Search results for "Pressure"
showing 10 items of 4493 documents
Synthesis of recombinant atrial natriuretic peptide (rANP) using hybrid fusion protein-phage fr coat/ANP (CP/ANP).
1997
Abstract Baumanis, V., I. Jansone, A. Skangals, I. Mandrika and V. Berzins. Synthesis of recombinant atrial natriuretic peptide (rANP) using hybrid fusion protein-phage fr coat/ANP (CP/ANP). Peptides 18(8) 1229–1235, 1997.—Recombinant atrial natriuretic peptide (rANP) was expressed in and isolated from E. coli. rANP was purified using HPLC. Amino acid analysis, partial sequencing, and molecular mass were determined. Fused protein was used to rise polyclonal antibodies and to develop of immunoenzymatic assays of rANP and CP/ANP. Experiments were designed to study rANP effects on isolated rabbit aortic strips and to examine hypotensive, diuretic, and natriuretic activity, as well as renal cre…
ATP distribution and localization of mitochondria in Suberites domuncula (Olivi 1792) tissue
2011
SUMMARY The metabolic energy state of sponge tissue in vivo is largely unknown. Quantitative bioluminescence-based imaging was used to analyze the ATP distribution of Suberites domuncula (Olivi 1792) tissue, in relation to differences between the cortex and the medulla. This method provides a quantitative picture of the ATP distribution closely reflecting the in vivo situation. The obtained data suggest that the highest ATP content occurs around channels in the sponge medulla. HPLC reverse-phase C-18, used for measurement of ATP content, established a value of 1.62 μmol ATP g–1 dry mass in sponge medulla, as opposed to 0.04 μmol ATP g–1 dry mass in the cortex, thus indicating a specific and…
Detection of a plant enzyme exhibiting chlorogenate-dependant caffeoyltransferase activity in methanolic extracts of arbuscular mycorrhizal tomato ro…
2012
When Glomus intraradices-colonised tomato roots were extracted in methanol at 6 degrees C, chlorogenic acid (5-caffeoylquinic acid), naturally present in the extract, was slowly converted by transesterification into methyl caffeate. The progress of the reaction could be monitored by HPLC. The reaction only occurred when the ground roots were left in contact with the hydro-alcoholic extract and required the presence of 15-35% water in the mixture. When the roots were extracted in ethanol, chlorogenic acid was transformed to ethyl caffeate in the same conditions. The reaction was also detected in Glomus mosseae-colonised tomato root extracts. It was also detectable in non-mycorrhizal root ext…
Development of a low-cost piezo film-based knock sensor
2003
It is well known that spark advance is a key parameter in spark ignition engine management. Increasing fuel cost and emission regulation strictness require a higher engine efficiency, which can be improved by an accurate regulation of the spark advance. Under high load conditions, an optimal spark advance choice leads the engine to run next to the knock limit, so the management and control system needs to be equipped with a knock sensor in order to preserve the engine from damage. The authors developed a low-cost knock sensor whose sensing element is a thin washer of polyvinylidine fluoride (PVDF), a fluoropolymer characterized by a great piezoelectric e ect if polarized. The sensor has be…
Byssochlamys nivea inactivation in pineapple juice and nectar using high pressure cycles
2009
The aim of this work was to assess the inactivation of Byssochlamys nivea ascospores in pineapple juice and nectar by combining pressure sequences involving high pressure cycles with relatively mild thermal processing. The effect of 550 and 600 MPa sustained pressures (holding time of 15 min), combinations of sustained pressures and pressure pulses (holding time of 10 s), and pressure cycles (two, three and five cycles of 550 and 600 MPa for 7.5, 5 and 3 min, respectively), at 20, 40, 60, 70, 80 and 90 °C were compared. B. nivea ascospores were inactivated by applying sustained a pressure of 600 MPa at 90 °C for 5 min (juice) and 15 min (nectar), and three and five cycles of pressure at 600…
Research on the internal pressure behavior of metal gas distribution pipelines with different types of tubing defects
2017
The paper aims to approach an important subject related to natural gas distribution networks which, depending on the expansion of the localities, are composed of intercommunicating pipes, pressure reducing stations and branch connections fittings. The urban networks are the most complex ones and the rural areas networks are the simplest. However, irrespective of their installation, they must meet the safety operating requirements as much as possible. According to standards, all these components must be tight and pressure resistant. In this regard, we intend to approach a very important issue related to the behavior of the tubular steel material showing corrosion and/or material defects, and…
Prenatal Clinical Assessment of sFlt-1 (Soluble fms-like Tyrosine Kinase-1)/PlGF (Placental Growth Factor) Ratio as a Diagnostic Tool for Preeclampsi…
2013
Background: Aim of the study was a critical assessment of the clinical validity of the prenatal determination of sFlt-1/PlGF for preeclampsia (PE), pregnancy-induced hypertension (PIH), and proteinuria. Our analysis was based on a specificity of 95 % and a sensitivity of 82 % for the prediction of preeclampsia, as described by Elecsys (Roche). Methods: In this retrospective study the ratio of the prenatal antiangiogenic factor sFlt-1 (soluble fms-like tyrosine kinase-1) to the proangiogenic factor PIGF (placental growth factor) was analyzed using the electrochemiluminescence immunoassay of Elecsys (Roche Diagnostics, Mannheim, Germany) in 173 pregnant women. Sixty-three women with PE, 34 wo…
Analysis of Phenolic Compounds in Commercial
2019
Industrial hemp (Cannabis sativa L. Family Cannabaceae) contains a vast number of bioactive relevant compounds, namely polyphenols including flavonoids, phenolic acids, phenol amides, and lignanamides, well known for their therapeutic properties. Nowadays, many polyphenols-containing products made of herbal extracts are marketed, claiming to exert health-promoting effects. In this context, industrial hemp inflorescence may represent an innovative source of bioactive compounds to be used in nutraceutical formulations. The aim of this work was to provide a comprehensive analysis of the polyphenolic fraction contained in polar extracts of four different commercial cultivars (Kompoti, Tiborszal…
Heart rate and blood pressure responses to a competitive role-playing game
2001
The effects of the outcome of competitive encounters on physiological parameters have been studied, especially testosterone levels, but hardly on other systems that, however, present a high sensitivity to stress. This study assessed the effect of a competitive game on heart rate (HR) and blood pressure (BP) in a sample of university students. In addition, the influence of anxiety and attributions of the outcome was also explored. Only winners significantly showed a rise in HR during the competition followed by a decrease along the posttask phase in addition to more internal attributions. On the contrary, the average HR for losers during the competition was lower compared with their baseline…
Tridiagonal preconditioning for Poisson-like difference equations with flat grids: Application to incompressible atmospheric flow
2011
AbstractThe convergence of many iterative procedures, in particular that of the conjugate gradient method, strongly depends on the condition number of the linear system to be solved. In cases with a large condition number, therefore, preconditioning is often used to transform the system into an equivalent one, with a smaller condition number and therefore faster convergence. For Poisson-like difference equations with flat grids, the vertical part of the difference operator is dominant and tridiagonal and can be used for preconditioning. Such a procedure has been applied to incompressible atmospheric flows to preserve incompressibility, where a system of Poisson-like difference equations is …