and finally with the help of architect E. Stefani who designed a modern cover on the top of the ambulatory of the segments containing the inscription. This coverage was made to perform during the First World War on the basis of the Italian project from the Greek Government in order to protect the membership from possible war damage but especially from vandalism. The roof brick clay of the Laws of Gortyna still serves its function and ensures the use of the monument bearing witness to the preservation of Italian culture in Greece and more generally in the world. But in Sicily in the remains of the Villa del Casale in Piazza Armerina (Enna) its elegant protection (built in 1957) is representative of a whole period of culture of the Italian restoration is removed and replaced because it has not been able to make a ongoing maintenance is due. A bad example of conservation and management of a UNESCO monument from which arise after the work for the construction of new roofs an equally insulting to the archaeological heritage and scenic Italian and world.Settore ICAR/19 - Restauro
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Tommaso Maria Napoli Utriusque Architecturae Compendium Roma 1688 Breve trattato sulle fabbriche civili e militari e la conservazione delle architett…

2013

Il volume Tommaso Maria Napoli Utriusque Architecturae Compendium Roma 1688 Breve trattato sulle fabbriche civili e militari e la conservazione delle architetture del frate domenicano Tommaso Maria Napoli, vuole contribuire ad accrescere le nostre conoscenze sui principi che dovevano sottendere alla realizzazione dell’architettura civile e militare del periodo barocco e tardo barocco, così come espressi nel breve trattato scritto dal frate architetto, nato a Palermo, Tommaso Maria Napoli (1659-1725), e pubblicato a Roma alla fine del Seicento. Alla traduzione dal latino del trattato, redatta da esperti (Fabio Zarbo e Pietro Zarbo), è inclusa un’interessante ricerca, redatta congiuntamente c…

The volume Tommaso Maria Napoli Utriusque Architecturae Brief Compendium 1688 Rome treaty on civil and military factories and preservation of the architecture of the Dominican friar Tommaso Maria Napoli wants to help increase our understanding of the principles that should underpin the realization of civil and military baroque and late baroque period as expressed in the short treatise written by the monk architect born in Palermo Tommaso Maria Napoli (1659-1725) and published in Rome at the end of the seventeenth century. This translation from the Latin of the treaty drawn up by experts (Fabio Zarbo e Pietro Zarbo) included is an interesting research drafted jointly with Giuseppe Tantillo status of some of the architecture without realizing or attributed to the architect Tommaso Maria Napoli. In fact some cards are attached to the volume on the substance and state of preservation of the cathedral of the Assumption of Mary in Dubrovnik (Croatia) of the villas Valguarnera and Palagonia in Bagheria (near Palermo Italy) of the monument to the Immaculate and one of the towers of the church of San Domenico in Palermo (Italy). The volume is enriched by the presentation and afterword by Francesco Tomaselli that among other things starting from the definition of Restoration expressed by Tommaso Maria Napoli in his book presents a stimulating reflection on the meaning of Restoration. The essay Tommaso Maria Napoli Utriusque Architecturae Compendium 1688 ... is the first of the series Monument Document for the conservation of the architectural heritage and environment: Theory knowledge interventions publisher Aracne in Rome with director Francesco Tomaselli and with Comittee Scientific and editorial international.Settore ICAR/19 - Restauro
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Cytokine- and Interferon-Modulating Properties of Echinacea spp. Root Tinctures Stored at −20°C for 2 Years

2007

Echinacea spp. phytomedicines are popular for treating upper respiratory infections. The purpose of this investigation was to examine the immunomodulatory properties of Echinacea tinctures from seven species after being stored at -20 degrees C for 2 years. Two experimental techniques were employed using human peripheral blood mononuclear cells (PBMC). In the first set of experiments, PBMCs were stimulated in vitro with tinctures alone and assayed for proliferation and production of interleukin-10 (IL-10), IL-12, and tumor necrosis factor-alpha (TNF-alpha). In the second set of experiments, subjects were immunized with influenza vaccine. PBMCs from vaccinated individuals were stimulated in v…

Time FactorsDrug StorageImmunologyPeripheral blood mononuclear cellPlant RootsVirusArticleEchinaceaCytokine interferon Echinacea immunologyEchinacea (animal)Interferon-gammaImmune systemSpecies SpecificitySettore BIO/13 - Biologia ApplicataInterferonVirologymedicineHumansInterferon gammaCells CulturedCell ProliferationCryopreservationTraditional medicinebiologyPlant ExtractsTumor Necrosis Factor-alphaCell Biologybiology.organism_classificationInterleukin-12Interleukin-10Interleukin 10AlcoholsImmunologyInterleukin 12Leukocytes MononuclearCytokinesmedicine.drug
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Metabolism of propafenone and verapamil by cryopreserved human, rat, mouse and dog hepatocytes: comparison with metabolism in vivo

2003

In the present study we examined the metabolism of [(14)C]propafenone (P) and [(14)C]verapamil (V) using cryopreserved human, dog (Beagle), rat (Sprague-Dawley) and mouse (NMRI) hepatocytes. The percentage ratios of the metabolites were identified after extraction by HPLC with UV and radioactivity detection. Phase-II metabolites were cleaved using beta-glucuronidase. Metabolism of the drugs by cryopreserved hepatocytes was compared with that in the respective species in vivo. All phase-I and -II metabolites known from in vivo experiments: 5-hydroxy-P (5-OH-P); 4'-hydroxy-P (4'-OH-P); N-despropyl-P (NdesP) and the respective glucuronides, were identified after incubation with cryopreserved h…

Time FactorsPropafenoneIn Vitro TechniquesPharmacologyCryopreservationRats Sprague-DawleyHydroxylationMicechemistry.chemical_compoundDogsGlucuronidesPropafenoneSpecies SpecificityIn vivomedicineAnimalsHumansIncubationAgedCryopreservationPharmacologyChemistryGeneral MedicineMetabolismMiddle AgedIn vitroRatsVerapamilBiochemistryHepatocytesVerapamilAnti-Arrhythmia Agentsmedicine.drugNaunyn-Schmiedeberg's Archives of Pharmacology
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